Results 101 to 110 of about 2,730,068 (332)

Chloroplast DNA Extraction from Herbaceous and Woody Plants for Direct Restriction Fragment Length Polymorphism Analysis

BioTechniques, 2000
The technique described here is a fast and simple method of extracting chloroplast DNA (cpDNA). It overcomes the need for differential centrifugation using density gradients. The leaves do not have to be kept in the dark and lyophilized before extraction,
C. Mariac, P. Trouslot, C. Poteaux, G. Bezançon, J.-F. Renno   +4 more
doaj   +1 more source

Brucella NyxA and NyxB dimerization enhances effector function during infection

FEBS Letters, EarlyView.
Brucella abortus thrives inside cells thanks to the translocation of effector proteins that fine‐tune cellular functions. NyxA and NyxB are two effectors that destabilize the nucleolar localization of their host target, SENP3. We show that the Nyx proteins directly interact with each other and that their dimerization is essential for their function ...
Lison Cancade‐Veyre, Arthur Louche, Francine C. A. Gérard, Laurent Terradot, Suzana P. Salcedo   +4 more
wiley   +1 more source

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

FEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg, Laura De Cnop, Bernd Nidetzky   +2 more
wiley   +1 more source

Oxygen-dependent expression of the erythropoietin gene in rat hepatocytes in vitro [PDF]

, 1993
Since in juvenile rats the liver is the predominant site of erythropoietin (EPO) gene expression, we have used primary cultures of juvenile rat hepatocytes to establish and in vitro system for investigation of oxygen-dependent EPO formation.
Eckardt, Kai-Uwe, Kurtz, Armin, Pugh, C. W., Ratcliffe, P. J.   +3 more
core   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Konsentrasi PEG 6000 dan Senyawa Aditif Buffer Fosfat yang Diperlukan dalam Pemurnian Soybean Mosaic Virus

Jurnal Perlindungan Tanaman Indonesia, 1995
The objective of study was to determine the concentration of PEG 6000 for precipitation of virus particles, and additive substance added to the resuspension buffer, during the purification of Soybean Mosaic Virus isolated from Yogyakarta.
YB Sumardiyono, Susamto Somowiyarjo, Wuye Ria Andayani   +2 more
doaj   +1 more source

Investigating the effect of target of rapamycin kinase inhibition on the Chlamydomonas reinhardtii phosphoproteome: from known homologs to new targets [PDF]

, 2019
Recuperado de: https://www.biorxiv.org/content/10.1101/310102v1Target of rapamycin (TOR) kinase is a conserved regulator of cell growth whose activity is modulated in response to nutrients, energy and stress.
Couso Liáñez, Inmaculada Concepción, Crespo González, José Luis, Hicks, Leslie, McConnell, Evan W., Perrine, Zoee, Umen, James G., Werth, Emily G.   +6 more
core   +1 more source

P‐glycoprotein modulates the fluidity gradient of the plasma membrane of multidrug resistant CHO cells

FEBS Letters, EarlyView.
To explore the impact of the overexpression of the multidrug‐transporter P‐glycoprotein (ABCB1) on membrane fluidity, we compared the transversal gradient of mobility and microviscosity in plasma membranes of drug‐sensitive Chinese hamster ovary cells (AuxB1) and their multidrug‐resistant derivatives (B30) using the fluorescent n‐(9‐anthroyloxy) fatty ...
Roger Busche, John R. Riordan, Burkhard Tümmler   +2 more
wiley   +1 more source

Calsequestrins in skeletal and cardiac muscle from adult Danio rerio [PDF]

, 2015
Calsequestrin (Casq) is a high capacity, low affinity Ca2+-binding protein, critical for Ca2+-buffering in cardiac and skeletal muscle sarcoplasmic reticulum. All vertebrates have multiple genes encoding for different Casq isoforms.
Argenton, Francesco, Furlan, Sandra, Mosole, Simone, Murgia, Marta, Nagaraj, Nagarjuna, Nori, Alessandra, Volpe, Pompeo   +6 more
core   +1 more source

A coiled‐coil domain triggers oligomerization of MmpL10, the mycobacterial transporter of trehalose polyphleate precursor

FEBS Letters, EarlyView.
Most MmpLs transport lipids and glycolipids of the complex mycomembrane of mycobacteria. This study shows that MmpL10 from Mycobacterium smegmatis, the transporter of the trehalose polyphosphate precursor, harbors a coiled‐coil‐like extension. Biochemical and electron microscopy studies demonstrate that the coiled‐coil enables MmpL10 to trimerize.
Julie Couston, Jérôme Feuillard, Aurélie Ancelin, Joséphine Lai‐Kee‐Him, Konstantin Brodolin, Christian Chalut, Pontus Gourdon, Mickaël Blaise   +7 more
wiley   +1 more source