Results 51 to 60 of about 33,443 (169)

Genome wide DNA methylation analysis of Haloferax volcanii H26 and identification of DNA methyltransferase related PD-(D/E)XK nuclease family protein HVO_A0006

Frontiers in Microbiology, 2015
Restriction-modification (RM) systems have evolved to protect the cell from invading DNAs and are composed of two enzymes: a DNA methyltransferase and a restriction endonuclease. Although RM systems are present in both archaeal and bacterial genomes, DNA
Matthew eOuellette, Laura eJackson, Scott eChimileski, R. Thane ePapke   +3 more
doaj   +1 more source

Seasonal Difference of Gut Microbiota at a Stopover Site in Five Passerines of Different Migratory Behavior

Integrative Zoology, EarlyView.
Seasonal variation significantly reshaped gut microbiota composition in migratory passerine species, whereas the resident species Suthora webbiana showed no clear seasonal shift. Species with similar migratory behaviors exhibited consistent seasonal patterns in their core gut microbiota.
Jundong Duan, Amina Tufail, Xiaoping Wang, Tingbei Bo, Na Zhao, Yifan Yue, Sandi Willows‐Munro, Yanhua Qu, Jianshi Jin, Fumin Lei, Chenxi Jia, Gang Song   +11 more
wiley   +1 more source

Recent Insights Into the Structure, Function, and Evolution of the RNA-Splicing Endonucleases

Frontiers in Genetics, 2019
RNA-splicing endonuclease (EndA) cleaves out introns from archaeal and eukaryotic precursor (pre)-tRNA and is essential for tRNA maturation. In archaeal EndA, the molecular mechanisms underlying complex assembly, substrate recognition, and catalysis have
Akira Hirata
doaj   +1 more source

Testing for Bacteria and Fungi in Cells and Ancillary Reagents—Probe‐Based Quantitative PCR Assays

Cell Proliferation, EarlyView.
This document specifies the technical elements for bacteria and fungi detection by PCR assay which enables rapid, broad‐spectrum detection of bacteria and fungi with high sensitivity. Qualitative judgement is based on LOD. Key detection points include efficient nucleic acid extraction, broad‐spectrum primer‐probe design, high amplification efficiency ...
Jiani Cao, Ranran Xu, Boqiang Fu, Jie Hao, Lei Wang, Aijin Ma, Qiang Wei, Hongling Zhao, Chunming Rao, Qiyuan Li, Tao Na, Shijun Hu, Lihua Zhou, Weiqi Zhang, Qubo Chen, Siming Sun, Zengshu Liu, Shuaishuai Niu, Tingting Zhang, Yuanyuan Zhang, Yidi Gan, Shuyan Wang, Glyn N. Stacey, Jun Wei, Tongbiao Zhao, Yu Alex Zhang   +25 more
wiley   +1 more source

S‐Adenosylmethionine (SAM) hydrolases counter increased SAM epimerisation in thermophilic archaea

The FEBS Journal, EarlyView.
S‐Adenosyl‐l‐methionine (SAM) is a vital enzyme cofactor. Epimerisation at the sulfonium centre of biologically active (SS,SCα)‐SAM is driven by heat, yielding biologically inactive (RS,SCα)‐SAM. Here, two novel archaeal SAM hydrolases from the thermophilic Sulfolobus acidocaldarius and the halophilic Haloferax volcanii are shown to cleave (RS,SCα)‐SAM.
Agnes Bartels, Michael K. F. Mohr, Phillip Nußbaum, Marie Joest, Bianca Wassmer, Laurent Rasquin, Sonja‐Verena Albers, Jennifer N. Andexer   +7 more
wiley   +1 more source

A distinct gene expression mechanism for d‐amino acid utilization in hyperthermophilic archaeon Pyrococcus horikoshii

The FEBS Journal, EarlyView.
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami, Tomoki Kawase, Taira Uehara, Haruhiko Sakuraba, Tomohiko Matsuzawa, Junji Hayashi, Toshihisa Ohshima   +6 more
wiley   +1 more source

Ori-Finder 2, an integrated tool to predict replication origins in the archaeal genomes

Frontiers in Microbiology, 2014
DNA replication is one of the most basic processes in all three domains of cellular life. With the advent of the post-genomic era, the increasing number of complete archaeal genomes has created an opportunity for exploration of the molecular mechanisms ...
Hao eLuo, Chun-Ting eZhang, Feng eGao
doaj   +1 more source

Crystal structure of human NIF3‐like protein reveals dynamic hexameric assembly with a single divalent metal binding site

The FEBS Journal, EarlyView.
We present the first crystal structures of human NIF3, revealing a dynamic, hexameric ring with a central cavity regulated by flexible insertion domains. Crucially, human NIF3 features a mononuclear zinc‐binding site, marking a distinct evolutionary departure from the dinuclear centers of bacterial homologs.
Elżbieta Wątor‐Wilk, Krzysztof Żak, Piotr Wilk, Paweł Kochanowski, Agnieszka Maślanka, Łukasz Skalniak, Przemysław Grudnik   +6 more
wiley   +1 more source

The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool

Microbiome, 2018
Background Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion.
Jo De Vrieze, Ameet J. Pinto, William T. Sloan, Umer Zeeshan Ijaz   +3 more
doaj   +1 more source

Shotgun Metagenomic Analysis Reveals Taxonomic and Functional Transitions in the Salivary Microbiome During Periodontal Disease Progression

Journal of Clinical Periodontology, EarlyView.
ABSTRACT Aim To characterise multi‐kingdom salivary microbiome profiles across clinically defined periodontal states and identify stage‐specific taxonomic and functional alterations using shotgun metagenomic sequencing. Materials and Methods In this cross‐sectional study, 204 adults (mean age 40.3 ± 7.6 years) from the SECRETO study (NCT01934725 ...
Muhammed Manzoor, Jaakko Leskelä, Eija Könönen, Leo Lahti, Jukka Putaala, Pirkko J. Pussinen, Susanna Paju   +6 more
wiley   +1 more source