Results 191 to 200 of about 1,002,484 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

P650: Myotonic dystrophy type 1 genetic testing in over 30,000 patients: Does size matter as patients get older?

Genetics in Medicine Open
Zoe Powis, Khalida Liaquat, Marc Meservey, Judy Louie, Sat Dev Batish   +4 more
doaj   +1 more source

Establishing an EU-compliant diagnostic facility for infectious diseases under war conditions in Poltava, Ukraine. [PDF]

Front Public Health
Zelinskyy G, Koval T, Schrammel U, Elsner C, Ackermann J, Marchenko O, Sydorenko M, Shlykova O, Izmailova O, Kabaliei A, Dziobaka J, Verhasselt HL, Buer J, Heintschel von Heinegg E, Trilling M, Kaydashev I, Dittmer U.   +16 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Patients' Perspectives on the Implementation of AI in Radiological Diagnostics: Focus Group Study. [PDF]

J Med Internet Res
Karger CR.
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue. [PDF]

Biomed Opt Express
Kumar S, Singh R, Schena E, Min R, Marques C.   +4 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Correction: Bulbul, C.B.; Yakistiran, B. First Trimester Triglyceride-Glucose Index and Lipid Profile as Predictive Factors in the Diagnosis of Late-Onset Preeclampsia: Can We Prevent It? <i>Diagnostics</i> 2025, <i>15</i>, 3141. [PDF]

Diagnostics (Basel)
Bulbul CB, Yakistiran B.
europepmc   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source