EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation
Advanced Science, Volume 12, Issue 25, July 3, 2025.Following ionizing radiation, eccDNA‐mediated VPS41 amplification slightly increases its expression but fails to prevent apoptosis. Introducing exogenous eccDNA or VPS41 enhances VPS41‐KAI1 interaction, promoting lysosomal degradation of KAI1. This process inhibits apoptotic signaling, enhancing cell survival and resistance to radiation‐induced damage.
Bin Shi +12 more
wiley +1 more source
Computer-Assisted Modeling and Examination of Protein-Molecule Complexes
CHIMIA, 1984 Three applications of the new Roche Interactive Molecular Graphics system are outlined. They include (i) the computer-assisted fitting of various brodimoprim derivatives into the active sites of L.casei and E.coli dihydrofolate reductases (DHFR), (ii ...
Klaus Müller
doaj +1 more source
BioFactors, Volume 51, Issue 4, July/August 2025.Mycophenolate mofetil, alanosine, trimetrexate, and sulfamethoxazole mimic methotrexate's effects by enhancing AICAR‐induced AMPK activation and glucose uptake in L6 myotubes. Collectively, our results suggest that IMP metabolism may serve as a gateway for the modulation of AMPK and its metabolic effects in skeletal muscle cells. ABSTRACT Methotrexate,
Klemen Dolinar +9 more
wiley +1 more source
Ligand binding and circular permutation modify residue interaction network in DHFR.
PLoS Computational Biology, 2007 Residue interaction networks and loop motions are important for catalysis in dihydrofolate reductase (DHFR). Here, we investigate the effects of ligand binding and chain connectivity on network communication in DHFR.
Zengjian Hu +6 more
doaj +1 more source
Intermittent preventive treatment of malaria provides substantial protection against malaria in children already protected by an insecticide-treated bednet in Burkina Faso: a randomised, double-blind, placebo-controlled trial. [PDF]
, 2011 BACKGROUND: Intermittent preventive treatment of malaria in children (IPTc) is a promising new approach to the control of malaria in areas of seasonal malaria transmission but it is not known if IPTc adds to the protection provided by an insecticide ...
Chandramohan, Daniel +17 more
core +3 more sources
Non-antibiotic Small-Molecule Regulation of DHFR-Based Destabilizing Domains In Vivo
Molecular Therapy: Methods & Clinical Development, 2019 The E. coli dihydrofolate reductase (DHFR) destabilizing domain (DD), which shows promise as a biologic tool and potential gene therapy approach, can be utilized to achieve spatial and temporal control of protein abundance in vivo simply by ...
Hui Peng +11 more
doaj +1 more source
Nucleic Acids Research, 1996 We have constructed expression vectors for Chinese hamster ovary (CHO) cells that produce both selectable marker and recombinant cDNA from a single primary transcript via differential splicing.
Brian K. Lucas +5 more
semanticscholar +1 more source
Life cycle studies of the hexose transporter of Plasmodium species and genetic validation of their essentiality [PDF]
, 2010 A Plasmodium falciparum hexose transporter (PfHT) has previously been shown to be a facilitative glucose and fructose transporter. Its expression in Xenopus laevis oocytes and the use of a glucose analogue inhibitor permitted chemical validation of PfHT ...
Doerig, C +6 more
core +2 more sources
Drug interactions may be important risk factors for methotrexate neurotoxicity, particularly in pediatric leukemia patients [PDF]
, 2016 Purpose: Methotrexate administration is associated with frequent adverse neurological events during treatment for childhood acute lymphoblastic leukemia.
Baird, Susan F. +5 more
core +2 more sources
The mitochondrial receptor complex [PDF]
, 1993 The receptor complex in the mitochondrial outer membrane, which consists of at least seven different proteins, is responsible for the recognition and translocation of cytosolically synthesized preproteins.
Alberts +55 more
core +4 more sources