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Limitations of Direct Immunofluorescence-Reply
Archives of Dermatology, 1992In Reply.— We appreciate Bystryn's comments and agree that a more appropriate site selection for immunofluorescence biopsy in patients in whom systemic lupus erythematosus is being considered is a less exposed area. Our study, however, was meant to address the rate of "false" positivity on an exposed surface such as the head or neck.
Jeffrey P. Callen +2 more
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Direct and indirect immunofluorescent findings in dermatomyositis
Journal of Cutaneous Pathology, 1985Three skin biopsies (proximal nailfold, extensor forearm and buttock) and serum samples were studied by direct and indirect immunofluorescence, immunodiffusion and radioimmunoassay techniques in 6 patients with dermatomyositis. A variety of seroligic and immunopathologic abnormalities was observed in the patients.
Richard M. Silver +5 more
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Limitations of Direct Immunofluorescence
Archives of Dermatology, 1992To the Editor.— The article by Fabre et al 1 on the presence of granular deposits of immunoglobulin at the basement membrane zone of normal sun-exposed skin is valuable in pointing out some of the limitations of direct immunofluorescence for the diagnosis of lupus erythematosus.
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Direct immunofluorescence in the diagnosis of scleroderma syndromes
British Journal of Dermatology, 1977Immunofluorescent study of the skin of nine patients with mesenchymal, inflammatory scleroderma (mixed connective tissue disease) revealed immunoglobulin and complement deposition at the basement membrane or within blood vessel walls. The skin specimens of ten patients with systemic scleroderma were negative for immunofluorescence.
Francisco J. Carapeto +2 more
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International Journal of Dermatology, 2009
AbstractBackground Direct immunofluorescence (DIF) of perilesional skin is the gold standard in the diagnosis of pemphigus. Since the outer root sheath (ORS) of anagen hair is structurally analogous to epidermal keratinocytes, pemphigus specific immunofluorescence pattern may be present in the ORS.Methods Twenty consecutive patients of pemphigus were
Raghavendra Rao +3 more
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AbstractBackground Direct immunofluorescence (DIF) of perilesional skin is the gold standard in the diagnosis of pemphigus. Since the outer root sheath (ORS) of anagen hair is structurally analogous to epidermal keratinocytes, pemphigus specific immunofluorescence pattern may be present in the ORS.Methods Twenty consecutive patients of pemphigus were
Raghavendra Rao +3 more
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Direct Immunofluorescent Labeling of Cells
2009In the direct immunofluorescent labeling technique, fluorochrome-labeled antibodies are used as probes for particular antigens or biomolecules. Cells, usually after appropriate fixation, are incubated with the antibodies to which fluorochromes have been directly conjugated.
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Direct Immunofluorescence Diagnosis of Pemphigus without Biopsy
Journal of Cutaneous Medicine and Surgery, 1998Background: Direct immunofluorescence (DIF) is a necessary examination tool for the diagnosis of pemphigus. The suction-blister-method splits the skin at the lamina lucida and it is possible with a scalpel to separate the entire epidermis from the dermis.
Feliciani, C. +5 more
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Epidermolysis Bullosa Acquisita With Negative Direct Immunofluorescence
Archives of Dermatology, 1985• Epidermolysis bullosa (EB) appeared in a patient at the age of 54 years. Other bullous disorders could be excluded by electron microscopy, and there was no family history of EB. The patient would therefore best be classified as having EB acquisita.
Jean Paul Ortonne, Jean-Philippe Lacour
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Distinct Pattern of Direct Immunofluorescence in Livedoid Vasculopathy
The American Journal of Dermatopathology, 2010There are discrepancies in findings on direct immunofluorescence (DIF) examinations for livedoid vasculopathy. We sought to assess the usefulness of DIF examinations as an adjunct to the diagnosis of livedoid vasculopathy. Clinical data and findings on DIF examinations were retrospectively collected from our immunofluorescence laboratory database on 27
Pa-Fan Hsiao, Yu-Hung Wu
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Cicatricial pemphigoid: Direct and indirect immunofluorescent studies
Oral Surgery, Oral Medicine, Oral Pathology, 1981Oral mucosa, skin tissue, and serum samples from thirty-three patients with cicatricial pemphigoid were studied by the direct and indirect immunofluorescent techniques to determine the presence of tissue-bound and circulating antibodies. A linear continuous basement membrane zone pattern was observed in 96.9 percent of the oral mucosa biopsy specimens ...
A. Angelopoulos, George Laskaris
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