FRACTIONATION OF MAMMALIAN LIVER CELLS BY DIFFERENTIAL CENTRIFUGATION [PDF]
Journal of Experimental Medicine, 1946 1. A method is described whereby the major components of liver suspensions are segregated according to size into three main fractions: (a) a large granule fraction composed of elements approximately 0.5 to 2 µ in diameter; (b) a microsome fraction ...
Albert Claude
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Isolating Brain Mitochondria by Differential Centrifugation
Bio-Protocol, 2016 In addition to methods aimed at the study of mitochondrial function in-situ, a full understanding of mitochondrial function requires their purification from cells or tissues under specific physiological or pathological conditions.
Ignacio Amigo +2 more
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Purification of Synechococcus lividus by Equilibrium Centrifugation and Its Synchronization by Differential Centrifugation [PDF]
Journal of Bacteriology, 1973 A culture of the thermophilic, unicellular, blue-green alga Synechococcus lividus was freed from two thermophilic bacteria by use of equilibrium (isopycnic) centrifugation in Ficoll density gradients. After removal of the bacteria, the alga would grow only on agar plates in a high carbon dioxide atmosphere ...
Thomas O. Sitz, Robert R. Schmidt
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THE CONTRIBUTIONS OF DIFFERENTIAL CENTRIFUGATION TO THE INTRACELLULAR LOCALIZATION OF ENZYMES [PDF]
Journal of Histochemistry & Cytochemistry, 1957 The most essential contribution of differential centrifugation to our understanding of the cell is so commonplace now that we may overlook it . The intracellular localization of chemical substances, revealed by this technique above all others, implies an inter-dependence of subcellular units. Almost from the birth of cytology, the biologist has assumed
Alex B. Novikoff, Estelle Podber
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BioTechniques, 2012 When separating two species with similar densities but differing sedimentation velocities (because of differences in size), centrifugal elutriation is generally the method of choice.
Jinwang Tan +3 more
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Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation [PDF]
STAR ProtocolsSummary: The mitochondrial genome (mitogenome) is a vital tool in molecular phylogenetics, evolutionary biology, and population genetics. Here, we present a protocol for extracting mitochondrial DNA from shrimp (Macrobrachium rosenbergii) and prawn ...
Dipta Chandra Pal +1 more
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Differential Centrifugation in Culture and Differentiation of Rat Neural Stem Cells [PDF]
Cell Mol Neurobiol, 2008 (1) The study of neural stem cells (NSC) has attracted much attention in recent years because of their therapeutic potential. However, the problem in culture and differentiation of NSC was how to obtain single cell suspension that preserves the function of NSC, and remove the debris caused by mechanical dissociation.
S. Ye +5 more
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Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation [PDF]
Biophys RepThe endoplasmic reticulum (ER) is an essential component of the endomembrane system in eukaryotes and plays a crucial role in protein and lipid synthesis, as well as the maintenance of calcium homeostasis. Morphologically, the ER is composed primarily of sheets and tubules.
Yiduo Liu, Junjie Hu, Bing Yan
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Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation [PDF]
Methods Mol Biol, 2007 The number of proteins found associated with cell-cell adhesion substructures is growing rapidly. Based on potential protein-protein interactions, complex protein networks at cell-cell contacts can be modeled. Traditional studies to examine protein-protein interactions include co-immunoprecipitation or pull-down experiments of tagged proteins.
Roger Vogelmann, W. James Nelson
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Low-Vacuum Filtration as an Alternative Extracellular Vesicle Concentration Method: A Comparison with Ultracentrifugation and Differential Centrifugation [PDF]
Pharmaceutics, 2020 Recent years have brought great focus on the development of drug delivery systems based on extracellular vesicles (EVs). Considering the possible applications of EVs as drug carriers, the isolation process is a crucial step.
A. Drożdż +7 more
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