Results 71 to 80 of about 24,168 (302)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Linguistic Content of a Discourse Competence of Technical University Students [PDF]
, 2019 The article is devoted to the problem of discourse competence formation of technical university students. The substantiation of the linguistic content of a discourse competence is given, which includes knowledge of the genre-stylistic features of the ...
Хоменко, Е. В.
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FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
The Discourse of Management and the Management of Discourse [PDF]
Discourse is a pervasive tool of management; one might even say that discourse is what managers do. A widespread assumption among managers is that discourse is not only a pervasive tool, but an effective one for precise communication of information, for ...
Mahoney, Joseph T. +3 more
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Tłumaczenie jako świadczenie usług tłumaczeniowych?
Między Oryginałem a Przekładem, 2013 Translation as provision of translation services? At the crossroads between enhancing professional realism and serving the needs of the industry in translator education The paper seeks to analyze the current discourse in the field of translator ...
Joanna Dybiec‑Gajer
doaj +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Digital competence on the political agenda : A discourse analysis [PDF]
, 2018 The purpose of this Master’s thesis is to study why the concept of digital competence previously has been frequently addressed in Swedish politics, and to examine possible connections to the related concepts information literacy and media and information
Karlsson, Johanna
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FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
The Effect of Reading Interest and Achievement Motivation on Students’ Discourse Analysis Competence [PDF]
, 2020 Discourse analysis involves analyzing the language being used. This research investigates the effect of reading interest and achievement motivation on students’ discourse analysis competence.
Aprilia, Fitria +2 more
core +2 more sources
Registered Nurses’ Patient Education in Everyday Primary Care Practice
Global Qualitative Nursing Research, 2015 Nurses’ patient education is important for building patients’ knowledge, understanding, and preparedness for self-management. The aim of this study was to explore the conditions for nurses’ patient education work by focusing on managers’ discourses about
Anne-Louise Bergh +3 more
doaj +1 more source