Results 141 to 150 of about 1,916,213 (308)

Comparative Evaluation of DNA Extraction Protocols for Filamentous Fungi Using Cost-Effective and Broadly Accessible Methods

African and Mediterranean Agricultural Journal - Al Awamia
Reliable extraction of high-quality genomic DNA is essential for accurate molecular identification, population genetics, and plant disease diagnostics in filamentous fungi. However, DNA recovery is often hampered by the complex and resistant structure of
Imane Hourmatallah, Faical Aoujil, Hajar Hamzaoui, Chaimae El Ghdaich, Sanae Guissi, Khaoula Habbadi   +5 more
doaj   +1 more source

Fungal DNA Lysis Buffer v1

, 2016
Lysis buffer for extraction of DNA from fungal material. Contains ionic and non-ionic detergents. Contains RNAse-A. Composition: NaCl 150mM EDTA pH 8.0 1 mM Tris BasepH 8.0 10 mM Polyvinylpolypyrrolidone (PVPP) 2% IGEPAL-CA630 0.5% Sodium deoxycholate 0.5% SDS 1% RNAse-A 100 µg/mL
openaire   +1 more source

Induction and Enhancement of Bacteriocin Production (Review)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification

Open Life Sciences
Genomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the ...
Dar Gulam Jeelani, Nazir Ruqeya, Wani Shakil A., Farooq Saleem, Aziz Tariq, Albekairi Thamer H.   +5 more
doaj   +1 more source

DNA Barcoding of Fungi in the Forest Ecosystem of the Psunj and Papuk Mountains in Croatia

South-East European Forestry, 2020
The saprotrophic, endophytic, and parasitic fungi were detected from the samples collected in the forest of the management unit East Psunj and Papuk Nature Park in Croatia.
Nevenka Ćelepirović, Sanja Novak Agbaba, Monika Karija Vlahović   +2 more
doaj  

Targeting neutrophil extracellular traps in metabolic and immune niche: Nanomaterials for diabetes tissue regeneration

BMEMat, EarlyView.
The effects of NETs on regeneration of various diabetic tissues, and strategies targeting NETs for diabetes tissue regeneration. In the diabetic environment, NETs undergo complex metabolic and immune reprogramming, leading to dynamic changes in antibacterial and proinflammatory functions, and affecting regeneration of multiple systemic tissues.
Xinyi Jiang, Muxin Yue, Kai Mao, Boon Chin Heng, Noor Azlin Yahya, Yunsong Liu, Zheng Li   +6 more
wiley   +1 more source

Advanced nanotherapeutics for combating ocular infections: Rational design, antimicrobial mechanisms, and therapeutic breakthroughs

BMEMat, EarlyView.
Nanomaterials revolutionize ocular infection treatment by enabling targeted drug delivery and enhanced antimicrobial efficacy against resistant pathogens. This review systematically explores their rational design, multimodal mechanisms, and translational potential for next‐generation anti‐infective therapies.
Yujia Liu, Xuetian Wen, Yue Tan, Rui Zeng, Yijuan Zhang, Tong Lin, Lan Gong, Zichao Luo   +7 more
wiley   +1 more source

DGGE coupled with ribosomal DNA gene phylogenies reveal uncharacterized fungal phylotypes

, 2006
Most fungal diversity studies have previously been based on morphological examination and cultivation methods. In this study we use a molecular method based on DGGE coupled with sequence analysis of 18S rRNA gene to assess fungal diversity on leaves of ...
Hyde, KD, Jeewon, R, Lumyong, S, Duong, LM   +3 more
core  

Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees.

PLoS ONE
Effective culturing and DNA extraction protocols are essential for advancing research on fungal pathogens of brown spot needle blight (BSNB) that infect loblolly pine (Pinus taeda) and other Pinus species. We evaluated the performance of four widely used
Temitope Ruth Folorunso, Gabriel Silva, Marilis E Girón, Tess Lindow, Micah Persyn, Lori Eckhardt, Janna R Willoughby   +6 more
doaj   +1 more source

Quick Fungal DNA Extraction v1

, 2016

openaire   +1 more source