Results 231 to 240 of about 48,162 (258)
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A fluorometric assay for dihydrofolate reductase
Analytical Biochemistry, 1977Abstract A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate ...
C. A. Lindquist +2 more
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Dihydrofolate reductases as targets for inhibitors
Advances in Enzyme Regulation, 1980Abstract Species differences among dihydrofolate reductases were first detected and most strikingly illustrated by the selective binding of small molecule inhibitors. This led on the one hand to practical applications in the chemotherapy of microbial infections, and on the other hand to stimuli for sequence and conformation studies of representative ...
Sheila L. Smith, George H. Hitchings
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Dihydrofolate Reductase Inhibitors [PDF]
, 1984 While exploring structural variations of anilinopyrimidines with antimalarial activity, a group at Imperial Chemical Industries found that certain biguanides synthesised as ring-opened analogues possessed good activity against Plasmodium gallinaceum infections in chicks (Curd et al. 1945).
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A histochemical method for dihydrofolate reductase
The Histochemical Journal, 1970A histochemical method for the detection of dihydrofolate reductase (5,6,7,8-tetrahydrofolate: NADP oxidoreductase, E.C. 1.5.1.3) is described. With this method, the highest enzyme activity has been found in hepatocytes, nephrocytes, intestinal epithelium and connective tissue cells.
E. Trutza +2 more
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Novel inhibitors of leishmanial dihydrofolate reductase [PDF]
Bioorganic & Medicinal Chemistry Letters, 2001 The program DOCK3.5 was used to search the Cambridge Structural Database for novel inhibitors of Leishmanial dihydrofolate reductase. A number of compounds were obtained and screened against the enzyme and against the intact parasite Leishmania donovani and the related organisms Trypanosoma brucei and Trypanosoma cruzi.
Chowdhury, Shafinaz F +7 more
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Structure of Dihydrofolate When Bound to Dihydrofolate Reductase
Journal of the American Chemical Society, 1998The Raman spectrum of dihydrofolate (H2folate) complexed with dihydrofolate reductase (DHFR) and NADP+, believed to be an accurate mimic of the productive DHFR/NADPH/H2folate complex involved in the reaction catalyzed by DHFR, contains bands associated with stretch motions of N5C6 of bound substrate.
Hua Deng, Robert Callender
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The kinetics of the histochemical method for dihydrofolate dehydrogenase (dihydrofolate reductase)
Histochemistry, 1979The purpose of the present investigation is to report some histochemical and cytospectrophotometric observations providng more objective evidence for the specific activity of the histochemical method for dihydrofolate reductase activity (5.6.7.8-tetrahydrofolate: E.C. 1.5.1.3).
Doina Onicescu, V. Atanasiu
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The interactions of dihydrofolate with M. tuberculosis dihydrofolate reductase
2016 Second Asian Conference on Defence Technology (ACDT), 2016The molecular dynamics (MD) simulation was used to determine the interactions and binding affinities of Mycobacterium tuberculosis dihydrofolate reductase (mtbDHFR) in complex with dihydrofolate (DHF), which is the natural substrate of the enzyme. The MD simulation approach can predict the ternary complexes of mtbDHFR with NADPH and DHF in the present ...
Luckhana Lawtrakul +2 more
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Inhibitors of Dihydrofolate Reductase
1983Methotrexate (1b), which remains the single most important antifol anticancer agent after 30 years of clinical use, owes its activity to its stoichiometric inhibition of the enzyme dihydrofolate reductase (EC 1. 5. 1. 3.). Methotrexate was introduced as an antileukaemic agent 10 years before dihydrofolate reductase was demonstrated to be its prime ...
B. Roth, C. R. Beddell, E. Bliss
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Properties of Brodimoprim as an Inhibitor of Dihydrofolate Reductases [PDF]
Chemotherapy, 1984 Based on both IC50 and Ki values, brodimoprim (BDP) was often found to be two- to threefold more effective than trimethoprim (TMP) against a great variety of different bacterial dihydrofolate reductases (DHFR). In all cases BDP was found to be a reversible, tight-binding inhibitor.
R.L. Then, F. Hermann
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