Results 71 to 80 of about 2,730,068 (332)

Isolation of Peroxisomes from Frozen Liver of Rat by Differential and Iodixanol Gradient Centrifugation

Separations, 2023
In the last decade, research has shown that most diseases are associated with organelle dysfunction in which metabolites play a crucial role or indicate specific processes.
Lina Baz, Mona Al-thepyani, Salha Algarni, Hana Gashlan   +3 more
doaj   +1 more source

Ultracentrifugation for ultrafine nanodiamond fractionation

, 2017
In this paper we propose a method for ultrafine fractionation of nanodiamonds using the differential centrifugation in the fields up to 215000g. The developed protocols yield 4-6 nm fraction giving main contribution to the light scattering intensity. The
Besedina, N. A., Eidelman, E. D., Kirilenko, D. A., Koniakhin, S. V., Shvidchenko, A. V.   +4 more
core   +1 more source

Three method-combination protocol for improving purity of extracellular vesicles [PDF]

, 2020
Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms.
Giamas, Georgios, Kumaran, Anish, Simon, Thomas, Veselu, Diana-Florentina   +3 more
core   +1 more source

The Hansenula polymorpha PER8 Gene Encodes a Novel Peroxisomal Integral Membrane Protein Involved in Proliferation [PDF]

, 1995
We previously described the isolation of mutants of the methylotrophic yeast Hansenula polymorpha that are defective in peroxisome biogenesis. Here, we describe the characterization of one of these mutants, per8, and the cloning of the PER8 gene.
Cregg, James M.,, Tan, Xuqiu,, Veenhuis, Marten,, Waterham, Hans R.,   +3 more
core   +3 more sources

The intracellular domain of TLR2 is capable of high‐affinity Zn binding: possible outcomes for the receptor activation

FEBS Letters, EarlyView.
Toll‐like receptors (TLRs) are important in the innate immune system. This study explores the zinc‐binding ability of the TLR2 TIR domain (TLR2TIR). We found that TLR2TIR binds zinc with nanomolar affinity through its cysteine residues. Two of them, C673 and C713, are vital for receptor activation, indicating that zinc may play a role in initiating ...
Vladislav A. Lushpa, Cong Lin, Irina A. Talyzina, Marina V. Goncharuk, Eduard V. Bocharov, Alexander S. Arseniev, Xiaohui Wang, Sergey A. Goncharuk, Konstantin S. Mineev   +8 more
wiley   +1 more source

Comparison of Methods for Isolation and Characterization of Total and Astrocyte‐Enriched Extracellular Vesicles From Human Serum and Plasma

Journal of Extracellular Biology
Extracellular vesicles (EV) which play critical roles in intercellular communication, have garnered interest as biomarkers with researchers studying brain‐related disease processes due to their ability to be isolated from various biofluids. Astrocytes, a
Leandra K. Figueroa‐Hall, Kaiping Burrows, Ahlam M. Alarbi, Bethany N. Hannafon, Cole Hladik, Chibing Tan, Rajagopal Ramesh, Jennifer L. Stewart, Victoria B. Risbrough, Martin P. Paulus, T. Kent Teague   +10 more
doaj   +1 more source

Update on controls for isolation and quantification methodology of extracellular vesicles derived from adipose tissue mesenchymal stem cells

Frontiers in Immunology, 2014
The research field on extracellular vesicles (EV) has rapidly expanded in recent years due to the therapeutic potential of EV. Adipose tissue human mesenchymal stem cells (ASC) may be a suitable source for therapeutic EV.A major limitation in the field ...
Marcella eFranquesa, Martin Johannes Hoogduijn, Elia eRipoll, Franka eLuk, Mahdi eSalih, Michiel G.H. Betjes, Juan eTorras, Carla eBaan, Josep Maria Grinyo, Ana Maria Merino   +9 more
doaj   +1 more source

Rapid isolation and purification of mitochondria for transplantation using tissue dissociation and differential filtration [PDF]

, 2016
Researchers have identified several methods for treating acute myocardial infarction (AMI) patients affected by ischemia and reperfusion injury. Some of these therapies include thrombolysis, balloon angioplasty, and coronary arterial bypass graft (CAGB).
Preble, Janine Marie
core   +1 more source

Transcriptomic and proteomic analyses of Desulfovibrio vulgaris biofilms: carbon and energy flow contribute to the distinct biofilm growth state. [PDF]

, 2012
BackgroundDesulfovibrio vulgaris Hildenborough is a sulfate-reducing bacterium (SRB) that is intensively studied in the context of metal corrosion and heavy-metal bioremediation, and SRB populations are commonly observed in pipe and subsurface ...
Arkin, Adam P, Clark, Melinda E, Fields, Matthew W, He, Zhili, Joachimiak, Marcin P, Keasling, Jay D, Mukhopadhyay, Aindrila, Redding, Alyssa M, Zhou, Jizhong Z   +8 more
core   +3 more sources

Refining the NaV1.7 pharmacophore of a class of venom‐derived peptide inhibitors via a combination of in silico screening and rational engineering

FEBS Letters, EarlyView.
Venom peptides have shown promise in treating pain. Our study uses computer screening to identify a peptide that targets a sodium channel (NaV1.7) linked to chronic pain. We produced the peptide in the laboratory and refined its design, advancing the search for innovative pain therapies.
Gagan Sharma, Jennifer R. Deuis, Xinying Jia, Theo Crawford, Sassan Rahnama, Eivind A. B. Undheim, Irina Vetter, Yanni K. ‐Y. Chin, Mehdi Mobli   +8 more
wiley   +1 more source