Results 41 to 50 of about 83,954 (263)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Shear strength parameters of jointed rock mass based on single test sample method

Yantu gongcheng xuebao, 2020
The difference between the samples affects the discreteness of the direct shear test results and more importantly the rationality of the analysis results of the shear strength parameters of the multiple test sample method of jointed rock mass.
DENG Hua-feng , XIONG Yu , XIAO Yao , QI Yu , LI Tao , XU Xiao-liang
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Shear Characteristics of Soil—Concrete Structure Interaction Interfaces

Applied Sciences, 2022
The shear characteristics of the interfaces between soil and concrete structures are essential for the safety of the structures. In this study, a large-scale direct shear test apparatus was developed to measure the mechanical parameters of soil–concrete ...
Dejie Li, Chong Shi, Huaining Ruan, Bingyi Li   +3 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Theoretical solution for the relationship between strength parameters of direct shear test and triaxial test

Yantu gongcheng xuebao
This paper analyzes the variation law of the Mohr's stress circle for normally consolidated soils in direct shear tests and triaxial compression tests. By comparing and analyzing the stress paths during the shearing process of the two tests, it is found ...
CAI Jifeng 1, CAI Jian 1, HU Zhongxiong 2
doaj   +1 more source

Artificial Intelligence in Systemic Sclerosis: Clinical Applications, Challenges, and Future Directions

Arthritis Care &Research, EarlyView.
Systemic sclerosis (SSc) is a rare autoimmune disease defined by immune dysregulation, vasculopathy, and progressive fibrosis of the skin and internal organs. Despite advances in care, major complications such as interstitial lung disease (ILD) and myocardial involvement remain the leading causes of morbidity and mortality.
Cristiana Sieiro Santos, José Miguel Sequí‐Sabater, Diego Benavent   +2 more
wiley   +1 more source

Large-Scale Direct Shear Test on Tire Slice Reinforced Crushed Concrete Particles

Advances in Materials Science and Engineering, 2020
In order to study the mechanical properties of tire slices reinforced crushed concrete particles, a series of shear tests were carried out under the conditions of different vertical loads, different tire volume contents, and different shear rates.
Qiang Ma, Hang Shu, Jia Mou, Lihua Li, Zhenyi Zheng   +4 more
doaj   +1 more source

Experimental Evaluation of 100Cr6 Steel Microindented Surfaces Under Lubricated Nonconformal Point Contacts

Advanced Engineering Materials, EarlyView.
The tribological behavior of 100Cr6 steel spheres textured via Vickers microindentation is evaluated under lubricated sliding by varying both dimple size and density. Fine and dense textures significantly reduce friction across all lubrication regimes, while large dimples increase it.
Farideh Davoodi, Pasquale Guglielmi, Gianfranco Palumbo, Giuseppe Carbone   +3 more
wiley   +1 more source

Direct shear characteristics of cement stabilized clay subjected to previous shear [PDF]

E3S Web of Conferences
The cement stabilization techniques have been applied to various types of infrastructure and become one of the essential techniques. A lot of researches have been conducted on the physical and mechanical properties of the cement stabilized soils, many of
Kitazume Masaki, Kasama Kiyonobu, Ueda Tomorou   +2 more
doaj   +1 more source