An evaluation of the performance of direct agglutination test on filter paper blood sample for the diagnosis of visceral leishmaniasis. [PDF]
The attack phase of the visceral leishmaniasis (VL) elimination program in Bangladesh aims to decrease the burden of VL incidence from close to 20 cases to less than one case per 10,000 at sub-district level.
Hasnain MG, Ghosh P, Baker J, Mondal D.
europepmc +2 more sources
Multi‐centre evaluation of repeatability and reproducibility of the direct agglutination test for visceral leishmaniasis [PDF]
Summary objective To evaluate the repeatability and reproducibility of the serological direct agglutination test (DAT) for visceral leishmaniasis (VL) with aqueous antigen in a multi‐centre study in VL‐endemic areas in Sudan, Kenya and Nepal. methods Repeatability within each centre and reproducibility between the centres' results and an external ...
Marleen Boelaert+10 more
openalex +6 more sources
Improvement of a direct agglutination test for field studies of visceral leishmaniasis [PDF]
To increase the potential for the wide-scale application of our direct agglutination test for visceral leishmaniasis, modifications in the components and procedures were introduced. Supplementation with 0.056 M citrate of the suspension medium stabilized the antigen for 9 weeks at 37 degrees C. To circumvent the need for cooling systems in the field, 0.
Tinka Jelsma+6 more
openaire +4 more sources
DIRECT AGGLUTINATION TEST WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS
A new direct agglutination test (DAT) for use with urine samples for the diagnosis of visceral leishmaniasis (VL) has been developed and compared with the conventional DAT with serum samples and our previously reported enzyme-linked immunosorbent assay (ELISA) with urine samples (urine ELISA).
Mohammad Zahidul Islam+8 more
openalex +3 more sources
Objective: To compare diagnostic accuracy and agreement between direct agglutination test and rK39 rapid tests for diagnosis of visceral leishmaniasis in an endemic area, the Doka area in Eastern Sudan.
Elfadil Abass
doaj +2 more sources
Humoral immune response in Oreochromis niloticus: modified direct agglutination test [PDF]
Understanding the functional mechanisms of the humoral and cellular immune responses among teleost broadens the possibilities for intervention and improvement of vaccination methods. The aim of this study was to evaluate the dynamics of the humoral immune response, hematological biochemical changes, and electrophoretic profile of proteins in ...
Fernandes, Dayanne C.+10 more
openaire +5 more sources
Modified Direct Agglutination Test for Simplified Serologic Diagnosis of Leishmaniasis
ABSTRACT Human leishmaniasis is a severe health problem in many countries around the world. Hence, a cheap, reliable, and accurate diagnostic test is required to fight this disease. Perhaps the direct agglutination test (DAT) meets these criteria, but antigen elaboration involves many difficulties. We have developed a new antigen
Pablo Gómez-Ochoa+7 more
openalex +5 more sources
Assessment of Recombinant A2-Latex Agglutination Test (RA2-LAT) and RA2-ELISA for Detection of Canine Visceral Leishmaniasis: A Comparative Field Study with Direct Agglutination Test in Northwestern Iran [PDF]
Background: This study aimed to set-up latex agglutination test (LAT) and ELISA based on recombinant A2 from Iranian strain of Leishmania (L.) infantum (rA2-Ag) and evaluated for detection of anti- Leishmania antibodies in dogs compared to standard ...
Mahin FARAHMAND+7 more
doaj +1 more source
Evaluation of Two rK39 Dipstick Tests, Direct Agglutination Test, and Indirect Fluorescent Antibody Test for Diagnosis of Visceral Leishmaniasis in a New Epidemic Site in Highland Ethiopia [PDF]
We assessed the performance characteristics of two rK39 immunochromatographic tests, a direct agglutination test (DAT), and an indirect immunofluorescent antibody test (IFAT) in the site of a new epidemic of visceral leishmaniasis (VL) in northwestern ...
Carmen Cañavate+10 more
openalex +2 more sources
Rapid detection of Leishmania infantum infection in dogs: a comparative study using fast agglutination screening test (FAST) and direct agglutination test (DAT) in Iran [PDF]
Canine visceral leishmaniasis (CVL) is not only a veterinary problem but has also a serious public health importance. Rapid detection of CVL is highly important for control of human visceral leishmaniasis in Iran. This study was aimed to compare the fast agglutination screening test (FAST) with direct agglutination test (DAT) as a standard serological ...
Leila Babakhan+4 more
openalex +4 more sources