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Digital Holographic Microscopy to Assess Cell Behavior
2023Digital holographic microscopy is an imaging technique particularly well suited to the study of living cells in culture, as no labeling is required and computed phase maps produce high contrast, quantitative pixel information. A full experiment involves instrument calibration, cell culture quality checks, selection and setup of imaging chambers, a ...
Brad, Bazow +5 more
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Digital Holographic Microscopy (DHM)
Imaging & Microscopy, 2006Digital Holographic Microscopes (DHM) enables strictly noninvasive visualisation of unstained transparent, and partially reflective specimens, in real time, by providing simultaneously amplitude and phase changes of, a light wave transmitted or reflected.
Yves Emery +5 more
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Superesolution in digital holographic microscopy
2011 10th Euro-American Workshop on Information Optics, 2011In this contribution, we address with the possibility to overcome the limited resolving power of imaging systems beyond the limit imposed by Abbe's diffraction theory. We first review the mathematical foundations underlying superresolution (SR) from an information theory point of view and then we focus on two multiplexing approaches in digital ...
Alejandro Calabuig +4 more
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Digital Holography and Digital Holographic Microscopy
2015Whereas conventional analog holography (AH) entails physical re-illumination of the hologram of the object with a reading beam and finding the 3D real or virtual image, digital holography (DH) is a numerical procedure that simulates Fresnel diffraction of light from the re-illuminated hologram on a computer and numerically determines the images, which ...
Georges T. Nehmetallah +2 more
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Coherence effects in digital in-line holographic microscopy
Journal of the Optical Society of America A, 2008We analyze the effects of partial coherence in the image formation of a digital in-line holographic microscope (DIHM). The impulse response is described as a function of cross-spectral density of the light used in the space-frequency domain. Numerical simulation based on the applied model shows that a reduction in coherence of light leads to broadening
Unnikrishnan, Gopinathan +2 more
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Speckle-field digital polarization holographic microscopy
Optics Letters, 2019We present a new polarization holographic microscopy technique based on speckle-field illumination with enhanced spatial resolution and controlled coherent noise reduction. The proposed technique employs a spatial light modulator for the generation of a sequential speckle pattern for the illumination of the sample.
R. V. Vinu +4 more
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Dual plane in-line digital holographic microscopy
Optics Letters, 2010We report a dual plane in-line digital holographic microscopy technique that exploits the method of subtraction of average intensity of the entire hologram to suppress the zero-order diffracted wave. Two interferograms are recorded at different planes to eliminate the conjugate image.
Das, Bhargab, Yelleswarapu, Chandra S
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Digital holographic microscopy with physical phase compensation
Optics Letters, 2009An optical configuration for digital holographic microscopy is presented. Digital off-axis holograms are recorded by using a single-cube beam splitter in a nonconventional configuration so as to both split and combine a diverging spherical wavefront emerging from a microscope objective.
Qu, Weijuan +3 more
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Digital Holographic Microscopy
Imaging & Microscopy, 2009Björn Kemper +4 more
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