Structural studies on dihydrolipoyl transacetylase : the core component of the pyruvate dehydrogenase complex of Azotobacter vinelandii [PDF]
, 1988 The studies described in this thesis deal with the structure of the Azotobactervinelandii dihydrolipoyl transacetylase, the core component (E 2 ) of the pyruvate dehydrogenase complex. in all organisms the pyruvate dehydrogenase complex is closely related to the 2-oxoglutarate dehydrogenase complex and, if present, the branched-chain 2-oxoacid ...
J.R.O. Hanemaaijer
semanticscholar +6 more sources
Journal of Biological Chemistry, 1989 The dihydrolipoyl transacetylase (E2)-protein X-kinase subcomplex was resolved to produce an oligomeric transacetylase that was free of protein X and kinase subunits. We investigated the properties of this transacetylase E2 oligomer and of a form of the subcomplex from which only the lipoyl-bearing domain of protein X (XL) was removed.
SUSAN L. POWERS‐GREENWOOD +3 more
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Site‐directed mutagenesis of the dihydrolipoyl transacetylase component (E2p) of the pyruvate dehydrogenase complex from Azotobacter vinelandii [PDF]
European Journal of Biochemistry, 1991 Site‐directed mutagenesis was performed in the protease‐sensitive region, between the lipoyl and catalytic domains and in the catalytic domain, of the dihydrolipoyl transacetylase component (E2p) of the pyruvate dehydrogenase complex from Azotobacter vinelandii.
Egbert Schulze +3 more
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Time‐resolved fluorescence studies on mutants of the dihydrolipoyl transacetylase (E2) component of the pyruvate dehydrogenase complex from Azotobacter vinelandii [PDF]
FEBS Letters, 1990 Fluorescence anisotropy decays were measured for the wild‐type dihydrolipoyl transacetylase (E2) component of pyruvate dehydrogenase complex from Azotobacter vinelandii and E. coli and for E2‐mutants from A. vinelandii in which the alanine‐proline‐rich sequence between the binding domain and the catalytic domain is partially or completely deleted.
Egbert Schulze +3 more
semanticscholar +7 more sources
Sequential 1H and 15N nuclear magnetic resonance assignments and secondary structure of the N‐terminal lipoyl domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii [PDF]
European Journal of Biochemistry, 1994 The N‐terminal lipoyl domain (79 residues) of the transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii has been sub‐cloned and produced in Escherichia coli. Over‐expression exceeds the capacity of E. coli cells to lipoylate all expressed lipoyl domain, but addition of lipoic acid to the growth medium results in ...
Axel Berg, Arie de KOK, Jacques Vervoort
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The domain structure of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii [PDF]
European Journal of Biochemistry, 1987 Limited proteolysis with trypsin has been used to study the domain structure of the dihydrolipoyltransacetylase (E2) component of the pyruvate dehydrogenase complex of Azotobacter vinelandii. Two stable end products were obtained and identified as the N-terminal lipoyl domain and the C-terminal catalytic domain.
Roeland Hanemaaijer +3 more
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Subunit structure of dihydrolipoyl transacetylase component of pyruvate dehydrogenase complex from bovine heart. [PDF]
Journal of Biological Chemistry, 1981 D.M. Bleile +3 more
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Mechanism of formation and significance of antimitochondrial autoantibodies in the pathogenesis of primary biliary cholangitis [PDF]
Exploration of ImmunologyPrimary biliary cholangitis (PBC) is a chronic cholestatic progressive liver disease associated with cholangiopathies. The detection of antimitochondrial autoantibodies (AMAs) plays an important role in the diagnosis of classical PBC.
Vasiliy Ivanovich Reshetnyak +1 more
doaj +4 more sources
Mitochondria-Targeted Multifunctional Nanoparticles Combine Cuproptosis and Programmed Cell Death-1 Downregulation for Cancer Immunotherapy. [PDF]
Adv Sci (Weinh)Mitochondria‐targeted core–shell nanoparticle Cu/TI for simultaneous cuproptosis induction and PD‐L1 downregulation is reported. Cu/TI exhibits prolonged blood circulation and high tumor uptake to induce immunogenic cancer cell death and reduce PD‐L1 expression in vivo, thereby effectively enhancing the infiltration and activation of cytotoxic T ...
Li Y, Liu J, Weichselbaum RR, Lin W.
europepmc +5 more sources
A comparison of the large-scale gene expression patterns in summer and fall migratory Pantala flavescens (Fabricius) in northern China. [PDF]
Ecol EvolPantala flavescens (Fabricius) is the most well‐known seasonal migratory insect. A total of 624 differentially expressed genes (DEGs) with log2FC ≥ 2 were identified in summer migration compared to fall migration. Enrichment analysis showed that immunity, bacterial response and detoxification substances were significantly enriched in summer migration ...
Cao L, Wang N.
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