Comparative analysis of discrete exosome fractions obtained by differential centrifugation [PDF]
Journal of Extracellular Vesicles, 2014 Background: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ in size, density and composition. The standard isolation method for exosomes is centrifugation of fluid samples, typically at 100,000×g or above. Knowledge
Dennis K. Jeppesen +8 more
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Guidelines for an optimized differential centrifugation of cells [PDF]
Biochemistry and Biophysics Reports, 2023 Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols.
Mohammad Sharifian Gh., Fatemeh Norouzi
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Pentapartite fractionation of particles in oral fluids by differential centrifugation [PDF]
Scientific Reports, 2021 Oral fluids (OFs) contain small extracellular vesicles (sEVs or exosomes) that carry disease-associated diagnostic molecules. However, cells generate extracellular vesicles (EVs) other than sEVs, so the EV population is quite heterogeneous.
Chiho Hiraga +9 more
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Preparation of Crude Subcellular Fractions by Differential Centrifugation [PDF]
The Scientific World Journal, 2002 The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient.
John Graham
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Selective loss of microvesicles is a major issue of the differential centrifugation isolation protocols [PDF]
Scientific Reports, 2021 Microvesicles (MVs) are large extracellular vesicles differing in size, cargo and composition that share a common mechanism of release from the cells through the direct outward budding of the plasma membrane.
Annamaria Nigro +6 more
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Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements [PDF]
J Vis Exp, 2017 Mitochondria are involved in cellular energy metabolism and use oxygen to produce energy in the form of adenosine triphosphate (ATP). Differential centrifugation at low- and high-speed is commonly used to isolate mitochondria from tissues and cultured ...
Siamak Djafarzadeh +1 more
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Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol [PDF]
Sci Rep, 2015 Exosomes, small (40–100 nm) extracellular membranous vesicles, attract enormous research interest because they are carriers of disease markers and a prospective delivery system for therapeutic agents.
M. A. Livshits +7 more
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Reproducibility of Differential Centrifugation Experiments in Tissue Fractionation [PDF]
Nature, 1953 DURING the past few years, differential centrifugation of tissue homogenates has become one of the major techniques of cytology and biochemistry alike. Most of those who have applied this new technique describe the conditions of centrifugation by mentioning only the duration of operation and the centrifugal field prevailing in the middle (or at the ...
C. De Duve, J. Berthet
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Size-isolation of ultrasound-mediated phase change perfluorocarbon droplets using differential centrifugation [PDF]
J Acoust Soc Am, 2016 Perfluorocarbon droplets that are capable of an ultrasound-mediated phase transition have applications in diagnostic and therapeutic ultrasound. Techniques to modify the droplet size distribution are of interest because of the size-dependent acoustic ...
Karla P. Mercado +5 more
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The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens [PDF]
PLoS Negl Trop Dis, 2019 Background Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-secretory products are necessary for downstream studies and application development.
Chelsea N. Davis +6 more
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