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Visualizing the translational activation of a particular mRNA in zebrafish embryos using in situ hybridization and proximity ligation assay

open access: yesSTAR Protocols
Summary: Fertilized eggs initiate translation of stored mRNAs in spatially and temporally controlled manners. Here, we present a protocol for visualizing spatial and temporal translation in zebrafish embryos by fluorescence in situ hybridization and ...
Keisuke Sato, Tomoya Kotani
doaj  

Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen

open access: yesSTAR Protocols
Summary: Protein synthesis could control spermatogenic cell fate transitions. Here, we present a protocol for visualization and quantification of newly synthesized proteins by click-chemistry-based immunofluorescence within specific spermatogenic cell ...
Dingfeng Zou   +4 more
doaj  

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