Comparative analysis of discrete exosome fractions obtained by differential centrifugation [PDF]
Journal of Extracellular Vesicles, 2014 Background: Cells release a mixture of extracellular vesicles, amongst these exosomes, that differ in size, density and composition. The standard isolation method for exosomes is centrifugation of fluid samples, typically at 100,000×g or above. Knowledge
Dennis K. Jeppesen +8 more
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Guidelines for an optimized differential centrifugation of cells [PDF]
Biochemistry and Biophysics Reports, 2023 Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols.
Mohammad Sharifian Gh., Fatemeh Norouzi
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Selective loss of microvesicles is a major issue of the differential centrifugation isolation protocols [PDF]
Scientific Reports, 2021 Microvesicles (MVs) are large extracellular vesicles differing in size, cargo and composition that share a common mechanism of release from the cells through the direct outward budding of the plasma membrane.
Annamaria Nigro +6 more
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An Improved Method to Enrich Large Extracellular Vesicles Derived from Giardia intestinalis through Differential Centrifugation [PDF]
Life, 2023 Giardia intestinalis is a flagellated unicellular protozoan that colonizes the small intestine, causing the diarrheal disease called giardiasis. The production of extracellular vesicles (EVs) by G. intestinalis and the role of these EVs in the parasite’s
Abel Sana +5 more
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Pentapartite fractionation of particles in oral fluids by differential centrifugation [PDF]
Scientific Reports, 2021 Oral fluids (OFs) contain small extracellular vesicles (sEVs or exosomes) that carry disease-associated diagnostic molecules. However, cells generate extracellular vesicles (EVs) other than sEVs, so the EV population is quite heterogeneous.
Chiho Hiraga +9 more
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A Reproducible Protocol for the Isolation of Malaria-Derived Extracellular Vesicles by Differential Centrifugation [PDF]
Methods and ProtocolsOver the last few decades, malaria-derived extracellular vesicles (EVs) have gained increasing interest due to their role in disease pathophysiology and parasite biology.
Tosin Opadokun, Petra Rohrbach
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Isolating Liver Mitochondria by Differential Centrifugation
Bio-Protocol, 2016 In addition to methods aimed at the study of mitochondrial function in-situ, a full understanding of mitochondrial function requires their purification from cells or tissues under specific physiological or pathological conditions.
Ignacio Amigo +2 more
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Isolating Brain Mitochondria by Differential Centrifugation
Bio-Protocol, 2016 In addition to methods aimed at the study of mitochondrial function in-situ, a full understanding of mitochondrial function requires their purification from cells or tissues under specific physiological or pathological conditions.
Ignacio Amigo +2 more
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Preparation of Crude Subcellular Fractions by Differential Centrifugation [PDF]
The Scientific World Journal, 2002 The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient.
John Graham
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Differential Centrifugation to Enrich Bacterial Ribonucleoprotein Bodies (BR bodies) from Caulobacter crescentus [PDF]
STAR Protocols, 2020 Summary: Bacterial RNP bodies (BR bodies) contain the mRNA decay machinery, but the collection of associated RNAs and proteins are poorly defined. Here, we present a protocol for the rapid differential centrifugation-based enrichment of BR bodies from ...
Nisansala S. Muthunayake +2 more
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