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Quantification of Detergents Complexed with Membrane Proteins
Most membrane proteins studies require the use of detergents, but because of the lack of a general, accurate and rapid method to quantify them, many uncertainties remain that hamper proper functional and structural data analyses.
V. Chaptal+19 more
semanticscholar +1 more source
Comparison of the α and β isomeric forms of the detergent n-dodecyl-D-maltoside for solubilizing photosynthetic complexes from pea thylakoid membranes [PDF]
Mild non-ionic detergents are indispensable in the isolation of intact integral membrane proteins and protein-complexes from biological membranes. Dodecylmaltoside (DM) belongs to this class of detergents being a glucoside-based surfactant with a bulky ...
Amunts+63 more
core +1 more source
This study aimed to investigate the toxicological effects of the LAS-C12 on Mugil platanus (mullet). Fishes exposed to 1.0 mg.L-1 for 24, 48, 72, 96 and 120 hours presented significant increase in specific routine metabolism.
Edison Barbieri
doaj +1 more source
The decomposition of detergent powder samples in a microwave oven and autoclave was evaluated. To establish the best experimental conditions a 2(5) factorial design was performed, varying the conditions in autoclave and microwave digestion and flow ...
Fabíola Soraia Vital Campos Barbosa da Silva+3 more
doaj +1 more source
Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi+103 more
core +4 more sources
Guided Tissue Regeneration in Heart Valve Replacement: From Preclinical Research to First-in-Human Trials [PDF]
Heart valve tissue-guided regeneration aims to offer a functional and viable alternative to current prosthetic replacements. Not requiring previous cell seeding and conditioning in bioreactors, such exceptional tissue engineering approach is a very ...
Gerosa, Gino, Iop, Laura
core +4 more sources
Fatty acids: a versatile and sustainable source of raw materials for the surfactants industry
Fatty acids have been used as a major source for the hydrophobic part of surfactants since the discovery of soap, more than 4,500 years ago. Despite the wide variety of different hydrophobes that have become available during recent decades, fatty acids ...
Pel Axel
doaj +1 more source
Structure formation in binary mixtures of lipids and detergents: Self-assembly and vesicle division
Self-assembly dynamics in binary surfactant mixtures and structure changes of lipid vesicles induced by detergent solution are studied using coarse-grained molecular simulations.
Noguchi, Hiroshi
core +1 more source
Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update [PDF]
The quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolute complex protein samples.
Adessi+95 more
core +4 more sources
Most MmpLs transport lipids and glycolipids of the complex mycomembrane of mycobacteria. This study shows that MmpL10 from Mycobacterium smegmatis, the transporter of the trehalose polyphosphate precursor, harbors a coiled‐coil‐like extension. Biochemical and electron microscopy studies demonstrate that the coiled‐coil enables MmpL10 to trimerize.
Julie Couston+7 more
wiley +1 more source