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Separation of Choanoflagellate and Bacterial Genomic DNA
Cold Spring Harbor Protocols, 2009INTRODUCTIONChoanoflagellates are heterotrophic nanoflagellates: small, colorless protozoa that are present in marine and freshwater environments as well as in hydrated soils. Because they are the closest living relatives of the metazoa, the study of their cell biology and genomes promises to provide new insights into metazoan ancestry and origins ...
Nicole, King +4 more
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Preservation of Bacterial DNA by Human Dentin
Journal of Endodontics, 2014The capacity of dentin and collagen to bind DNA and protect against spontaneous and nuclease-induced degradation was evaluated individually and by the incubation of DNA with nuclease-producing bacteria in a mixed culture.Extracted Fusobacterium nucleatum DNA was incubated with dentin shavings or collagen for 90 minutes.
Malin, Brundin +3 more
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DNA transfer and DNA synthesis during bacterial conjugation
Zeitschrift f�r Vererbungslehre, 1966Mutant strains ofEscherichia coli, which were thermosensitive with respect to DNA replication, were used for conjugation experiments at 37°C and 42°C. Inhibition of DNA synthesis in the donor strain has no influence on the yield of recombinats. Inhibition of DNA synthesis in the recipient strain is accompanied by a complete loss of recombinant ...
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Bacterial topoisomerases and the control of DNA supercoiling
Trends in Genetics, 1990DNA in bacterial cells is under negative superhelical tension, a feature that facilitates many of the activities of DNA. Supercoiling is introduced enzymatically by DNA gyrase, and the accumulation of excessively high levels is prevented by the relaxing activity of DNA topoisomerase I.
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The Antigenicity of Bacterial DNA
2002DNA is a complex macromolecule whose immunological properties vary with base sequence and backbone structure. Although now recognized as important for normal immunity, the antigenic and immunogenic properties of DNA were originally conceptualized entirely in the context of systemic lupus erythematosus (SLE).
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Hybridization of Bacterial DNA on Filters
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Beyond horizontal gene transfer: the role of plasmids in bacterial evolution
Nature Reviews Microbiology, 2021Jeronimo Rodríguez-Beltrán +2 more
exaly
DNA Supercoiling Catalyzed by Bacterial Gyrase
DNA gyrase is an essential type II topoisomerase that is conserved across bacteria species and has an essential function of resolving overwound (positive supercoiled) DNA and introducing negative supercoils into relaxed DNA. The overall catalytic activity of gyrase can be determined using in vitro assays utilizing purified enzyme subunits and a DNA ...Samika, Joshi, Neil, Osheroff
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