Results 91 to 100 of about 428,487 (304)

Photosensitivity and heat resistance conferred by BrdU incorporation upon a thymidine kinase-deficient mouse cell line with persistent mitochondrial enzyme activity [PDF]

, 1975
The selective incorporation of 5-bromodeoxyuridine (BrdU) into mitochondrial DNA (mit-DNA) in the LM(TK-) ClID cell line, a thymidine kinase-deficient derivative of L fibroblasts with persistent mitochondrial enzyme activity, has been utilized to ...
Attardi, G., Keeley, Benneta, Tu, Catherina   +2 more
core  

Plasma extrachromosomal circular DNA as a biomarker in EGFR‐targeted therapy of non‐small cell lung cancer

Molecular Oncology, EarlyView.
Detection of extrachromosomal circular DNA (eccDNA) in plasma samples from EGFR‐mutated non‐small cell lung cancer patients. Plasma was collected before and during treatment with the EGFR‐tyrosine kinase inhibitor osimertinib. Plasma eccDNA was detected in all cancer samples, and the presence of the EGFR gene on eccDNA serves as a potential biomarker ...
Simone Stensgaard, Sarmad Mehmood, Eva Boysen Fynboe Ebert, Peter Meldgaard, Anindya Dutta, Boe S. Sorensen   +5 more
wiley   +1 more source

Spontaneous assembly of an organic-inorganic nucleic acid Z-DNA double-helix structure [PDF]

, 2017
Herein, we report a hybrid polyoxometalate organic–inorganic compound, Na2[(HGMP)2Mo5O15]⋅7 H2O (1; where GMP=guanosine monophosphate), which spontaneously assembles into a structure with dimensions that are strikingly similar to those of the naturally ...
Albrecht, Arnott, Barnes, Braga, Cafferty, Cairns-Smith, Carraro, Champ, Cooper, Crick, Ellena, Hill, Humphrey, Inoue, Kim, Kulikov, Kwak, Li, Maehigashi, Martins, Murayama, Nordén, Parker, Powner, Rich, Ritson, Soghomonian, Strandberg, Sun, Tang, Van Hecke, Vasconcelos, Voet, Wang, Wong, Yamase, Yu   +36 more
core   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

SPECTROSCOPIC AND PHYSICOCHEMICAL METHODS FOR STUDYING THE INTERACTION OF METALLOPORPHYRIN WITH DNA

Indonesian Journal of Chemistry, 2010
In recent years studies on the interaction of porphyrin with DNA have received much attention because of the importance in DNA-probing and photodynamic therapy of cancer. A variety of spectroscopic methods, e.g. NMR, ESR, Mössbauer, UV-visible absorption,
Hidenari Inoue
doaj   +1 more source

Topological entropy of a stiff ring polymer and its connection to DNA knots

, 2001
We discuss the entropy of a circular polymer under a topological constraint. We call it the {\it topological entropy} of the polymer, in short. A ring polymer does not change its topology (knot type) under any thermal fluctuations.
Brian A. A., Chen Y. D., de Gennes P. G., Dean F. B., Deguchi T., Deguchi T., Deguchi T., Deguchi T., Delbück M., des Cloizeaux J., Frisch H. L., Grosberg A., Grosberg A. Yu., Janse van Rensburg E. J., Klenin K. V., Koniaris K., Le Bret M., Madras N., Michels J. P. J., Odijk T., Orlandini E., Polyak M., Rybenkov V. V., Shaw S. Y., Shishido K., Stiger D., Vologodskii A. V., Walba D. M.   +27 more
core   +1 more source

Identification of G-quadruplex DNA/RNA binders: Structure-based virtual screening and biophysical characterization [PDF]

, 2017
Background Recent findings demonstrated that, in mammalian cells, telomere DNA (Tel) is transcribed into telomeric repeat-containing RNA (TERRA), which is involved in fundamental biological processes, thus representing a promising anticancer target. For
Alcaro, Stefano, Artese, Anna, Costa, Giosu\ue8, Distinto, Simona, Maccioni, Elias, Moraca, Federica, Nadai, Matteo, Ortuso, Francesco, Richter, Sara, Rocca, Roberta, Scalabrin, Matteo, Talarico, Carmine   +11 more
core   +1 more source

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

DNA Ministrings: Highly Safe and Effective Gene Delivery Vectors

Molecular Therapy: Nucleic Acids, 2014
Conventional plasmid DNA vectors play a significant role in gene therapy, but they also have considerable limitations: they can elicit adverse immune responses because of bacterial sequences they contain for maintenance and amplification in prokaryotes ...
Nafiseh Nafissi, Samih Alqawlaq, Eric A Lee, Marianna Foldvari, Paul A Spagnuolo, Roderick A Slavcev   +5 more
doaj   +1 more source

DNA Confined in Nanochannels: Hairpin Tightening by Entropic Depletion

, 2006
A theory is presented of DNA hairpins enclosed in a nanochannel. A hairpin becomes constrained as it approaches the wall of a channel which leads to an entropic force causing the hairpin to tighten.
Odijk, Theo
core   +2 more sources