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Subcellular fractionation by differential centrifugation for mitochondrial studies
In addition to fluorescence microscopy, the subcellular fractionation of eukaryotic cells remains one of the central methods for the basic characterization of proteins. Here we describe an optimized procedure for the subcellular fractionation of yeast cells, specifically for mitochondrial studies.Conny, Steiert +3 more
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Mouse alveolar surfactant: Characterization of subtypes prepared by differential centrifugation
Lipids, 1993AbstractTo characterize the properties of alveolar surfactant susbfractions obtained from mouse lung by differential centrifugation, lavage fluid, following a preliminary centrifugation at 140 × g for 5 min to yield a cellular pellet (Pc), was sequentially cetrifuged at 10,000 ×g for 30 min, 60,000 ×g for 60 min and 100,000 ×g for 15 h; and the ...
M, Oulton +3 more
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Analytical Biochemistry, 1998
A quick subcellular fractionation procedure using differential centrifugation, which is applicable to isolated and cultured cells, is presented. This technique was developed for studying the subcellular localization of phosphorylated proteins in isolated liver cells after various stimuli, but is also applicable to many other situations.
M, Bronfman, G, Loyola, C S, Koenig
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A quick subcellular fractionation procedure using differential centrifugation, which is applicable to isolated and cultured cells, is presented. This technique was developed for studying the subcellular localization of phosphorylated proteins in isolated liver cells after various stimuli, but is also applicable to many other situations.
M, Bronfman, G, Loyola, C S, Koenig
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Analytical Differential Centrifugation as a Precise Biophysical Tool
Separation Science and Technology, 1981Abstract Anderson (I) introduced a novel procedure to enable the ready acquisition of the complement of discrete sedimentation coefficients present in a mixture. This was developed as an aid in providing rational separations in zonal centrifuges, and has been applied over large molecular weight ranges (2).
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Differential centrifugation of bovine parathyroid tissue
Biochimica et Biophysica Acta, 1956M V, L'HEUREUX, P, MELIUS
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Isolation of Mitochondria from Tissues and Cells by Differential Centrifugation
Current Protocols in Cell Biology, 1999AbstractDifferential centrifugation is used to prepare a “heavy” mitochondrial fraction from liver. These mitochondria are relatively pure, highly coupled, and suitable for respiratory studies. This unit presents protocols for isolation of beef heart mitochondria (also suitable for respiratory studies), skeletal muscle mitochondria, and mitochondria ...
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[Hydrodynamic study of a centrifugal differential-contact extractor].
Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology, 1985Centrifugal extractors of the differential-contact type are widely used all over the world in production of antibiotics. Lately, new extractors were developed by Hitachi (Japan). Model E is of a special interest. In the present study the values of the working zone of the Hitachi extractor, EP-02 Ultrex were determined by the pressure levels and the ...
V R, Katrush +4 more
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DIFFERENTIAL CENTRIFUGALIZATION
Journal of the American Medical Association, 1922openaire +1 more source
Preparation of discoid washed platelets by differential centrifugation
Clinica Chimica Acta, 1998Y, Hayashi +3 more
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