Results 91 to 100 of about 603,154 (305)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test

Journal of Veterinary Research
Dimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in in vitro tests for detection of anthelmintic resistance of gastrointestinal nematodes (GIN).
Mickiewicz Marcin, Nowek Zofia, Czopowicz Michał, Moroz-Fik Agata, Potărniche Adrian-Valentin, Biernacka Kinga, Szaluś-Jordanow Olga, Górski Paweł, Antonopoulos Alistair, Markowska-Daniel Iwona, Várady Marián, Kaba Jarosław   +11 more
doaj   +1 more source

Viability of Methicillin-Resistant Staphylococcus aureus (MRSA) Bacteria Preserved at Different Temperature and Time

Quagga
The bacteria Staphylococcus aureus, a normal component of human flora, is easily mutated and antibiotic-resistant, leading to the creation of Methicillin-Resistant Staphylococcus aureus (MRSA).
Sekar Karima Rachma, Kurniawan, Ikhsan Mujahid, Kurnia Ritma Dhanti   +3 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Enhanced Pair Hydrophobicity in the Water-Dimethylsulfoxide (DMSO) Binary Mixture at Low DMSO Concentrations

, 2010
We observe a surprisingly sharp increase in the pair hydrophobicity in the water climethylsulfoxide (DMSO) binary mixture at small DMSO concentrations, with the mole fraction of DMSO (x(D)) in the range 0.12-0.16.
Saikat Banerjee, Biman Bagchi, Bagchi, Biman, Roy, Susmita, Banerjee, Saikat, Susmita Roy   +5 more
core   +1 more source

Effect of dimethylsulfoxide on sphingomyelinase activity and cholesterol metabolism in Niemann-Pick type C fibroblasts

Brazilian Journal of Medical and Biological Research, 1999
Niemann-Pick type C (NPC) fibroblasts present a large concentration of cholesterol in their cytoplasm due to a still unidentified deficiency in cholesterol metabolism.
F.B. Scalco, R. Giugliani, P. Tobo, J.C. Coelho   +3 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

EPR and electrochemistry of [NH4]trans-[RuCl4(DMSO)(L)] complexes (L = DMSO, py ). X-ray molecular structure of [pyH][RuCl4(DMSO)(py)]

, 2000
The [(DMSO)2H]trans-[RuCl4 (DMSO)2] complex presents an axial symmetry in the solid state and its EPR spectrum shows two g values (g^ or = 2,35 e g// or = 1,87). The complex [NH4]trans-[RuCl4(DMSO) 2] presents only one-g value in the solid state EPR
Bonfadini Marcos R., Costa-Filho Antônio J. da, Paula Quêite A. de, Schultz Mário S., Oliva Glaucius, Batista Alzir A., Nascimento Otaciro R.   +6 more
core  

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source