Results 171 to 180 of about 915 (260)

Design and Manufacture of Multifunctional 3-D Smart Skins with Embedded Sensor Networks for Robotic Applications. [PDF]

Sensors (Basel)
Ransom E, Chen X, Mangram W, Nasrollahi A, Topac T, Chang FK.   +5 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Entrance-channel plugging by natural sulfonamide antibiotics yields isoform-selective carbonic anhydrase IX inhibitors: an integrated in silico/ in vitro discovery of the lead SB-203207. [PDF]

BMC Chem
Kamel EM, Ahmed NA, Maodaa S, Abuamarah BA, Othman SI, Abalkhail A, Alkhayl FFA, Lamsabhi AM.   +7 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Surface to Surface Development between 3D Sculptured Surfaces by Using Planar Development

Korean Journal of Computational Design and Engineering, 2017
openaire   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A droplet microfluidics-based platform for generating target-specific, natively-paired immune libraries and identifying potent and developable antibodies. [PDF]

Sci Rep
Ramasubramanian A, Deveney BT, Clark SA, Boutin P, Keepseagle K, Riahi S, Lao T, Salemi M, Kathuria S, Amengual-Rigo P, Dimova D, Marcovici L, Hopke J, Newton J, Mix K, Kramer K, Erasimus H, Furtmann N, Mottet G, Suslov N, Mahendra A, Pemmaraju SR, Chowdhury PS, Weitz DA, Heyman JA.   +24 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Expanding the reaction toolbox for nanoscale direct-to-biology PROTAC synthesis and biological evaluation. [PDF]

RSC Med Chem
Stevens R, Shrives HJ, Cryan J, Klimaszewska D, Stacey P, Burley GA, Harling JD, Battersby DJ, Miah AH.   +8 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source