Results 141 to 150 of about 2,856,737 (303)

Comparative Evaluation of DNA Extraction Protocols for Filamentous Fungi Using Cost-Effective and Broadly Accessible Methods

open access: yesAfrican and Mediterranean Agricultural Journal - Al Awamia
Reliable extraction of high-quality genomic DNA is essential for accurate molecular identification, population genetics, and plant disease diagnostics in filamentous fungi. However, DNA recovery is often hampered by the complex and resistant structure of
Imane Hourmatallah   +5 more
doaj   +1 more source

The power of many: when genetics met yeasts and high‐throughput

open access: yesBiological Reviews, EarlyView.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

DNA Barcoding of Fungi in the Forest Ecosystem of the Psunj and Papuk Mountains in Croatia

open access: yesSouth-East European Forestry, 2020
The saprotrophic, endophytic, and parasitic fungi were detected from the samples collected in the forest of the management unit East Psunj and Papuk Nature Park in Croatia.
Nevenka Ćelepirović   +2 more
doaj  

Fungal Antimicrobial Resistance: Mechanisms, Drivers, and Global Clinical Burden

open access: yesChemFoodChem, EarlyView.
ABSTRACT Fungal antimicrobial resistance (AMR) is a growing concern for world health caused by an increase in multidrug‐resistant infections, an increase in environmental reservoirs, and the ineffectiveness of current antifungal treatments. Fungal infections continue to be largely excluded from AMR initiatives while causing over 1.6 million deaths ...
Bikash Baral
wiley   +1 more source

Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification

open access: yesOpen Life Sciences
Genomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the ...
Dar Gulam Jeelani   +5 more
doaj   +1 more source

Fungal DNA Lysis Buffer v1

open access: yes, 2016
Lysis buffer for extraction of DNA from fungal material. Contains ionic and non-ionic detergents. Contains RNAse-A. Composition: NaCl 150mM EDTA pH 8.0 1 mM Tris BasepH 8.0 10 mM Polyvinylpolypyrrolidone (PVPP) 2% IGEPAL-CA630 0.5% Sodium deoxycholate 0.5% SDS 1% RNAse-A 100 µg/mL
openaire   +1 more source

Optimization of CEST MRI Reporter Protein Design Using Cation‐Pi Networks

open access: yesChemistry – A European Journal, EarlyView.
A novel engineering approach can produce reporter proteins for cell and viral therapy tracking with unique magnetic resonance imaging (MRI) signatures, detectable with chemical exchange saturation transfer (CEST). We discover how cation‐π interactions between amino acid groups can help us fine‐tune magnetic resonance properties for noninvasive ...
David E. Korenchan   +8 more
wiley   +1 more source

Monosubstituted N‐Arylhydroxylamine Chemistry: Integrating Contemporary Synthetic Approaches for the Efficient Construction of Diverse Heterocyclic Scaffolds

open access: yesChemistry – A European Journal, EarlyView.
Monosubstituted N‐arylhydroxylamines represent a unique subclass of hydroxylamines that act as pivotal intermediates in redox transformations and as versatile platforms for further synthetic transformations. They serve as key building blocks in the synthesis of architecturally complex heterocycles and other valuable organic compounds.
Michael G. Kallitsakis   +2 more
wiley   +1 more source

PEK14: A Kinesin‐4 Necessary for Male‐Derived Fertility in Arabidopsis thaliana

open access: yesCytoskeleton, EarlyView.
ABSTRACT Of the 61 kinesins annotated in Arabidopsis thaliana, many are still without assigned function. Here, we have screened an insertional mutant library of Arabidopsis pollen‐expressed kinesins for fertility defects. Insertional mutants for three kinesins showed a significant reduction in seed set.
Isabella N. Mendes   +6 more
wiley   +1 more source

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