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Roadmap on computational methods in optical imaging and holography [invited]. [PDF]
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Quantification of mammalian tumor cell state plasticity with digital holographic cytometry
Quantitative Phase Imaging IV, 2018Individual cells within isogenic tumor populations can exhibit distinct cellular morphologies, behaviors, and molecular profiles. Cell state plasticity refers to the propensity of a cell to transition between these different morphologies and behaviors.
Jun Song +2 more
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Proceedings of SPIE, 2017
Digital holography is a useful technique for recording and reconstruction of the complex amplitude of an optical field. In this technique, an interference pattern of two waves is detected by an image sensor, and digital holograms are acquired in computer. The wavefront is reconstructed by a numerical calculation. In this study, we present the real-time
Yoshihisa Aizu, Hideki Funamizu
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Digital holography is a useful technique for recording and reconstruction of the complex amplitude of an optical field. In this technique, an interference pattern of two waves is detected by an image sensor, and digital holograms are acquired in computer. The wavefront is reconstructed by a numerical calculation. In this study, we present the real-time
Yoshihisa Aizu, Hideki Funamizu
exaly +2 more sources
Proceedings of SPIE, 2015
The in vitro cytotoxicity assessment of engineered nanoparticles commonly involves the measurement of different endpoints like the formation of reactive oxygen species, cell viability or cell death. Usually these parameters are determined by optical readouts of enzymatically converted substrates that often interfere with the tested nanomaterials. Using
Juergen Schnekenburger, Björn Kemper
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The in vitro cytotoxicity assessment of engineered nanoparticles commonly involves the measurement of different endpoints like the formation of reactive oxygen species, cell viability or cell death. Usually these parameters are determined by optical readouts of enzymatically converted substrates that often interfere with the tested nanomaterials. Using
Juergen Schnekenburger, Björn Kemper
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Journal of Biophotonics, 2019
AbstractWe combined Michelson‐interferometer‐based off‐axis digital holographic microscopy (DHM) with a common flow cytometry (FCM) arrangement. Utilizing object recognition procedures and holographic autofocusing during the numerical reconstruction of the acquired off‐axis holograms, sharply focused quantitative phase images of suspended cells in flow
Veselina Trendafilova +2 more
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AbstractWe combined Michelson‐interferometer‐based off‐axis digital holographic microscopy (DHM) with a common flow cytometry (FCM) arrangement. Utilizing object recognition procedures and holographic autofocusing during the numerical reconstruction of the acquired off‐axis holograms, sharply focused quantitative phase images of suspended cells in flow
Veselina Trendafilova +2 more
exaly +3 more sources
Quantitative imaging flow cytometry based on digital holographic microscopy
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 2023Natan T Shaked
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Advances in Microscopic Imaging V
Digital holographic microscopy is one of the effective techniques for quantitative phase imaging and plays an important role in bioimaging, such as measuring the thickness or refractive index of biological cells and cell morphology. In this study, we report the cell identification using machine learning of morphological parameters based on phase ...
Hideki Funamizu
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Digital holographic microscopy is one of the effective techniques for quantitative phase imaging and plays an important role in bioimaging, such as measuring the thickness or refractive index of biological cells and cell morphology. In this study, we report the cell identification using machine learning of morphological parameters based on phase ...
Hideki Funamizu
exaly +2 more sources
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 2023
Juergen Schnekenburger +2 more
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Juergen Schnekenburger +2 more
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Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 2023
Vicente Mico +2 more
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Vicente Mico +2 more
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