Results 291 to 300 of about 2,730,068 (332)
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Analytical Differential Centrifugation as a Precise Biophysical Tool
Separation Science and Technology, 1981Abstract Anderson (I) introduced a novel procedure to enable the ready acquisition of the complement of discrete sedimentation coefficients present in a mixture. This was developed as an aid in providing rational separations in zonal centrifuges, and has been applied over large molecular weight ranges (2).
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Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Journal of Visualized Experiments, 2018In sexual reproduction, a male gamete or sperm cell fuses with a female gamete to bring about fertilization. However, a large number of sperm cells with fertilizing ability are required to interact with a female gamete to ensure fertilization. As such, the fertilizing ability of individual sperm cells is critical for successful reproduction.
Zachery Ryan Jarrell +2 more
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Acta Pharmacologica et Toxicologica, 2009
Microsomes were prepared from the liver, kidney and lung of phenobarbital or 20-methylcholanthrene treated and control rats with the conventional ultracentrifugation and calcium aggregation methods.
A. Aitio, H. Vainio
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Microsomes were prepared from the liver, kidney and lung of phenobarbital or 20-methylcholanthrene treated and control rats with the conventional ultracentrifugation and calcium aggregation methods.
A. Aitio, H. Vainio
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Mouse alveolar surfactant: Characterization of subtypes prepared by differential centrifugation
Lipids, 1993AbstractTo characterize the properties of alveolar surfactant susbfractions obtained from mouse lung by differential centrifugation, lavage fluid, following a preliminary centrifugation at 140 × g for 5 min to yield a cellular pellet (Pc), was sequentially cetrifuged at 10,000 ×g for 30 min, 60,000 ×g for 60 min and 100,000 ×g for 15 h; and the ...
M. Oulton +3 more
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Subcellular fractionation by differential centrifugation for mitochondrial studies
In addition to fluorescence microscopy, the subcellular fractionation of eukaryotic cells remains one of the central methods for the basic characterization of proteins. Here we describe an optimized procedure for the subcellular fractionation of yeast cells, specifically for mitochondrial studies.Conny, Steiert +3 more
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American Review of Respiratory Disease, 1982
Mononuclear phagocytes are functionally heterogeneous. To study the relationship of the heterogeneous populations of macrophages from the lung, alveolar macrophages from Syrian hamsters that had been immunized and rechallenged intratracheally with ...
B. Zwilling, L. B. Campolito, N. Reiches
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Mononuclear phagocytes are functionally heterogeneous. To study the relationship of the heterogeneous populations of macrophages from the lung, alveolar macrophages from Syrian hamsters that had been immunized and rechallenged intratracheally with ...
B. Zwilling, L. B. Campolito, N. Reiches
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Differential particle size distributions from centrifugation experiments
Journal of Colloid and Interface Science, 1984Abstract Differential size distributions obtained with the Joyce—Loebl disk centrifuge are volume distributions (with linear size increment). Sedimentation field flow fractionation yields number distributions when performed at constant rotor speed, or volume distributions when the rotor speed is allowed to decay exponentially.
Simon C Haseler, Hans Coll
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Separation of microtubule populations in rat brain homogenates by differential centrifugation
Brain Research, 1986Subcellular fractions were separated from rat homogenates at 600 g for 5 min (P1), 15,000 g for 10 min (P2), 48,000 g for 60 min (P3), 100,000 g for 150 min (P4) and a final supernatant (S). They contained 5.0, 14.3, 14.7, 24.7 and 41.3% of the total tubulin in the homogenate measured by colchicine binding.
Francisco Bertini, Luis A. Lopez
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Differential centrifugation of bovine parathyroid tissue
Biochimica et Biophysica Acta, 1956Maurice V. L'Heureux, Paul Melius
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Differential centrifugation of leucocytes and platelets applied to 111In-leucocyte labelling
Nuclear Medicine Communications, 1986Platelet contamination of 111In-leucocytes can be minimized by centrifugation of leucocyte-rich plasma before labelling. The differential recovery of leucocytes and platelets from centrifugation depends on various biological and physical factors. Experimental measurements have been made of the effect of some of these factors.
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