Results 121 to 130 of about 40,426 (262)

Computational analysis of binding between malarial dihydrofolate reductases and anti-folates

Malaria Journal, 2010
Background Plasmodium falciparum readily develops resistance to the anti-folates pyrimethamine and proguanil via a characteristic set of mutations in the dihydrofolate reductase (PfDHFR) gene that leads to reduced competitive drug binding at the enzyme's
White Nicholas J, Day Nicholas PJ, Songtawee Napat, Theppabutr Sasikrit, Choowongkomon Kiattawee, Woodrow Charles J, Imwong Mallika   +6 more
doaj   +1 more source

The implication of dihydrofolate reductase and dihydropteroate synthetase gene mutations in modification of Plasmodium falciparum characteristics

Malaria Journal, 2007
Background The Plasmodium falciparum dihydrofolate reductase (DHFR) and dihydropteroate synthetase (DHPS) are enzymes of central importance in parasite metabolism.
Elbashir Mustafa I, Masuadi Emad M, Bygbjerg Ib C, Khalil Insaf F, Alifrangis Michael, A-Elbasit Ishraga E, Giha Hayder A   +6 more
doaj   +1 more source

Transgenic Plasmodium parasites stably expressing Plasmodium vivax dihydrofolate reductase-thymidylate synthase as in vitro and in vivo models for antifolate screening

Malaria Journal, 2011
Background Plasmodium vivax is the most prevalent cause of human malaria in tropical regions outside the African continent. The lack of a routine continuous in vitro culture of this parasite makes it difficult to develop specific drugs for this disease ...
Yuthavong Yongyuth, Srichairatanakool Somdet, Prommana Parichat, Uthaipibull Chairat, Somsak Voravuth, Kamchonwongpaisan Sumalee   +5 more
doaj   +1 more source

HBsAg真核表达载体的构建及其在CHO细胞中的表达

Zhongguo shiyan zhenduanxue, 2007
目的构建HBsAg真核表达载体,转染CHO细胞并检测其表达。方法通过PCR法扩增出乙肝表面抗原的S基因和dhfr基因,分别克隆到T载体上,然后分别将S基因和dhfr基因克隆到pCI载体上,将pCI载体的CMV启动子替换为hEF-1α启动子。对新构建的pEF1-αS-dhfr真核表达载体进行酶切和测序鉴定。用脂质体法转染CHO（dhfr-）细胞,并用ELISA法检测HBsAg表达。结果hEF-1α启动子、S基因和dhfr基因经测序与Genbank报道一致。真核表达载体pEF1-αS ...
张艳, 时成波, 郭丽, 范洪学
doaj  

Extraction of ursolic acid from Ocimum sanctum and synthesis of its novel derivatives: effects on extracellular homocysteine, dihydrofolate reductase activity and proliferation of HepG2 human hepatoma cells

Pteridines, 2013
The objective of the present study was to extract ursolic acid (UA) from Ocimum sanctum, to synthesize its bioactive derivatives, evaluate the anti-cancer effect of its derivatives and to establish the possible mechanism of action. In the present report,
Batra Apsara, Sastry V. Girija
doaj   +1 more source

Disease-linked mutations trigger exposure of a protein quality control degron in the DHFR protein [PDF]

, 2021
Caroline Kampmeyer, Sven Larsen-Ledet, Morten Rose Wagnkilde, Mathias Michelsen, Henriette K. M. Iversen, Sofie V. Nielsen, Søren Lindemose, A. Caregnato, Tommer Ravid, Amelie Stein, Kaare Teilum, Kresten Lindorff‐Larsen, Rasmus Hartmann‐Petersen   +12 more
openalex   +1 more source

Alleviation of accelerated diabetic atherogenesis in STZ-treated apoE/NOX1 DKO mice, apoE−/−/tg-EC-DHFR mice, and by folic acid

Redox Biology
We and others have previously shown that uncoupling of endothelial nitric oxide synthase (eNOS) induces oxidative stress in diabetes, contributing to endothelial dysfunction.
Yixuan Zhang, Ji Youn Youn, Kai Huang, Yuhan Zhang, Hua Cai   +4 more
doaj   +1 more source

Screening of polymorphisms for MTHFR and DHFR genes in spina bifida children and their mothers [PDF]

, 2013
M. Z. Husna, I. Endom, Sulaiman S. Ibrahim, Nur Selvi, H. Fakhrurazi, R. Ohnmar Htwe, Y. Kanehaswari, Abdul Razik Abdul Halim, Sing‐Wai Wong, K. Subashini, O. Nur Syahira, S. Aishah   +11 more
openalex   +1 more source

Design, synthesis, in vitro and in silico studies of novel piperidine derived thiosemicarbazones as inhibitors of dihydrofolate reductase

Scientific Reports
Dihydrofolate reductase (DHFR), an essential enzyme in folate metabolism, presents a promising target for drug development against various diseases, including cancer and tuberculosis.
Hina Aftab, Saeed Ullah, Ajmal Khan, Mariya al-Rashida, Talha Islam, Kholood A. Dahlous, Saikh Mohammad, Hamdy Kashtoh, Ahmed Al-Harrasi, Zahid Shafiq   +9 more
doaj   +1 more source

Rapid gene-specific repair of cisplatin lesions at the human DUG/DHFR locus comprising the divergent upstream gene and dihydrofolate reductase gene during early G1 phase of the cell cycle assayed by using the exonucleolytic activity of T4 DNA polymerase. [PDF]

, 1994
Nicholas J. Rampino, Vilhelm A. Bohr
openalex   +1 more source