Results 1 to 10 of about 947,997 (188)

Plant DNA polymerases [PDF]

International Journal of Molecular Sciences, 2019
Maintenance of genome integrity is a key process in all organisms. DNA polymerases (Pols) are central players in this process as they are in charge of the faithful reproduction of the genetic information, as well as of DNA repair.
De Veylder, Lieven, Pedroza-Garcia, José Antonio, Raynaud, Cécile   +2 more
core   +9 more sources

A fully automatable enzymatic method for DNA extraction from plant tissues [PDF]

BMC Plant Biology, 2005
Background DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall around the plant cells.
Markov Alexander V, Aeschbach Lorène, Sinitsyna Olga, Manen Jean-François, Sinitsyn Arkady   +4 more
doaj   +3 more sources

XdfA, a novel membrane-associated DedA family protein of Xanthomonas campestris, is required for optimum virulence, maintenance of magnesium, and membrane homeostasis

mBio, 2023
Xanthomonas campestris is an important member of the Xanthomonas group of phytopathogens that causes diseases in crucifers. In X. campestris, several virulence-associated functions, including some belonging to unknown predicted functions, have been ...
Yasobanta Padhi, Subhadeep Chatterjee
doaj   +1 more source

Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants

Genome Biology, 2023
Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9.
Liyang Zhang, Gen Li, Yingxiao Zhang, Yanhao Cheng, Nathaniel Roberts, Steve E. Glenn, Diane DeZwaan-McCabe, H. Tomas Rube, Jeff Manthey, Gary Coleman, Christopher A. Vakulskas, Yiping Qi   +11 more
doaj   +1 more source

Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

Frontiers in Genome Editing, 2022
Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target editing effects, mitigation of random integration of non-native ...
Raviraj Banakar, Raviraj Banakar, Raviraj Banakar, Mollie Schubert, Gavin Kurgan, Krishan Mohan Rai, Krishan Mohan Rai, Krishan Mohan Rai, Sarah F. Beaudoin, Michael A. Collingwood, Christopher A. Vakulskas, Kan Wang, Kan Wang, Feng Zhang, Feng Zhang, Feng Zhang   +15 more
doaj   +1 more source

Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]

, 1987
Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
A Crossway, A Deshayes, B Reiss, BR Thomas, C-L Hsiao, CP Meredith, DT Stinchcomb, EA Shahin, FA Krens, G Scangos, HC Birnboim, HE Ruley, I Potrykus, J Messing, J Paszkowski, Jacques Hille, JD Rochaix, K Struhl, K Wernars, KC Reed, KR Folger, M Koornneef, M Perucho, Maarten Jongsma, Maarten Koornneef, MG Murray, MW Bevan, O Smithies, P Zabel, Pim Zabel, PW Rigby, R Hain, R Peerbolte, RA Anderson, RD Shillito, S Kato, SN Cohen, T Maniatis, TL Adams   +38 more
core   +15 more sources

Cherry Blossom Forecast Based on Transcriptome of Floral Organs Approaching Blooming in the Flowering Cherry (Cerasus × yedoensis) Cultivar ‘Somei-Yoshino’

Frontiers in Plant Science, 2022
To gain insights into the genetic mechanisms underlying blooming and petal movement in flowering cherry (Cerasus × yedoensis), we performed time-course RNA-seq analysis of the floral buds and open-flowers of the most popular flowering cherry cultivar ...
Kenta Shirasawa, Tomoya Esumi, Akihiro Itai, Sachiko Isobe   +3 more
doaj   +1 more source

Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases

Frontiers in Genome Editing, 2022
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and ...
Yingxiao Zhang, Yanhao Cheng, Yanhao Cheng, Hong Fang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, Randall P. Niedz, James N. Culver, James N. Culver, Yiping Qi, Yiping Qi   +11 more
doaj   +1 more source

AIP1 is a novel Agenet/Tudor domain protein from Arabidopsis that interacts with regulators of DNA replication, transcription and chromatin remodeling [PDF]

, 2015
Background: DNA replication and transcription are dynamic processes regulating plant development that are dependent on the chromatin accessibility. Proteins belonging to the Agenet/Tudor domain family are known as histone modification "readers" and ...
Barroso-Neto, Ito Liberato, Brasil, Juliana Nogueira, Cabral, Luiz Mors, Eloy, Nubia Barbosa, Ferreira, Paulo CG, Gonzalez Sanchez, Nathalie, Hemerly, Adriana S, Inzé, Dirk, Perdigão Grangeiro, Leticia P, Primo, Luiza MF   +9 more
core   +3 more sources

DNA Methylation in Acacia mangium ln vitro and Ex-vitro buds, in relation to their within-shoot position, age and leaf morphology of the shoots [PDF]

, 2009
DNA methylation was analyzed in Acacia mangium Willd. buds collected from in vitro and field growing shoots from the same clone and its juvenile offspring.
Baurens, Franc-Christophe, Doulbeau, Sylvie, Goh, Doreen Kim Soh, Monteuuis, Olivier, Quimado, M., Verdeil, Jean-Luc   +5 more
core   +1 more source