Results 41 to 50 of about 2,730,068 (332)

Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification. [PDF]

open access: yesPLoS ONE, 2018
Isolated mitochondria are widely used to study the function of the organelle. Typically, mitochondria are prepared using differential centrifugation alone or in conjunction with density gradient ultracentrifugation.
Pin-Chao Liao   +4 more
doaj   +1 more source

Isolation of Microvesicles from Human Circulating Neutrophils

open access: yesBio-Protocol, 2021
Neutrophil-derived microvesicles (NDMVs) are liberated by neutrophils upon cell activation by molecules. Once activated, neutrophils are primarily involved in acute inflammation; however, the microvesicles they produce are largely anti-inflammatory ...
Dong Zhan   +5 more
doaj   +1 more source

Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage

open access: yesScientific Reports, 2022
The discovery and utility of clinically relevant circulating biomarkers depend on standardized methods that minimize preanalytical errors. Despite growing interest in studying extracellular vesicles (EVs) and cell-free messenger RNA (cf-mRNA) as ...
Hyun Ji Kim   +9 more
doaj   +1 more source

Distribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes in sucrose and percoll gradients [PDF]

open access: yes, 1984
Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes ...
Bowers   +31 more
core   +1 more source

Differential leukocyte count method for bovine low somatic cell count milk [PDF]

open access: yes, 2003
Whereas many differential leukocyte count methods for high somatic cell count (SCC) milk from mastitic cows are available, only a few have been developed for low SCC milk.
Burvenich, Christian   +4 more
core   +2 more sources

Modern methods of granulometric analysis of dusted materials containing nanoparticles [PDF]

open access: yesФизико-химические аспекты изучения кластеров, наноструктур и наноматериалов, 2018
In the work the differential and integral distribution of particles of dolomite flour and wings of zinc-containing welsh furnaces by size was studied by the method of analytical disk centrifugation with X-ray detection.
A.Yu. Ataeva   +3 more
doaj   +1 more source

Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes

open access: yesMemorias do Instituto Oswaldo Cruz, 2015
Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison.
S Andrea Moreno, Mayerly Nava
doaj   +1 more source

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