Results 61 to 70 of about 273,854 (260)

Methods for extracellular vesicles isolation in a hospital setting

Frontiers in Immunology, 2015
The research in extracellular vesicles (EVs) has been rising during the last decade. However, there is no clear consensus on the most accurate protocol to isolate and analyze them.
Matías eSáenz-Cuesta, Matías eSáenz-Cuesta, Ander eArbelaiz, Ander eArbelaiz, Amaia eOregi, Haritz eIrizar, Haritz eIrizar, Iñaki eOsorio-Querejeta, Iñaki eOsorio-Querejeta, Maider eMuñoz-Culla, Maider eMuñoz-Culla, Jesús M Bañales, Jesús M Bañales, Jesús M Bañales, Jesús M Bañales, Jesús M Bañales, Juan M Falcon-Perez, Juan M Falcon-Perez, Juan M Falcon-Perez, Javier eOlascoaga, Javier eOlascoaga, David eOtaegui, David eOtaegui   +22 more
doaj   +1 more source

Direct labeling of milk cells without centrifugation for counting total and differential somatic cells using flow cytometry [PDF]

, 2022
Jérôme Widmer, Laurence Descloux, Cédric Brügger, Marielle-Louise Jäger, Thomas F. H. Berger, Lotti Egger   +5 more
openalex   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Cytospin Centrifuge in Differential Counts of Milk Somatic Cells

Journal of Dairy Science, 1982
A procedure was developed for a cytospin centrifuge to concentrate cells from milk onto microscope slides in a circle with 6 mm diameter. Differential somatic cell counts with cytospin were compared to the conventional hand smearing technique to ascertain variation in differential counts.
A M, Dulin, M J, Paape, B T, Weinland
openaire   +2 more sources

Ion-exchange chromatography purification of extracellular vesicles

BioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović, Bojana Milutinović, Sanja Goč, Ninoslav Mitić, Miroslava Janković   +4 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Chloroplast DNA Extraction from Herbaceous and Woody Plants for Direct Restriction Fragment Length Polymorphism Analysis

BioTechniques, 2000
The technique described here is a fast and simple method of extracting chloroplast DNA (cpDNA). It overcomes the need for differential centrifugation using density gradients. The leaves do not have to be kept in the dark and lyophilized before extraction,
C. Mariac, P. Trouslot, C. Poteaux, G. Bezançon, J.-F. Renno   +4 more
doaj   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

O-209 Separating spermatozoa from debris, non-germ and red blood cells during differential centrifugation as a new effective approach of sperm recovery from surgically-retrieved specimens [PDF]

, 2022
E. E. Zakharova, V Zaletova
openalex   +1 more source