Results 1 to 10 of about 620,062 (268)

Accuracy of the direct agglutination test for diagnosis of visceral leishmaniasis: a systematic review and meta-analysis [PDF]

BMC Infectious Diseases, 2023
Background Parasitological investigation of bone marrow, splenic or lymph node aspirations is the gold standard for the diagnosis of visceral leishmaniasis (VL).
Tamalee Roberts, Suzanne H. Keddie, Sayaphet Rattanavong, Santiago Rayment Gomez, John Bradley, Ruth H. Keogh, Oliver Bärenbold, Jane Falconer, Petra F. Mens, Heidi Hopkins, Elizabeth A. Ashley   +10 more
doaj   +5 more sources

Validation of rK39 immunochromatographic test and direct agglutination test for the diagnosis of Mediterranean visceral leishmaniasis in Spain. [PDF]

PLoS Neglected Tropical Diseases, 2018
Visceral leishmaniasis (VL), the most severe form of leishmaniasis, is endemic in Europe with Mediterranean countries reporting endemic status alongside a worrying northward spread. Serological diagnosis, including immunochromatographic test based on the
Mathieu Bangert, María D Flores-Chávez, Ivonne P Llanes-Acevedo, Carolina Arcones, Carmen Chicharro, Emilia García, Sheila Ortega, Javier Nieto, Israel Cruz   +8 more
doaj   +8 more sources

Good agreement of conventional and gel-based direct agglutination test in immune-mediated haemolytic anaemia [PDF]

Acta Veterinaria Scandinavica, 2012
Background The aim of this study was to compare a gel-based test with the traditional direct agglutination test (DAT) for the diagnosis of immune-mediated haemolytic anaemia (IMHA).
Piek Christine J, Teske Erik, van Leeuwen Martin W, Day Michael J   +3 more
doaj   +5 more sources

The diagnostic accuracy of direct agglutination test for serodiagnosis of human visceral leishmaniasis: a systematic review with meta-analysis. [PDF]

BMC Infect Dis, 2020
Background Direct agglutination test (DAT) as a simple, accurate and reliable method, has been widely used for serodiagnosis of visceral leishmaniasis (VL) during the last three decades.
Mohebali M, Keshavarz H, Shirmohammad S, Akhoundi B, Borjian A, Hassanpour G, Mamishi S, Mahmoudi S.   +7 more
europepmc   +6 more sources

Experimental Evidence on the Nature of the Antigen in the Direct Agglutination Test for Visceral Leishmaniasis. [PDF]

Am J Trop Med Hyg, 2020
The direct agglutination test (DAT) for visceral leishmaniasis (VL) is the serodiagnostic test for VL that has the most robust sensitivity and specificity in the field across all endemic regions. It is based on trypsin-treated and formaldehyde-fixed whole promastigote cells from Leishmania donovani.
Kühne V, Verstraete R, van Ostade X, Büscher P.   +3 more
europepmc   +6 more sources

A comparative study of nested-PCR and direct agglutination test (DAT) for the detection of Leishmania infantum infection in symptomatic and asymptomatic domestic dogs. [PDF]

BMC Res Notes, 2021
Canine visceral leishmaniasis (CVL) is the main source of human visceral leishmaniosis (HVL) in Mediterranean region, including Iran and is spread from domestic dogs to Phlebotomine sand flies vectors to humans.
Yimam Ayene Y, Mohebali M, Hajjaran H, Akhoundi B, Shojaee S, Rahimi-Foroushani A, Afshar MJA, Zarei Z.   +7 more
europepmc   +4 more sources

Diagnostic accuracy of direct agglutination test, rK39 ELISA and six rapid diagnostic tests among visceral leishmaniasis patients with and without HIV coinfection in Ethiopia. [PDF]

PLoS Negl Trop Dis, 2020
Diagnosis of a first-time visceral leishmaniasis (VL) infection in Ethiopia is established by use of a rapid diagnostic test (RDT) detecting antibodies against rK39, direct agglutination test (DAT) and microscopy according to the national algorithm.
Kassa M, Abdellati S, Cnops L, Bremer Hinckel BC, Yeshanew A, Hailemichael W, Vogt F, Adriaensen W, Mertens P, Diro E, van Griensven J, Van den Bossche D.   +11 more
europepmc   +4 more sources

Modifications in a Reference Freeze-Dried Direct Agglutination Test to Improve Visceral Leishmaniasis Detection. [PDF]

Am J Trop Med Hyg, 2020
Currently, a significantly lower temperature (35°C) than initially established (56°C) is indicated as the maximum temperature storage for the commercial reference visceral leishmaniasis (VL) freeze-dried direct agglutination test (FD-DAT).
Harith AE, Awad Y, Mahamoud A, Abass E, Mansour D, Moura de Melo C, Madi RR, Semiao-Santos SJ, Osman HA.   +8 more
europepmc   +4 more sources

The Unknown Nature of the Antigen in the Direct Agglutination Test for Visceral Leishmaniasis Hampers Development of Serodiagnostic Tests. [PDF]

Am J Trop Med Hyg, 2019
Current diagnostic tests for visceral leishmaniasis (VL) are either not adapted for use in resource-poor settings or are insufficiently accurate in Eastern Africa. Only the direct agglutination test (DAT), based on whole Leishmania promastigotes, is highly reliable in all endemic regions, but its implementation is hampered by the need for a cold chain,
Kühne V, Büscher P.
europepmc   +6 more sources

Validation of in-house liquid direct agglutination test antigen: the potential diagnostic test in visceral Leishimaniasis endemic areas of Northwest Ethiopia [PDF]

BMC Microbiology, 2020
Background Visceral leishmaniasis in Ethiopia is a re-emerging threat to public health, with increased geographical distribution and number of cases.
Birhanu Ayelign, Mohammedamin Jemal, Markos Negash, Meaza Genetu, Tadelo Wondmagegn, Ayalew Jejaw Zeleke, Ligabaw Worku, Abebe Genetu Bayih, Girma Shumie, Sinknesh Wolde Behaksra, Tiruwork Fenta, Demekech Damte, Arega Yeshanew, Endalamaw Gadisa   +13 more
doaj   +3 more sources