Results 191 to 200 of about 11,830,288 (332)

Directed Evolution of Plant-Associated Bacteria Enhances Plant Holobiont Stress Tolerance. [PDF]

Plant Cell Environ
Ravanbakhsh M, Xiong W.
europepmc   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Directed evolution of an enantiocomplementary S_NArase reveals divergent catalytic features. [PDF]

Nat Commun
Lister TM, Roberts GW, Duran C, Casadevall G, Zhao F, Millman AAV, Larrosa I, Osuna S, Green AP.   +8 more
europepmc   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

FEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana, Xiaowei Bogetti, Stuti Sharma, Ivet Bahar   +3 more
wiley   +1 more source

Directed evolution of the bacteriophage endolysin PlyC reveals a charge-mediated mechanism of thermal stabilization. [PDF]

J Biol Chem
Heselpoth RD, Nelson DC.
europepmc   +1 more source

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

FEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés, Luise Franz, Christian P.R. Hackenberger   +2 more
wiley   +1 more source

Correction: Directed evolution of a stem-helix-targeting antibody enables MERS-CoV cross-neutralization through enhanced binding affinity. [PDF]

PLoS Pathog
PLOS Pathogens Staff.
europepmc   +1 more source

Comparing the paths of Hedgehog release in artificial and natural settings

FEBS Letters, EarlyView.
Hh morphogens require solubilization factors to overcome their tight association with the plasma membrane and signal to distant cells, but the precise functions of these factors are controversial. Here, we use published in vivo observations as the most relevant evidence to test their suggested functions and discuss contradictions and gaps between in ...
Janna Puschmann, Kay Grobe
wiley   +1 more source

Directed evolution of compact RNA-guided nucleases for enhanced activity in mammalian cells. [PDF]

Genome Biol
Gorbenko F, Sala I, Lee YY, van de Venn L, Yeh CD, Tálas A, Karvelis T, Druteika G, Bechter LV, Vykhlyantseva I, Schröder MS, Gvozdenovic A, Schwank G, Siksnys V, Corn JE.   +14 more
europepmc   +1 more source