Results 101 to 110 of about 540,123 (302)

The Renaturation of Denatured DNA

Biophysical Journal, 1962
The kinetics of renaturation of heat-denatured DNA from E. coli and pneumococcus have been examined by ultraviolet absorption measurements. The molecularity of the reaction was assessed by three independent treatments of the data, and all lead to the conclusion that renaturation is essentially first order at 60 degrees ; at 70 degrees and 80 degrees ...
Cavalieri, Liebe F., Small, Theodore, Sarkar, N.   +2 more
openaire   +2 more sources

Nanotherapies for Atherosclerosis: Targeting, Catalysis, and Energy Transduction

Advanced Healthcare Materials, EarlyView.
Atherosclerosis management is hindered by poor drug targeting and plaque heterogeneity. Nanotechnology overcomes these barriers via three core strategies: (1) target‐engineered nanocarriers that achieve lesion‐specific precision via ligand modification, biomimetic camouflage, stimuli‐responsive release, and self‐propelling nanomotors; (2) catalytic ...
Yuqi Yang, Jingguo Qu, Tonghuan Xiao, Xinyang Han, Michael Tin‐Cheung Ying   +4 more
wiley   +1 more source

Pulses protein quality control at different storage conditions for further protein extraction – a review

Julius-Kühn-Archiv, 2018
The storage conditions are of extreme importance with regards to grains (cereal & pulses) components (carbohydrates, lipids, proteins) preservation and quality for industry (that may interfere to whole process and quality of the final product).
Dutra, Milena O., Soares, Carlos E. S., Ferreira, Bárbara C. F., Ribeiro, Christiano W. R., Scussel, Vildes M.   +4 more
doaj   +1 more source

Chemical shifts in denatured proteins: Resonance assignments for denatured ubiquitin and comparisons with other denatured proteins

Journal of Biomolecular NMR, 2001
Chemical shift assignment is reported for the protein ubiquitin denatured in 8M urea at pH 2. The variations in 15N chemical shifts of three different proteins (ubiquitin, disulfide reduced, carboxymethylated lysozyme, all-Ala-alpha-lactalbumin), all without disulfides and denatured in 8M urea at pH 2 are compared to 'random coil shifts' of small model
Peti, W, Smith, L, Redfield, C, Schwalbe, H   +3 more
openaire   +3 more sources

Tuning the Testicular Microenvironment for Enhancing Human Sertoli Cells Maturation and Functionality In Vitro

Advanced Healthcare Materials, EarlyView.
An in vitro testicular model is developed by generating connective tissue equivalents from human dermal fibroblast‐derived microtissues and coupling them with human Sertoli cells or human Sertoli cell spheroids. This engineered microenvironment supports Sertoli cell maturation and functionality, providing a promising platform for studying human ...
Annachiara Scalzone, Giorgia Imparato, Chiara Ausilio, Valentina Mollo, Paolo Antonio Netti   +4 more
wiley   +1 more source

Simultaneous Electrochemical Detection of NGF and proNGF Under Native Conditions Using Molecularly Imprinted Polymers: Toward Point‐of‐Care Diagnosis of Alzheimer's Disease

Advanced Healthcare Materials, EarlyView.
This study relies on the development of a new sensor that can detect two closely related proteins involved in Alzheimer's disease at the same time. Unlike traditional methods, it works in natural conditions and does not require sample pretreatment. The device is highly sensitive and was successfully tested on patient samples, offering promises for ...
Giulia Siciliano, Alfredo De Cillis, Elena Clabassi, Francesco Ferrara, Maria Serena Chiriacò, Antonio Turco, Clarissa Loiola, Chiara Zecca, Maria Teresa Dell'Abate, Giancarlo Logroscino, Giuseppe Gigli, Francesca Malerba, Elisabetta Primiceri   +12 more
wiley   +1 more source

Protein Denaturation in Foam

Journal of Colloid and Interface Science, 1999
The aim of this study was to elucidate the mechanism by which protein molecules become denatured in foam. It was found that damage to the protein is mainly due to surface denaturation at the gas-liquid interface. A fraction of the molecules adsorbed do not refold to their native state when they desorb.
Clarkson, JR, Cui, Z, Darton, R
openaire   +4 more sources

A Cryoprotectant‐Compatible Nanoporous Platform for Stable and Scalable Delivery of Biopharmaceuticals

Advanced Materials, EarlyView.
A cryoprotectant‐compatible nanoporous platform enables ambient‐stable and scalable delivery of gene editing therapeutics. By combining hierarchical pore architecture with optimized lyophilization chemistry, the system preserves Cas9‐RNP activity post‐freeze‐drying.
Sian Lee, Seongchan Kim, Jooyeun Chong, Min‐gyeong Kim, Kayeong Lim, Nan‐Ee Lee, Jahyun Koo, Jiheong Kang, Hyojin Lee   +8 more
wiley   +1 more source

Synchronizing the Osteochondral Regeneration Process through Spatial Patterning of Stable and Hypertrophic Cartilage Organoids

Advanced Materials, EarlyView.
Zonated osteochondral assembloid implants are engineered through scaffold‐free spatial assembly of induced pluripotent stem cell (iPSC)‐derived chondrocyte organoids and periosteum‐derived osteogenic organoids. Implanted into full‐thickness rat osteochondral defects, they promote hyaline‐like cartilage repair, support subchondral bone formation with ...
Liuqi Peng, Isaak Decoene, Hanna Svitina, Ioannis Papantoniou   +3 more
wiley   +1 more source

DNA denaturation and wetting in the presence of disorder

, 2012
We present a precise equivalence of the Lifson-Poland-Scheraga model with wetting models. Making use of a representation of the former model in terms of random matrices, we obtain, in the limit of weak disorder, a mean--field approximation, that shows a ...
Kunz, Hervé, Livi, Roberto
core   +1 more source