Results 111 to 120 of about 3,437,879 (280)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

High gas-pressure apparatus for nonlinear X-ray propagation and reshaping via stimulated X-ray Raman scattering

Scientific Reports
We describe an experimental setup for non-linear interaction and propagation of ultrashort and intense x-ray free-electron laser (XFEL) pulses in a dense gas.
Alexander Magunia, Marc Rebholz, Tommaso Mazza, Alberto De Fanis, Thomas M. Baumann, Sergey Usenko, Nils Rennhack, Kai Li, Marc Simon, Marcus Agåker, Jan-Erik Rubensson, Michael Meyer, Linda Young, Christian Ott, Thomas Pfeifer   +14 more
doaj   +1 more source

Particle-Scale Local Flow Disturbances Induced by Feed-Particle Size and Shape in a Gas–Solid Fluidized Bed

Separations
During coal beneficiation in a gas–solid fluidized bed, coarse feed particles act as moving intruders whose size and shape can reorganize the local gas–solid flow field.
Zengqiang Chen, Shuai Zhou, Yadong Zhang, Guangqing Zhu, Bin Li, Dingjie Zhang, Enhui Zhou   +6 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Digging into the Interior of Hot Cores with ALMA (DIHCA). VI. The Formation of Low-mass Multiple Systems in High-mass Cluster-forming Regions

The Astrophysical Journal
Most stars form in multiple systems, with profound implications in numerous astronomical phenomena intrinsically linked to multiplicity. However, our knowledge about the process of how multiple stellar systems form is incomplete and biased toward nearby ...
Qiu-yi Luo, Patricio Sanhueza, Stella S. R. Offner, Fernando Olguin, Adam Ginsburg, Fumitaka Nakamura, Kaho Morii, Yu Cheng, Kei E. I. Tanaka, Junhao Liu, Tie Liu, Xing Lu, Qizhou Zhang, Kotomi Taniguchi, Piyali Saha, Shanghuo Li, Xiaofeng Mai   +16 more
doaj   +1 more source

Method and apparatus for evanescent filed measuring of particle-solid separation

, 2004
Evanescent wave scattering by a scanning probe in a scanning probe microscope is utilized to determine and monitor separation between a scanning probe and a sample.

core  

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Observing Colocated Neutral and Ionized Gas-phase Iron Depletion in the Magellanic Clouds

The Astrophysical Journal
Depletion is the observed phenomenon where gas-phase elemental abundances are reduced through accretion onto dust grains. We measure neutral gas-phase elemental abundances (S, Fe) in the Magellanic Clouds along 33 sightlines using high-resolution UV ...
Yun Qi Li, Jessica K. Werk, Caleb R. Choban, Julia Roman-Duval, Kirill Tchernyshyov, J. Xavier Prochaska, Doyeon A. Kim, Arianna S. Long   +7 more
doaj   +1 more source