Results 231 to 240 of about 93,770 (286)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Quantitative method comparison in non-targeted analysis using cascade classification. [PDF]

Anal Bioanal Chem
Ranasinghe Arachchige NPR, Sapkota K, Rivera O, Patel K, Kim HJ, Patabandige M, Perera T, Young JA.   +7 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Single-photon detection in few-layer NbSe₂ superconducting nanowires. [PDF]

Nat Commun
Zugliani L, Palermo A, Scaparra B, Patra A, Wietschorke F, Metuh P, Paralikis A, De Fazio D, Kastl C, Flaschmann R, Munkhbat B, Müller K, Finley JJ, Barbone M.   +13 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Toward XRF-like chemical sensitivity in the laboratory: first hyperspectral x-ray imaging demonstration using the novel CITIUS detector. [PDF]

Sci Rep
Trapani VD, Thibault P, Arfelli F, Honjo Y, Ozaki K, Nishino H, Joti Y, Hatsui T, Orsini F, Menk RH.   +9 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Computer Vision-Based Information Extraction from Electrical Assets: A Systematic Literature Review. [PDF]

Sensors (Basel)
Alvarez-Murillo C, Ospina-Bedoya A, Branch-Bedoya JW, Zapata Madrigal GD, García Sierra R.   +4 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Depth-Sensitive Optical Sensing for Non-Invasive Measurement of Human Muscle Activity. [PDF]

Sensors (Basel)
Matsuo K, Bandara DSV, Nogami H, Arata J.   +3 more
europepmc   +1 more source