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Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers

ACS Synthetic Biology, 2022
Rhamnolipids (RLs) are biosurfactants with great economic significance that have been used extensively in multiple industries. Pseudomonas aeruginosa is a promising microorganism for sustainable RL production. However, current CTAB-MB based screening of RL-producing strains is time-consuming, labor-intensive, and unable to distinguish mono- and di-RL ...
Xu, Anming   +8 more
openaire   +2 more sources

Unveiling the mono-rhamnolipid and di-rhamnolipid mechanisms of action upon plasma membrane models

Journal of Colloid and Interface Science, 2022
Rhamnolipids (RLs) are biosurfactants with significant tensioactive and emulsifying properties. They are mainly composed by mono-RL and di-RL components. Although there are numerous studies concerning their molecular properties, information is scarce regarding the mechanisms by which each of the two components interacts with cell membranes.
Alessandra, Marega Motta   +5 more
openaire   +2 more sources

Synthesis of α-1,2- and α-1,3-linked di-rhamnolipids for biological studies

Carbohydrate Research, 2020
For a detailed examination of the interaction of rhamnose containing derivatives with recombinant horseshoe crab plasma lectin (rHPL), two di-rhamno-di-lipids (an α-1,2- and an α-1,3-linked) were synthesized via a new simple method. The N-iodosuccinimide/triflic acid mediated glycosylation of the methyl (R)-3-hydroxydecanoate with phenyl-1-thio ...
Fruzsina, Demeter   +5 more
openaire   +2 more sources

Comparison of mono‐rhamnolipids and di‐rhamnolipids on microbial enhanced oil recovery (MEOR) applications

Biotechnology Progress, 2020
Abstract Rhamnolipids (RMLs) have more effectiveness for specific uses according to their homologue proportions. Thus, the novelty of this work was to compare mono‐RMLs and di‐RMLs physicochemical properties on microbial enhanced oil recovery (MEOR) applications. For this, RML produced by three strains of
Vanessa A. L. Rocha   +6 more
openaire   +2 more sources

Di-rhamnolipid is a mosquito pupicidal metabolite from Pseudomonas fluorescens (VCRC B426)

Acta Tropica, 2015
Pseudomonas fluorescens Migula (VCRC B426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti. The mosquito pupicidal metabolite from P. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica ...
G, Prabakaran   +3 more
openaire   +2 more sources

Biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered Escherichia coli

Biotechnology Letters, 2017
To engineer Escherichia coli for the heterologous production of di-rhamnolipids, which are important biosurfactants but mainly produced by opportunistic pathogen Pseudomonas aeruginosa.The codon-optimized rhlAB and rhlC genes originating from P. aeruginosa and Burkholderia pseudomallei were combinatorially expressed in E.
Jin, Du   +3 more
openaire   +2 more sources

Antibiofilm effect of mono‐rhamnolipids and di‐rhamnolipids on carbon steel submitted to oil produced water

Biotechnology Progress, 2021
Abstract The objective of this study was to compare the potential of mono‐rhamnolipids (mono‐RML) and di‐rhamnolipids (di‐RML) against biofilm formation on carbon steel coupons submitted to oil produced water for 14 days. The antibiofilm effect of the RML on the coupons was analyzed by scanning electron microscopy
Vanessa Alves Lima Rocha   +8 more
openaire   +2 more sources

Influence of di-rhamnolipids on the enzymatic hydrolysis of steam-pretreated eucalyptus wastes

Industrial Crops and Products, 2019
Abstract In this present work, rhamnolipids produced from Pseudomonas aeruginosa TGC07 were evaluated as biosurfactants for enzymatic hydrolysis of steam pretreated (not delignified) eucalyptus wastes comparatively to the synthetic surfactant Triton X-100.
Ricardo Mesquita   +4 more
openaire   +1 more source

Enhanced di-rhamnolipid production with an indigenous isolate Pseudomonas aeruginosa J16

Process Biochemistry, 2008
Abstract A newly isolated indigenous strain Pseudomonas aeruginosa J16 was able to produce a glycolipid biosurfactant (i.e., rhamnolipid). Analyses with 13C NMR and mass spectrometry show that the biosurfactant consisted mainly of mono-rhamnolipid (RL1) and di-rhamnolipid (RL2).
Yu-Hong Wei   +3 more
openaire   +1 more source

Increase proportion of di‐rhamnolipids biosynthesized from Pseudomonas aeruginosa and evaluation of relationship between activity and di‐rhamnolipids proportion

Journal of Surfactants and Detergents
AbstractRhamnolipids, simply divided into mono‐rhamnolipids and di‐rhamnolipids, their physicochemical properties are structure‐dependent. This study explored to increase the proportion of di‐rhamnolipids biosynthesized from Pseudomonas aeruginosa and evaluate the relationship between activity and di‐rhamnolipids proportions. P.
Yuting Wu   +4 more
openaire   +1 more source

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