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Autofocus algorithms for digital-holographic microscopy

Biophotonics 2007: Optics in Life Science, 2007
Digital-holographic metrology enables quantitative phase contrast microscopy of reflective and (partially) transparent samples. In this way, new application fields are opened up for nondestructive investigations of technical samples as well as for marker-free and time-resolved analysis of cell biological processes.
Björn Kemper   +2 more
openaire   +2 more sources

Robust autofocus for digital-holographic microscopy

OSA Imaging and Applied Optics Congress 2021 (3D, COSI, DH, ISA, pcAOP), 2021
A new method to achieve autofocus in digital holography is proposed. It is based on the insertion of calibrated objects in the sample and the use of a robust algorithm to locate these objects.
Louis Thibon   +7 more
openaire   +2 more sources

Simultaneous Multiplane Imaging in Digital Holographic Microscopy

Journal of Display Technology, 2011
We describe a numerical method applied to the reconstruction of a digital hologram, that allows to obtain the image of an object at three planes at different depths simultaneously. In order to do this, we introduce a numerical quadratic deformed diffraction grating into the algorithm.
Paturzo Melania   +2 more
openaire   +6 more sources

Use of digital holographic microscopy in tomography

SPIE Proceedings, 2006
Digital Holographic Microscopy (DHM) provides three-dimensional (3D) images with a high vertical accuracy in the nanometer range and a diffracted limited transverse resolution. This paper focuses on 3 different tomographic applications based on DHM. First, we show that DHM can be combined with time gating: a series of holograms is acquired at different
Charrière, F.   +7 more
openaire   +3 more sources

Microlenses metrology with digital holographic microscopy

SPIE Proceedings, 2005
Digital holographic Microscopy (DHM) is an imaging modality reconstructing the wavefront in a numerical form, directly from a single digitalized hologram. It brings quantitative data derived simultaneously from the amplitude and phase of the complex reconstructed wavefront diffracted by the object and it is used to determine the refractive index and/or
Charrière, F.   +7 more
openaire   +3 more sources

Digital Holography and Digital Holographic Microscopy

2015
Whereas conventional analog holography (AH) entails physical re-illumination of the hologram of the object with a reading beam and finding the 3D real or virtual image, digital holography (DH) is a numerical procedure that simulates Fresnel diffraction of light from the re-illuminated hologram on a computer and numerically determines the images, which ...
Rola Aylo   +2 more
openaire   +2 more sources

Extended depth-of-focus by digital holographic microscopy

Optics Letters, 2010
A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of moving the sample.
Colomb T.   +5 more
openaire   +4 more sources

Digital holographic microscopy for silicon microsystems metrology

SPIE Proceedings, 2010
We propose to use digital holographic microscopy (DHM) with an illumination in the near infrared spectrum bandwidth, where the silicon is known to have small absorption. With such an illumination condition, it is possible to observe a wider range of specimens than in the visible spectrum, providing a new metrology technique for 3D silicon micro-systems
Delacretaz Y., Depeursinge C.
openaire   +3 more sources

Digital holographic microscopy applied to life sciences

2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2007
Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of erythrocyte cells. DHM has the advantage to be non-invasive (no phototoxicity, no contrast agents)
Depeursinge, C.   +6 more
openaire   +4 more sources

Recent Advances in Digital Holographic Microscopy

2018 20th International Conference on Transparent Optical Networks (ICTON), 2018
In digital holographic microscopy (DHM) a hologram is captured in the image space provided by a microscope. The transfer of the phase and amplitude structure in the original sample to the hologram is in fact strongly affected by the use of the imaging microscope.
Genaro Saavedra   +4 more
openaire   +2 more sources

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