Results 11 to 20 of about 52 (52)

Time‐resolved fluorescence studies on mutants of the dihydrolipoyl transacetylase (E2) component of the pyruvate dehydrogenase complex from Azotobacter vinelandii [PDF]

FEBS Letters, 1990
Fluorescence anisotropy decays were measured for the wild‐type dihydrolipoyl transacetylase (E2) component of pyruvate dehydrogenase complex from Azotobacter vinelandii and E. coli and for E2‐mutants from A. vinelandii in which the alanine‐proline‐rich sequence between the binding domain and the catalytic domain is partially or completely deleted.
Schulze, E., Westphal, A.H., Berg, A., de Kok, A.   +3 more
openaire   +4 more sources

Recognition of the Inner Lipoyl-Bearing Domain of Dihydrolipoyl Transacetylase and of the Blood Glucose-Lowering Compound AZD7545 by Pyruvate Dehydrogenase Kinase 2 [PDF]

Biochemistry, 2007
Pyruvate dehydrogenase kinase 2 (PDHK2) is a unique mitochondrial protein kinase that regulates the activity of the pyruvate dehydrogenase multienzyme complex (PDC). PDHK2 is an integral component of PDC tightly bound to the inner lipoyl-bearing domains (L2) of the dihydrolipoyl transacetylase component (E2) of PDC.
Alina Tuganova, and Alla Klyuyeva, Kirill M. Popov   +2 more
openaire   +3 more sources

Catalysis of transthiolacylation in the active centers of dihydrolipoamide acyltransacetylase components of 2‐oxo acid dehydrogenase complexes

FEBS Open Bio, Volume 8, Issue 6, Page 880-896, June 2018., 2018
Two residues—Asp374 and His375—were identified to be catalytically important at the active center of the E2 component of the Escherichia coli 2‐oxoglutarate dehydrogenase complex. A series of experiments demonstrated that the mechanism of succinyl transfer between the thiol groups of dihydrolipoamide‐E2 and CoA does not involve acid–base catalysis ...
Joydeep Chakraborty, Natalia S. Nemeria, Edgardo Farinas, Frank Jordan   +3 more
wiley   +1 more source

Human Metabolic Enzymes Deficiency: A Genetic Mutation Based Approach

Scientifica, Volume 2016, Issue 1, 2016., 2016
One of the extreme challenges in biology is to ameliorate the understanding of the mechanisms which emphasize metabolic enzyme deficiency (MED) and how these pretend to have influence on human health. However, it has been manifested that MED could be either inherited as inborn error of metabolism (IEM) or acquired, which carries a high risk of ...
Swati Chaturvedi, Ashok K. Singh, Amit K. Keshari, Siddhartha Maity, Srimanta Sarkar, Sudipta Saha, Gary Lopaschuk   +6 more
wiley   +1 more source

Structure/function relationships in the pyruvate dehydrogenase complex form Azotobacter vinelandii [PDF]

European Journal of Biochemistry, 1993
The role of the hinge region between the binding domain and the catalytic domain in dihydrolipoyl transacetylase (E2p) from Azotobacter vinelandii was addressed by deletion mutagenesis. Mutated dihydrolipoyl transacetylase proteins were constructed with a deletion of 11 amino acids in the hinge region between the binding domain and the N‐terminal part ...
Schulze, E., Westphal, A.H., Hanemaaijer, R., de Kok, A.   +3 more
openaire   +4 more sources

Domain Structures of the Dihydrolipoyl Transacetylase and the Protein X Components of Mammalian Pyruvate Dehydrogenase Complex

Journal of Biological Chemistry, 1989
Treatment of the dihydrolipoyl transacetylase-protein X-kinase subcomplex (E2-X-KcKb) with protease Arg C selectively converted protein X into an inner domain fragment (Mr approximately equal to 35,000) and an outer (lipoyl-bearing) domain fragment (Mr approximately equal to 15,500).
Gary A. Radke, S. Gopalakrishnan, Mohammed Rahmatullah, Thomas E. Roche   +3 more
openaire   +3 more sources

Separation of Protein X from the Dihydrolipoyl Transacetylase Component of the Mammalian Pyruvate Dehydrogenase Complex and Function of Protein X

Journal of Biological Chemistry, 1989
The dihydrolipoyl transacetylase (E2)-protein X-kinase subcomplex was resolved to produce an oligomeric transacetylase that was free of protein X and kinase subunits. We investigated the properties of this transacetylase E2 oligomer and of a form of the subcomplex from which only the lipoyl-bearing domain of protein X (XL) was removed.
Susan L. Powers‐Greenwood, Gary A. Radke, Mohammed Rahmatullah, Thomas E. Roche   +3 more
openaire   +3 more sources

Copper homeostasis and cuproptosis‐related genes: Therapeutic perspectives in non‐alcoholic fatty liver disease

Diabetes, Obesity and Metabolism, Volume 26, Issue 11, Page 4830-4845, November 2024.
Abstract Non‐alcoholic fatty liver disease (NAFLD), a metabolic‐associated fatty liver disease, has become the most common chronic liver disease worldwide. Recently, the discovery of cuproptosis, a newly identified mode of cell death, further highlighted the importance of copper in maintaining metabolic homeostasis. An increasing number of studies have
Wangjing Tan, Junli Zhang, Li Chen, Yayuan Wang, Rui Chen, Haiming Zhang, Fengxia Liang   +6 more
wiley   +1 more source

Mitochondria‐Targeted Multifunctional Nanoparticles Combine Cuproptosis and Programmed Cell Death‐1 Downregulation for Cancer Immunotherapy

Advanced Science, Volume 11, Issue 35, September 18, 2024.
Mitochondria‐targeted core–shell nanoparticle Cu/TI for simultaneous cuproptosis induction and PD‐L1 downregulation is reported. Cu/TI exhibits prolonged blood circulation and high tumor uptake to induce immunogenic cancer cell death and reduce PD‐L1 expression in vivo, thereby effectively enhancing the infiltration and activation of cytotoxic T ...
Youyou Li, Jing Liu, Ralph R. Weichselbaum, Wenbin Lin   +3 more
wiley   +1 more source

A comparison of the large‐scale gene expression patterns in summer and fall migratory Pantala flavescens (Fabricius) in northern China

Ecology and Evolution, Volume 14, Issue 8, August 2024.
Pantala flavescens (Fabricius) is the most well‐known seasonal migratory insect. A total of 624 differentially expressed genes (DEGs) with log2FC ≥ 2 were identified in summer migration compared to fall migration. Enrichment analysis showed that immunity, bacterial response and detoxification substances were significantly enriched in summer migration ...
Lingzhen Cao, Na Wang
wiley   +1 more source