Results 31 to 40 of about 273,854 (260)

Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage

open access: yesScientific Reports, 2022
The discovery and utility of clinically relevant circulating biomarkers depend on standardized methods that minimize preanalytical errors. Despite growing interest in studying extracellular vesicles (EVs) and cell-free messenger RNA (cf-mRNA) as ...
Hyun Ji Kim   +9 more
doaj   +1 more source

Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes

open access: yesMemorias do Instituto Oswaldo Cruz, 2015
Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison.
S Andrea Moreno, Mayerly Nava
doaj   +1 more source

Optimising PLGA-PEG Nanoparticle Size and Distribution for Enhanced Drug Targeting to the Inflamed Intestinal Barrier

open access: yesPharmaceutics, 2020
Oral nanomedicines are being investigated as an innovative strategy for targeted drug delivery to treat inflammatory bowel diseases. Preclinical studies have shown that nanoparticles (NPs) can preferentially penetrate inflamed intestinal tissues ...
Lauren J. Mohan   +3 more
doaj   +1 more source

Modern methods of granulometric analysis of dusted materials containing nanoparticles [PDF]

open access: yesФизико-химические аспекты изучения кластеров, наноструктур и наноматериалов, 2018
In the work the differential and integral distribution of particles of dolomite flour and wings of zinc-containing welsh furnaces by size was studied by the method of analytical disk centrifugation with X-ray detection.
A.Yu. Ataeva   +3 more
doaj   +1 more source

Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation

open access: yesActa Societatis Botanicorum Poloniae, 2012
A protocol is presented for low-cost and fast isolation of intact and pure chloroplasts from leaves of plants acclimated to low irradiance. The protocol is based on a differential centrifugation of cleared leaf homogenate and omits a centrifugation on ...
Magda Grabsztunowicz, Grzegorz Jackowski
doaj   +1 more source

Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads. [PDF]

open access: yesPLoS ONE, 2013
To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria needs to be precisely determined. The usual isolation methods such as differential centrifugation
Andras Franko   +10 more
doaj   +1 more source

Investigation of Integral and Differential Characteristics of Variatropic Structure Heavy Concretes by Ultrasonic Methods

open access: yesApplied Sciences, 2021
The article develops methods and methodology for experimental studies of centrifuged and vibro-centrifuged concrete products of annular cross-section. They assess the real variatropy of the structure and confirm the correctness of the accepted research ...
Levon R. Mailyan   +7 more
doaj   +1 more source

Purification of Murine Gammaherpesvirus 68 With Use of Differential Centrifugation

open access: yesEuropean Pharmaceutical Journal, 2021
The method for separation of viral particles in a concentrated form from the environment is called virus purification. Viruses are required to be purified for a range of studies in which it is necessary to distinguish the properties or structure of a ...
Hodoši R.   +2 more
doaj   +1 more source

Gamma-glutamyltransferase activity in exosomes as a potential marker for prostate cancer

open access: yesBMC Cancer, 2017
Background Exosomes or extracellular vesicles have the potential as a diagnostic marker for various diseases including cancer. In order to identify novel exosomal markers for prostate cancer (PC), we performed proteomic analysis of exosomes isolated from
Kyojiro Kawakami   +12 more
doaj   +1 more source

The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles

open access: yesJournal of Extracellular Vesicles, 2014
Background: Extracellular vesicles (EV), the collective term for vesicles released from cells, consist of vesicle species ranging in size from 30 nm to 5 µm in diameter.
Aleksander Cvjetkovic   +2 more
doaj   +1 more source

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