Results 41 to 50 of about 2,549,211 (293)

Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification. [PDF]

PLoS ONE, 2018
Isolated mitochondria are widely used to study the function of the organelle. Typically, mitochondria are prepared using differential centrifugation alone or in conjunction with density gradient ultracentrifugation.
Pin-Chao Liao, Istvan R Boldogh, Stephanie E Siegmund, Zachary Freyberg, Liza A Pon   +4 more
doaj   +1 more source

Isolation of Microvesicles from Human Circulating Neutrophils

Bio-Protocol, 2021
Neutrophil-derived microvesicles (NDMVs) are liberated by neutrophils upon cell activation by molecules. Once activated, neutrophils are primarily involved in acute inflammation; however, the microvesicles they produce are largely anti-inflammatory ...
Dong Zhan, Ellie McConachie, Steven Edwards, Helen Wright, Robert Moots, Sittisak Honsawek   +5 more
doaj   +1 more source

Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage

Scientific Reports, 2022
The discovery and utility of clinically relevant circulating biomarkers depend on standardized methods that minimize preanalytical errors. Despite growing interest in studying extracellular vesicles (EVs) and cell-free messenger RNA (cf-mRNA) as ...
Hyun Ji Kim, Matthew J. Rames, Samuel Tassi Yunga, Randall Armstrong, Mayu Morita, Anh T. P. Ngo, Owen J. T. McCarty, Fehmi Civitci, Terry K. Morgan, Thuy T. M. Ngo   +9 more
doaj   +1 more source

Modern methods of granulometric analysis of dusted materials containing nanoparticles [PDF]

Физико-химические аспекты изучения кластеров, наноструктур и наноматериалов, 2018
In the work the differential and integral distribution of particles of dolomite flour and wings of zinc-containing welsh furnaces by size was studied by the method of analytical disk centrifugation with X-ray detection.
A.Yu. Ataeva, G.I. Sverdlik, D.A. Kambolov, A.R. Ataev   +3 more
doaj   +1 more source

Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads. [PDF]

PLoS ONE, 2013
To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria needs to be precisely determined. The usual isolation methods such as differential centrifugation
Andras Franko, Olivier R Baris, Eva Bergschneider, Christine von Toerne, Stefanie M Hauck, Michaela Aichler, Axel K Walch, Wolfgang Wurst, Rudolf J Wiesner, Ian C D Johnston, Martin Hrabĕ de Angelis   +10 more
doaj   +1 more source

PURIFICATION OF TOMATO BUSHY STUNT VIRUS BY DIFFERENTIAL CENTRIFUGATION

Journal of Biological Chemistry, 1940
W. M. Stanley
openalex   +3 more sources

Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes

Memorias do Instituto Oswaldo Cruz, 2015
Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison.
S Andrea Moreno, Mayerly Nava
doaj   +1 more source

Investigation of Integral and Differential Characteristics of Variatropic Structure Heavy Concretes by Ultrasonic Methods

Applied Sciences, 2021
The article develops methods and methodology for experimental studies of centrifuged and vibro-centrifuged concrete products of annular cross-section. They assess the real variatropy of the structure and confirm the correctness of the accepted research ...
Levon R. Mailyan, Sergey A. Stel’makh, Evgenii M. Shcherban’, Alexander K. Khalyushev, Alla S. Smolyanichenko, Alexander K. Sysoev, Ivan A. Parinov, Alexander V. Cherpakov   +7 more
doaj   +1 more source

Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation

Acta Societatis Botanicorum Poloniae, 2012
A protocol is presented for low-cost and fast isolation of intact and pure chloroplasts from leaves of plants acclimated to low irradiance. The protocol is based on a differential centrifugation of cleared leaf homogenate and omits a centrifugation on ...
Magda Grabsztunowicz, Grzegorz Jackowski
doaj   +1 more source

Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation.

Analytical Chemistry, 2016
Extracellular vesicles (ECVs), including microparticles and exosomes, are submicrometer membrane vesicles released by diverse cell types upon activation or stress.
M. Rezeli, O. Gidlöf, M. Evander, Paulina Bryl-Górecka, R. Sathanoori, Patrik Gilje, K. Pawłowski, P. Horvatovich, D. Erlinge, G. Marko‐Varga, T. Laurell   +10 more
semanticscholar   +1 more source