Results 211 to 220 of about 32,871 (254)
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Autofocus algorithms for digital-holographic microscopy
Biophotonics 2007: Optics in Life Science, 2007Digital-holographic metrology enables quantitative phase contrast microscopy of reflective and (partially) transparent samples. In this way, new application fields are opened up for nondestructive investigations of technical samples as well as for marker-free and time-resolved analysis of cell biological processes.
Björn Kemper +2 more
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Simultaneous Multiplane Imaging in Digital Holographic Microscopy
Journal of Display Technology, 2011We describe a numerical method applied to the reconstruction of a digital hologram, that allows to obtain the image of an object at three planes at different depths simultaneously. In order to do this, we introduce a numerical quadratic deformed diffraction grating into the algorithm.
Paturzo Melania +2 more
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Digital Holography and Digital Holographic Microscopy
2015Whereas conventional analog holography (AH) entails physical re-illumination of the hologram of the object with a reading beam and finding the 3D real or virtual image, digital holography (DH) is a numerical procedure that simulates Fresnel diffraction of light from the re-illuminated hologram on a computer and numerically determines the images, which ...
Rola Aylo +2 more
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Extended depth-of-focus by digital holographic microscopy
Optics Letters, 2010A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of moving the sample.
Colomb T. +5 more
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Journal of The Optical Society of America A-optics Image Science and Vision, 2020
Digital holographic microscopy supplemented with the developed cell segmentation and machine learning and classification algorithms is implemented for quantitative description of the dynamics of cellular necrosis induced by photodynamic treatment in ...
A. Belashov +6 more
semanticscholar +1 more source
Digital holographic microscopy supplemented with the developed cell segmentation and machine learning and classification algorithms is implemented for quantitative description of the dynamics of cellular necrosis induced by photodynamic treatment in ...
A. Belashov +6 more
semanticscholar +1 more source
Digital Holographic Microscopy for the Life Sciences
Biomedical Optics and 3-D Imaging, 2012In the life sciences digital holographic microscopy provides high resolving, on-line, label-free, quantitative, multi-focus phase contrast imaging for the analysis of morphology dynamics and migration tracking in living cells.
Bjoern Kemper, Gert von Bally
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Realistic modeling of digital holographic microscopy
Optical Engineering, 2020A self-contained platform for realistic modeling of digital holographic microscopy (DHM) is presented. Amplitude and phase samples, imaged in different architectures, can be modeled to produce numerical DHM holograms that include all the parameters that are present in real experiments, providing an accessible way for newcomers to have a first approach ...
Jorge Garcia-Sucerquia +1 more
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Digital Holographic Microscopy in the Presence of Refraction
Imaging and Applied Optics 2016, 2016Refraction poses a problem in digital holographic microscopy for samples with a high numerical aperture (NA). We present raytracing and acquisition close to the image plane to enable refraction corrected phase imaging of such samples.
Jeroen Kalkman, Joseph van Rooij
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Digital holographic microscopy applied to metrology
SPIE Proceedings, 2004We report on a method called Digital Holographic Microscopy (DHM) for the numerical reconstruction of digital holograms taken with a microscope. It allows for simultaneous amplitude and quantitative phase contrast imaging. The reconstruction method computes the propagation of the complex optical wavefront diffracted by the object and is used to ...
Tristan Colomb +9 more
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Recent Advances in Digital Holographic Microscopy
2018 20th International Conference on Transparent Optical Networks (ICTON), 2018In digital holographic microscopy (DHM) a hologram is captured in the image space provided by a microscope. The transfer of the phase and amplitude structure in the original sample to the hologram is in fact strongly affected by the use of the imaging microscope.
Genaro Saavedra +4 more
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