Results 81 to 90 of about 423,351 (316)

LED-pump-X-ray-multiprobe crystallography for sub-second timescales

open access: yesCommunications Chemistry, 2022
Time-resolved single-crystal X-ray diffraction experiments largely focus on timescales shorter than microseconds, whereby slower population dynamics are missed.
Lauren E. Hatcher   +12 more
doaj   +1 more source

Single Cell Cryo-Soft X-ray Tomography Shows That Each Chlamydia Trachomatis Inclusion Is a Unique Community of Bacteria

open access: yesLife, 2021
Chlamydiae are strict intracellular pathogens residing within a specialised membrane-bound compartment called the inclusion. Therefore, each infected cell can, be considered as a single entity where bacteria form a community within the inclusion.
Patrick Phillips   +8 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Fast-feedback for unattended data collection at Diamond Light Source [PDF]

open access: yesStructural Dynamics
Diamond Light Source's I03 beamline now spends most of its time in "Unattended Data Collection" mode. To maximise throughput, it is critical that as little time is spent waiting for x-ray centering as possible. In this talk, I will describe the GPU-based
Nicholas Devenish   +2 more
doaj   +1 more source

Columnar Cvd Diamond Growth Structure On Irregular Surface Substrates [PDF]

open access: yes, 2015
Columnar grain structure is always observed in CVD-diamond growth and is an important parameter to identify the morphology of thin and thick films. Structure defects, aspects of onset nucleation and film growth mechanisms can also be related to columnar ...
Baranauskas V.   +5 more
core   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

SuRVoS 2: Accelerating Annotation and Segmentation for Large Volumetric Bioimage Workflows Across Modalities and Scales

open access: yesFrontiers in Cell and Developmental Biology, 2022
As sample preparation and imaging techniques have expanded and improved to include a variety of options for larger sized and numbers of samples, the bottleneck in volumetric imaging is now data analysis.
Avery Pennington   +7 more
doaj   +1 more source

Diamond components with integrated abrasion sensor for tribological applications [PDF]

open access: yes, 2022
S.991-995Tribological components made from CVD diamond are commonly used for protection against abrasion in rough environments. Such components can be used e.g.
Obloh, H.   +5 more
core   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

open access: yesFEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

open access: yesFEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno   +3 more
wiley   +1 more source

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