Results 41 to 50 of about 33,443 (169)

Processing of matched and mismatched rNMPs in DNA by archaeal ribonucleotide excision repair

iScience, 2023
Summary: Ribonucleoside monophosphates (rNMPs) are the main non-canonical nucleotides in genomic DNA, and their incorporation can occur as mismatches or matches in vivo.
Maurane Reveil, Lucie Chapel, Blandine Vourc’h, Audrey Bossé, Léa Vialle, Raphaël Brizard, Yann Moalic, Mohamed Jebbar, Ghislaine Henneke   +8 more
doaj   +1 more source

Beyond Chemical Compliance: A Critical Review Toward Microbiome‐Informed Ecological Exit Criteria for Bioremediated Soils

Land Degradation &Development, EarlyView.
ABSTRACT Chemical compliance remains the usual endpoint for closing contaminated‐soil remediation projects, although it does not establish recovery of soil function or the durability of the post‐treatment state. This critical review examines when microbiological evidence can contribute to ecological closure decisions.
Mariusz Cycoń
wiley   +1 more source

Evolution of replicative DNA polymerases in archaea and their contributions to the eukaryotic replication machinery

Frontiers in Microbiology, 2014
The elaborate eukaryotic DNA replication machinery evolved from the archaeal ancestors that themselves show considerable complexity. Here we discuss the comparative genomic and phylogenetic analysis of the core replication enzymes, the DNA polymerases ...
Kira S Makarova, Mart eKrupovic, Eugene V. Koonin   +2 more
doaj   +1 more source

Metagenome datasets from women with polycystic ovary syndrome from Irkutsk, Eastern Siberia, Russia

Data in Brief, 2020
For the metagenomic characterization of potential taxonomic and functional diversity of microorganisms associated with polycystic ovary syndrome (PCOS) in women, we surveyed five women with PCOS and collected samples of feces, saliva, and serum.
Natalia Belkova, Elizaveta Klimenko, Naliia Vilson, Alexsandra Sambialova, Daria Markova, Ilia Igumnov, Larisa Suturina   +6 more
doaj   +1 more source

Cotton strips as a model substrate for analysis of leaf‐litter‐colonizing microbial assemblages in streams

Limnology and Oceanography: Methods, EarlyView.
Abstract Leaf‐litter decomposition is a key indicator of the functional integrity of stream ecosystems and cotton strips have been employed as a standard substrate for quantification of litter‐decomposition rates in streams across the globe. Cotton strips also offer a standardized method for assessing structural integrity of stream microbial ...
Trinity Shoemaker, Cynthia Nguyen, Scott D. Tiegs, David M. Costello, John J. Kelly   +4 more
wiley   +1 more source

Remote Homology Detection Identifies a Eukaryotic RPA DBD-C-like DNA Binding Domain as a Conserved Feature of Archaeal Rpa1-Like Proteins

Frontiers in Molecular Biosciences, 2021
The eukaryotic single-stranded DNA binding factor replication protein A (RPA) is essential for DNA replication, repair and recombination. RPA is a heterotrimer containing six related OB folds and a winged helix-turn-helix (wH) domain.
Stuart A. MacNeill
doaj   +1 more source

An (αβ)2 Architecture of the Arabidopsis thaliana tRNA Splicing Endonuclease Complex: Structural Modeling and Evolutionary Analyses

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The tRNA splicing endonuclease (TSEN) removes introns from precursor tRNAs (pre‐tRNAs) in eukaryotes and archaea, but the architecture and subunit organization of plant TSEN remain poorly defined. Here, we integrated in planta bimolecular fluorescence complementation (BiFC) assays with AlphaFold3 (AF3)‐based structural modeling, molecular ...
Mohammad Moniruzzaman, Markus Englert, Kazuhito Akama   +2 more
wiley   +1 more source

The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes

Biology Direct, 2012
Background In eukaryotes, the CMG (CDC45, MCM, GINS) complex containing the replicative helicase MCM is a key player in DNA replication. Archaeal homologs of the eukaryotic MCM and GINS proteins have been identified but until recently no homolog of the ...
Makarova Kira S, Koonin Eugene V, Kelman Zvi   +2 more
doaj   +1 more source

Seasonal Dynamics of Community and Function of Gut Microbiome in Taihangshan Macaque (Macaca mulatta tcheliensis): Inferred From Metagenomic Data

Integrative Zoology, EarlyView.
A total of 435 non‐redundant metagenome‐assembled genomes (MAGs) were obtained from fecal samples of Taihangshan macaque across all four seasons. The alpha diversity of the gut microbiome in spring and winter was significantly higher than in summer and autumn. Furthermore, marked differences were detected in functional pathways and carbohydrate enzymes
Yanyan Zhou, Qi Shao, Chunbo Liu, Jundong Tian, Xiaowen Guan, Xi Zhang, Jiqi Lu   +6 more
wiley   +1 more source

Archaeal histone-based chromatin structures regulate transcription elongation rates

Communications Biology
Many archaea encode and express histone proteins to compact their genomes. Archaeal and eukaryotic histones share a near-identical fold that permits DNA wrapping through select histone-DNA contacts to generate chromatin-structures that must be traversed ...
Breanna R. Wenck, Robert L. Vickerman, Brett W. Burkhart, Thomas J. Santangelo   +3 more
doaj   +1 more source