Results 91 to 100 of about 2,730,068 (332)

Exosome isolation from distinct biofluids using precipitation and column-based approaches. [PDF]

PLoS ONE, 2018
The potential of exosomes as biomarker resources for diagnostics, prognostics and even for therapeutics is an area of intense research. Despite the various approaches available, there is no consensus with respect to the best methodology for isolating ...
Tânia Soares Martins, José Catita, Ilka Martins Rosa, Ilka Martins Rosa, Odete A B da Cruz E Silva, Ana Gabriela Henriques   +5 more
doaj   +1 more source

Increased Expression of Cell-Cell Signaling Genes by Stimulated Mononuclear Leukocytes in Patients with Previous Atherothrombotic Stroke A Whole Genome Expression Profile Study [PDF]

, 2009
Background/Aims: Inflammation plays an important role in atherosclerosis and stroke. Acute infections are recognized as trigger factors for ischemic stroke. Methods: In this whole genome expression profile study of 15 patients and 15 control subjects, we
Grau, Armin J., Grond-Ginsbach, Caspar, Hergenhahn, Manfred, Hollstein, Monika, Honold, Cornelius, Horstmann, Solveig, Hummel, Manuela, Knyazev, Yuri, Mansmann, Ulrich, Pfleger, Karin, Wagner, Simone, Weninger, Annette, Wiest, Tina   +12 more
core   +1 more source

Neutrophil plasma membranes. I. High-yield purification of human neutrophil plasma membrane vesicles by nitrogen cavitation and differential centrifugation

Journal of Cell Biology, 1980
Neutrophil chemotaxis, phagocytosis, and oxygen-dependent microbicidal activity are initiated by interactions of stimuli with the plasma membrane.
M. Klempner, R. Mikkelsen, D. Corfman, J. André‐Schwartz   +3 more
semanticscholar   +1 more source

Cytospin Centrifuge in Differential Counts of Milk Somatic Cells

Journal of Dairy Science, 1982
A procedure was developed for a cytospin centrifuge to concentrate cells from milk onto microscope slides in a circle with 6 mm diameter. Differential somatic cell counts with cytospin were compared to the conventional hand smearing technique to ascertain variation in differential counts.
Max Paape, A.M. Dulin, B.T. Weinland
openaire   +3 more sources

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

FEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner, Juthamas Khamseekaew, M. Gregor Madej, Linda Geiges, Bardha Azemi, Christine Ziegler, Christoph Korbmacher, Alexandr V. Ilyaskin   +7 more
wiley   +1 more source

Methods for extracellular vesicles isolation in a hospital setting

Frontiers in Immunology, 2015
The research in extracellular vesicles (EVs) has been rising during the last decade. However, there is no clear consensus on the most accurate protocol to isolate and analyze them.
Matías eSáenz-Cuesta, Matías eSáenz-Cuesta, Ander eArbelaiz, Ander eArbelaiz, Amaia eOregi, Haritz eIrizar, Haritz eIrizar, Iñaki eOsorio-Querejeta, Iñaki eOsorio-Querejeta, Maider eMuñoz-Culla, Maider eMuñoz-Culla, Jesús M Bañales, Jesús M Bañales, Jesús M Bañales, Jesús M Bañales, Jesús M Bañales, Juan M Falcon-Perez, Juan M Falcon-Perez, Juan M Falcon-Perez, Javier eOlascoaga, Javier eOlascoaga, David eOtaegui, David eOtaegui   +22 more
doaj   +1 more source

Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. [PDF]

, 2017
ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, is frequently mutated in cancer. Deficiency in its homolog ARID1B is synthetically lethal with ARID1A mutation.
Amaral, Maria Luisa, Benner, Christopher, Hargreaves, Diana C, Kelso, Timothy WR, Porter, Devin K, Shokhirev, Maxim N   +5 more
core   +1 more source

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

FEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang, Frans Bianchi, Geert van den Bogaart   +2 more
wiley   +1 more source

Ion-exchange chromatography purification of extracellular vesicles

BioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović, Bojana Milutinović, Sanja Goč, Ninoslav Mitić, Miroslava Janković   +4 more
doaj   +1 more source

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source