Results 91 to 100 of about 1,098,050 (261)
LIBERACIÓN DE LOTES PARA PRODUCTOS EN DESARROLLO/ BATCH RELEASE FOR INVESTIGATIONAL PRODUCTS
Revista Científica de Ingeniería Industrial, 2012 Este artículo describe un procedimiento de liberación de productos en desarrollo del Centro de Inmunología Molecular. El Procedimiento incorpora el conocimiento y la complejidad de la investigación y desarrollo (I+D), análisis de riesgo, correlaciones y ...
Daymara González-Fuentes +3 more
doaj
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry
FEBS Letters, EarlyView.EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri +5 more
wiley +1 more source
IEEE AccessComprehensive data on machines is essential for digitization in the industry. Since standardized machine elements are used in most machines, integrating them with sensors provides the opportunity to acquire data comprehensively from in-situ.
Julian Peters +6 more
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Effect of γ-radiation on current transport in a gallium arsenide field-effect transistor
Технологія та конструювання в електронній апаратурі, 2002 Through modeling and experimental studies, it is shown that a multi-order increase in the channel current of a gallium arsenide field-effect transistor under the influence of γ-radiation is caused by the appearance of secondary currents in the substrate ...
F. D. Kasimov, A. E. Liutfalibekova
doaj
A Design Space of Control Coordinate Systems in Telemanipulation
IEEE AccessTeleoperation systems map operator commands from an input device into some coordinate frame in the remote environment. This frame, which we call a control coordinate system, should be carefully chosen as it determines how operators should move to get ...
Yeping Wang +2 more
doaj +1 more source