Results 41 to 50 of about 273,854 (260)

Differential Centrifugation Studies of Guinea Pig Lung Proteases

Experimental Biology and Medicine, 1978
SummaryFive subcellular fractions were isolated from guinea pig lung homogenates by differential centrifugation. These fractions were defined biochemically by the analysis of 18 enzymes representing different subcellular compartments. Succinate dehydrogenase and cytochrome oxidase distributed with the heavy mitochondrial fraction, while N-acetyl-β ...
L G, Ferren, W T, Stauber, G, Kalnitsky
openaire   +2 more sources

Update on controls for isolation and quantification methodology of extracellular vesicles derived from adipose tissue mesenchymal stem cells

Frontiers in Immunology, 2014
The research field on extracellular vesicles (EV) has rapidly expanded in recent years due to the therapeutic potential of EV. Adipose tissue human mesenchymal stem cells (ASC) may be a suitable source for therapeutic EV.A major limitation in the field ...
Marcella eFranquesa, Martin Johannes Hoogduijn, Elia eRipoll, Franka eLuk, Mahdi eSalih, Michiel G.H. Betjes, Juan eTorras, Carla eBaan, Josep Maria Grinyo, Ana Maria Merino   +9 more
doaj   +1 more source

Differential Neutralization of Spontaneous and Centrifuge-assisted Chlamydial Infectivity [PDF]

Journal of General Microbiology, 1979
Neutralization by specific antibody of a fast-killing variant strain of Chlamydia trachomatis, which showed high spontaneous infectivity for cell monolayers, was examined. It appeared that in spontaneous infection antibody-treated chlamydiae were neutralized by inhibition of attachment to cells.
S, Ainsworth, I, Allan, J H, Pearce
openaire   +2 more sources

Extraction of Polyphenols from Cydonia Oblonga Leaves and Their Effect on Mitochondrial Membrane Stability [PDF]

BIO Web of Conferences
Cydonia oblonga Mill. (quince) is a fruit tree native to Azerbaijan, Dagestan, Turkmenistan, and Iran, known by various names including Baher Dana in Urdu and Behi in Hindi.
Matmurotov Bakhtishod, Madrakhimova Sakhiba, Soliyev Nuriddin, Matchanov Alimjan   +3 more
doaj   +1 more source

Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes

Journal of Lipid Research, 1977
Homogenates of cultured hamster fibroblasts (BHK 21 cells) were fractionated by differential centrifugation into six main fractions: nuclear, mitochondrial, light mitochondrial, microsomal, soluble, and floating.
J Brotherus, O Renkonen
doaj   +1 more source

Isolation of Peroxisomes from Frozen Liver of Rat by Differential and Iodixanol Gradient Centrifugation

Separations, 2023
In the last decade, research has shown that most diseases are associated with organelle dysfunction in which metabolites play a crucial role or indicate specific processes.
Lina Baz, Mona Al-thepyani, Salha Algarni, Hana Gashlan   +3 more
doaj   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Comparison of Methods for Isolation and Characterization of Total and Astrocyte‐Enriched Extracellular Vesicles From Human Serum and Plasma

Journal of Extracellular Biology
Extracellular vesicles (EV) which play critical roles in intercellular communication, have garnered interest as biomarkers with researchers studying brain‐related disease processes due to their ability to be isolated from various biofluids. Astrocytes, a
Leandra K. Figueroa‐Hall, Kaiping Burrows, Ahlam M. Alarbi, Bethany N. Hannafon, Cole Hladik, Chibing Tan, Rajagopal Ramesh, Jennifer L. Stewart, Victoria B. Risbrough, Martin P. Paulus, T. Kent Teague   +10 more
doaj   +1 more source

Isolation and identification of exosomes from feline plasma, urine and adipose-derived mesenchymal stem cells

BMC Veterinary Research, 2021
Background Exosomes, internal proteins, lipids, and nucleic acids coated by phospholipid bilayer membranes, are one type of small extracellular vesicles, which can mediate cell-cell communication.
Dongsheng Li, Huina Luo, Huimin Ruan, Zhisheng Chen, Shengfeng Chen, Bingyun Wang, Yong Xie   +6 more
doaj   +1 more source