Behavior of sterols of insect muscle during homogenization and differential centrifugation
Labeled sterol introduced in vitro into a homogenate of insect muscle became distributed in the various subcellular fractions in a manner similar to that obtained by fractionation of muscle of insects to which labeled sterol had been administered in vivo.
W.R. Roeske, R.B. Clayton
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Immunoassay of digoxin by differential centrifugation. [PDF]
Abstract We describe an immunoassay of digoxin that exploits the differential sedimentation rate between two types of latex particles in a microcentrifugal analyzer. Conventional polystyrene latex particles (relative density = 1.05), sensitized with antibody, are first suspended with the sample and then mixed with digoxin-sensitized ...
Brian Lentrichia+4 more
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An Improved Method to Enrich Large Extracellular Vesicles Derived from Giardia intestinalis through Differential Centrifugation [PDF]
Giardia intestinalis is a flagellated unicellular protozoan that colonizes the small intestine, causing the diarrheal disease called giardiasis. The production of extracellular vesicles (EVs) by G. intestinalis and the role of these EVs in the parasite’s
Abel Sana+5 more
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Isolating Liver Mitochondria by Differential Centrifugation
In addition to methods aimed at the study of mitochondrial function in-situ, a full understanding of mitochondrial function requires their purification from cells or tissues under specific physiological or pathological conditions.
Ignacio Amigo+2 more
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A Reproducible Protocol for the Isolation of Malaria-Derived Extracellular Vesicles by Differential Centrifugation [PDF]
Over the last few decades, malaria-derived extracellular vesicles (EVs) have gained increasing interest due to their role in disease pathophysiology and parasite biology.
Tosin Opadokun, Petra Rohrbach
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Corrigendum: Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol [PDF]
Scientific Reports 5: Article number: 17319; published online: 30 November 2015; updated: 29 March 2016 The original version of this Article contained a typographical error in the spelling of the author Mikhail A. Livshits, which was incorrectly given as
M. Livshits+7 more
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Purification of Murine Gammaherpesvirus 68 With Use of Differential Centrifugation [PDF]
The method for separation of viral particles in a concentrated form from the environment is called virus purification. Viruses are required to be purified for a range of studies in which it is necessary to distinguish the properties or structure of a ...
Hodoši R.+2 more
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BIOCHEMICAL PROPERTIES OF FRACTIONS OBTAINED FROM RAT ANTERIOR PITUITARY GLANDS BY DIFFERENTIAL CENTRIFUGATION1 [PDF]
THE biochemistry of the nuclear, mitochondrial, microsomal and supernatant fractions isolated by differential centrifugation from rat and mouse liver has been studied extensively during the past few years.
W. H. McShan, R Rozich, Roland K. Meyer
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Comparative Studies of the Hill Activity of Differentially Centrifuged Chloroplast Fractions [PDF]
41. WEISS, S. P. AND E. P. KENNEDY. 1956. The enzymatic synthesis of triglycerides. J. Am. Chem. Soc. 78: 3550. 42. WEISS, S. P., S. WV. SMITH, AND E. P. KENNEDY. 1956. Net synthesis of lecithin in an isolated enzyme system. Nature 178: 594. 43. WINTERMANS, J. F. G. M. 1960.
Milton J. Becker+2 more
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Differential centrifugation–based biochemical fractionation of the Drosophila adult CNS [PDF]
Drosophila is widely used as a genetic model in questions of development, cellular function and disease. Genetic screens in flies have proven to be incredibly powerful in identifying crucial components for synapse formation and function, particularly in the case of the presynaptic release machinery.
H. Depner+4 more
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