Results 181 to 190 of about 269,742 (193)

Directed Evolution of a Genetically Encoded Indicator for Chloride. [PDF]

ACS Synth Biol
Peng W, Tutol JN, Phelps SM, Kam H, Lynd JK, Dodani SC.   +5 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Modular determinants of cleavage preference in GIY-YIG nucleases revealed by block-based DNA shuffling and directed evolution. [PDF]

Nucleic Acids Res
Loedige KW, Hall MA, Ghimirey S, Stead BE, Edgell DR.   +4 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Directed Evolution of T7 RNA Polymerase Minimizes dsRNA By-product and Enables High-Fidelity mRNA Synthesis for Demanding Therapeutic Applications. [PDF]

Research (Wash D C)
Qin W, Nie T, Hei M, Li L, Pan Y, Luo M, Yang GY.   +6 more
europepmc   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Directed evolution of LaccID for cell surface proximity labeling and electron microscopy. [PDF]

Nat Chem Biol
Lee SY, Roh H, Gonzalez-Perez D, Mackey MR, Hoces D, McLaughlin CN, Lin C, Adams SR, Nguyen K, Kim KY, Luginbuhl DJ, Luo L, Udeshi ND, Carr SA, Hernández-López RA, Ellisman MH, Alcalde M, Ting AY.   +17 more
europepmc   +1 more source

A Golden Gate compatible system for continuous directed evolution in <i>E. coli</i>. [PDF]

Synth Biol (Oxf)
Sparrow Muñoz I, Burgess SJ.
europepmc   +1 more source

Directed Evolution of AtMP2 Peptide: Unlocking Enhanced Antibacterial Potential from <i>Anabas testudineus</i>. [PDF]

Molecules
Lee LT, Ang A, Najm A, Adnan AM, Nordin AM, Mahmood I, Dunkhorol S, Fazry S, Law D.   +8 more
europepmc   +1 more source

Directed evolution of phages in biofilms enhances Pseudomonas aeruginosa control through improved lipopolysaccharide recognition. [PDF]

Nat Commun
Meneses L, Valentová L, Santos SB, Erdmann J, Crabbé A, Plevka P, Häussler S, Pires DP, Coenye T, Azeredo J.   +9 more
europepmc   +1 more source