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Accelerated Genomic Evolution and Divergence of <i>Escherichia coli</i> Under Phage Infection Stress: Emphasizing the Role of IS Elements in Changing Genetic Structure. [PDF]
Wen S, Yuan L, Li Y, Yin J, Luo P.
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Moss-Accumulated eDNA Is a Promising Source for Terrestrial Biodiversity Surveys Across the Tree of Life and Biomes. [PDF]
Lankes HFN +10 more
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Evaluation of the antimicrobial effect of cannabidiol (CBD) in a multispecies subgingival biofilm model. [PDF]
Tolentino PHMP +9 more
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Bringing the ends together: cryo-EM structures of mycobacterial Ku in complex with DNA define its role in NHEJ synapsis. [PDF]
Baral J +7 more
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Preparing Bacterial Genomic DNA
Cold Spring Harbor Protocols, 2022We describe two alternative procedures for purifying bacterial chromosomal DNA. The first procedure incorporates the use of a commercial kit based on silica membrane technology. This approach relies on the selective binding of DNA to a silica-based column in the presence of chaotropic salts (guanidine salts).
Nara Figueroa-Bossi +2 more
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Chloramphenicol damages bacterial DNA
Biochemical and Biophysical Research Communications, 1977L(+)-threo-chloramphenicol induces reversion of His−Salmonella typhimurium strains TA100 and TA1535 in the conventional Ames' assay without microsomal activation. Any mutagenicity of D(−)-threo-chloramphenicol was masked by toxicity. Similarly, a sensitive fluctuation test showed mutagenesis with L(+)-threo-chloramphenicol at concentrations of 0.5 μM ...
S F, Jackson +3 more
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Bacterial DNA excision repair pathways
Nature Reviews Microbiology, 2022Bacteria are continuously exposed to numerous endogenous and exogenous DNA-damaging agents. To maintain genome integrity and ensure cell survival, bacteria have evolved several DNA repair pathways to correct different types of DNA damage and non-canonical bases, including strand breaks, nucleotide modifications, cross-links, mismatches and ...
Katherine J. Wozniak, Lyle A. Simmons
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Comparing Bacterial DNA Microarray Fingerprints
Statistical Applications in Genetics and Molecular Biology, 2005Epidemiologic and forensic investigations often require assays to detect subtle genetic differences between closely related microorganisms. Typically, gel electrophoresis is used to compare randomly amplified DNA fragments between microbial samples, where the patterns of DNA fragment sizes are viewed as genotype fingerprints.
Willse, Alan +5 more
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