Results 41 to 50 of about 5,638 (129)

Establishment of an experimental system to analyse extracellular vesicles during apoplastic fungal pathogenesis

Journal of Extracellular Biology, Volume 4, Issue 2, February 2025.
Abstract Phoma stem canker disease of oilseed rape (Brassica napus) is caused by the extracellular fungal pathogen Leptosphaeria maculans. Although this pathogen resides exclusively in apoplastic spaces surrounding plant cells, the significance of extracellular vesicles (EVs) has not been assessed.
Nathaniel Hearfield, Dominik Brotherton, Zedi Gao, Jameel Inal, Henrik U. Stotz   +4 more
wiley   +1 more source

Identification of peptidases in Nicotiana tabacum leaf intercellular fluid. [PDF]

, 2008
Peptidases in the extracellular space might affect the integrity of recombinant proteins expressed in, and secreted from, plant cells. To identify extracellular peptidases, we recovered the leaf intercellular fluid from Nicotiana tabacum plants by an ...
Boutry, Marc, Vertommen, Didier, Georges Alves, Ma, Julian, Julian Ma, Alves, Georges, Mélanie Delannoy, Didier Vertommen, Marc Boutry, Delannoy, Mélanie, Navarre, Catherine, Catherine Navarre   +11 more
core   +1 more source

In Silico Evaluation of Human α‐S1‐Casein, β‐Casein, and κ‐Casein as a Source of Bioactive Peptides

Journal of Food Biochemistry, Volume 2025, Issue 1, 2025.
Human caseins (α‐S1, β, and κ‐casein) represent a potential source of bioactive peptides with possible therapeutic applications, particularly for neonates. Due to the relevance of obtaining bioactive peptides, an in silico simulation of enzymatic hydrolysis with pepsin (pH > 2) was performed on these proteins using the PeptideCutter tool from Expasy ...
Diego Arturo Zavala Trejo, Blanca Rosa Aguilar Uscanga, Ariana Rodríguez Arreola, Esperanza González Quezada, Josué Raymundo Solís Pacheco, Anupama Bose   +5 more
wiley   +1 more source

Current advances and therapeutic potential of agents targeting dipeptidyl peptidases-IV, -II, 8/9 and fibroblast activation protein [PDF]

, 2011
[[abstract]]Dipeptidyl peptidase-IV (DPP-IV), a serine protease that specifically cleaves the N-terminal dipeptide with a preference for L-proline or L-alanine at the penultimate position, is involved in the degradation of incretin hormones, including ...
Chen, SJ;Jiaang, WT
core   +1 more source

Preparation, Identification, and Cellular Antioxidant Activity Evaluation of Antioxidant Peptides From the Antler Tips of Sika Deer (Cervus nippon)

Journal of Food Biochemistry, Volume 2025, Issue 1, 2025.
A process for extracting the antler tip peptides (ATPs) from Sika deer (Cervus nippon) antler tips was optimized using a method that combines single‐factor experiment and response surface methodology. The optimal conditions involved papain as the hydrolase, an enzyme concentration of 5000 U/g, a hydrolysis time of 3.0 h, a temperature of 52.0°C, a pH ...
Xingyu Xiao, Jilun Meng, Xi Chen, Yi Li, Shuo Zheng, Xunming Zhang, Libo Zhang, Jiayuan Fang, Tong Su, Ze Ma, Hong Zhang, Linlin Hao, Yogesh Kumar   +12 more
wiley   +1 more source

Radiation inactivation analysis of kidney microvillar peptidases [PDF]

, 1986
Five membrane peptidases were studied by radiation inactivation analysis of pig kidney microvillar membranes. One heterodimeric enzyme, γ-glutamyl transferase, presented a target size corresponding to the dimeric Mr.
Ian S. Fulcher, Jean Ingram, A.John Kenny, Kenny, A.John, Fulcher, Ian S., Ingram, Jean   +5 more
core   +1 more source

Optimization of ultracentrifugation‐based method to enhance the purity and proteomic profiling depth of plasma‐derived extracellular vesicles and particles

Journal of Extracellular Biology, Volume 3, Issue 7, July 2024.
Abstract Circulating extracellular vesicles and particles (EVPs) are being investigated as potential biomarkers for early cancer detection, prognosis, and disease monitoring. However, the suboptimal purity of EVPs isolated from peripheral blood plasma has posed a challenge of in‐depth analysis of the EVP proteome. Here, we compared the effectiveness of
Zurong Wan, Jinghua Gu, Uthra Balaji, Linda Bojmar, Henrik Molina, Søren Heissel, Alexandra E. Pagano, Christopher Peralta, Lee Shaashua, Dorina Ismailgeci, Hope K. Narozniak, Yi Song, William R. Jarnagin, David P. Kelsen, Jaqueline Bromberg, Virginia Pascual, Haiying Zhang   +16 more
wiley   +1 more source

Chemoproteomics-enabled identification of 4-Oxo-beta-Lactams as inhibitors of dipeptidyl peptidases 8 and 9 [PDF]

, 2022
Dipeptidyl peptidases 8 and 9 (DPP8/9) have gathered interest as drug targets due to their important roles in biological processes like immunity and tumorigenesis.
Rita Félix, Podlesainski, D., Tiago Rodrigues, Ross, B., Moreira, R., Wöhrle, Svenja, Rodrigues, Tiago, Lucas, S., Carvalho, L., Lum, K., Kaiser, Markus, Vieira, Andreia C., Geiss-Friedlander, R., Woehrle, S., Breyan Ross, Ross, Breyan, Rui Moreira, Bolgi, Oguz, Kiefersauer, Reiner, Cravatt, Benjamin F., Rodrigues, T., Lorenz Fehr, Podlesainski, David, Kaiser, M., Huber, R., Reiner Kiefersauer, David Podlesainski, Groß, Olaf, Andreia C. Vieira, Lucas, Susana D., Markus Kaiser, Oguz Bolgi, Luís A. R. Carvalho, Moreira, Rui, Fehr, L., Carvalho, Luis A. R., Robert Huber, Geiss-Friedlander, Ruth, Cravatt, B., Vieira, A., Susana D. Lucas, Olaf Groß, Félix, Rita, Svenja Wöhrle, Huber, Robert, Fehr, Lorenz, Felix, R., Kenneth M. Lum, Gross, O., Benjamin F. Cravatt, Bolgi, O., Ruth Geiss‐Friedlander, Kiefersauer, R., Lum, Kenneth M.   +53 more
core   +1 more source

Kallikrein-related peptidases. [PDF]

, 2008
Kallikrein 1 (KLK1), a key component of the kallikrein-kinin system, originates from a locus on the long arm of chromosome 19 that contains several related serine endopeptidases.
Lundwall, Åke, Brattsand, M
core   +3 more sources

Exploring the root canal microbiome in previously treated teeth: A comparative study of diversity and metabolic pathways across two geographical locations

International Endodontic Journal, Volume 57, Issue 7, Page 885-894, July 2024.
Abstract Aim To analyse and compare the root canal microbiome present in root‐filled teeth of two different geographical populations, and to study their functional potential using a next‐generation sequencing approach. Methodology Sequencing data obtained from surgical specimens from previously treated teeth with periapical bone loss from Spain and USA
M. T. Arias‐Moliz, R. Ordinola‐Zapata, C. Staley, V. Pérez‐Carrasco, J. A. García‐Salcedo, D. Uroz‐Torres, M. Soriano   +6 more
wiley   +1 more source