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Plant Organelle DNA Maintenance [PDF]

Plants, 2020
Plant cells contain two double membrane bound organelles, plastids and mitochondria, that contain their own genomes. There is a very large variation in the sizes of mitochondrial genomes in higher plants, while the plastid genome remains relatively ...
Niaz Ahmad, Brent L. Nielsen
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XdfA, a novel membrane-associated DedA family protein of Xanthomonas campestris, is required for optimum virulence, maintenance of magnesium, and membrane homeostasis

mBio, 2023
Xanthomonas campestris is an important member of the Xanthomonas group of phytopathogens that causes diseases in crucifers. In X. campestris, several virulence-associated functions, including some belonging to unknown predicted functions, have been ...
Yasobanta Padhi, Subhadeep Chatterjee
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Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants

Genome Biology, 2023
Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9.
Liyang Zhang, Gen Li, Yingxiao Zhang, Yanhao Cheng, Nathaniel Roberts, Steve E. Glenn, Diane DeZwaan-McCabe, H. Tomas Rube, Jeff Manthey, Gary Coleman, Christopher A. Vakulskas, Yiping Qi   +11 more
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Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

Frontiers in Genome Editing, 2022
Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target editing effects, mitigation of random integration of non-native ...
Raviraj Banakar, Raviraj Banakar, Raviraj Banakar, Mollie Schubert, Gavin Kurgan, Krishan Mohan Rai, Krishan Mohan Rai, Krishan Mohan Rai, Sarah F. Beaudoin, Michael A. Collingwood, Christopher A. Vakulskas, Kan Wang, Kan Wang, Feng Zhang, Feng Zhang, Feng Zhang   +15 more
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Cherry Blossom Forecast Based on Transcriptome of Floral Organs Approaching Blooming in the Flowering Cherry (Cerasus × yedoensis) Cultivar ‘Somei-Yoshino’

Frontiers in Plant Science, 2022
To gain insights into the genetic mechanisms underlying blooming and petal movement in flowering cherry (Cerasus × yedoensis), we performed time-course RNA-seq analysis of the floral buds and open-flowers of the most popular flowering cherry cultivar ...
Kenta Shirasawa, Tomoya Esumi, Akihiro Itai, Sachiko Isobe   +3 more
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Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases

Frontiers in Genome Editing, 2022
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and ...
Yingxiao Zhang, Yanhao Cheng, Yanhao Cheng, Hong Fang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, Randall P. Niedz, James N. Culver, James N. Culver, Yiping Qi, Yiping Qi   +11 more
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Genetic engineering to improve quality, productivity and value of crops

California Agriculture, 2000
Over the next 25 years, we believe that the most significant changes in crops will come about by applying genetic engineering tools. Crops may be bioengineered to produce modified kinds of starch, oils and high-value proteins for better
Abhaya Dandekar, Neal Gutterson
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Development of BC₃F₂ Tomato Genotypes with Arthropod Resistance Introgressed from Solanum habrochaites var. hirsutum (PI127826)

Horticulturae, 2022
Arthropod pests are among the biggest problems faced in tomato production worldwide. To overcome the losses caused by these pests, one of the most sustainable and economical strategies is the use of resistance introgressed from wild species.
Flávia Cristina Panizzon Diniz, Juliano Tadeu Vilela de Resende, Renato Barros de Lima-Filho, Laura Pilati, Gabriella Correia Gomes, Sergio Ruffo Roberto, Paulo Roberto Da-Silva   +6 more
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Chromosome-level genome assembly of Ophiorrhiza pumila reveals the evolution of camptothecin biosynthesis

Nature Communications, 2021
Ophiorrhiza pumila is a medicinal plant that can produce the anti-cancer monoterpene indole alkaloid (MIA) camptothecin. Here, the authors report its genome assembly and propose a working model for MIA evolution and biosynthesis through comparative ...
Amit Rai, Hideki Hirakawa, Ryo Nakabayashi, Shinji Kikuchi, Koki Hayashi, Megha Rai, Hiroshi Tsugawa, Taiki Nakaya, Tetsuya Mori, Hideki Nagasaki, Runa Fukushi, Yoko Kusuya, Hiroki Takahashi, Hiroshi Uchiyama, Atsushi Toyoda, Shoko Hikosaka, Eiji Goto, Kazuki Saito, Mami Yamazaki   +18 more
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Haplotyping the Vitis collinear core genome with rhAmpSeq improves marker transferability in a diverse genus

Nature Communications, 2020
Trait introgression requires universal markers, but cross-species transferability of current SNP markers can be as low as 2%. Here, the authors use an AmpSeq haplotype strategy targeting the collinear core genome for marker development and show ...
Cheng Zou, Avinash Karn, Bruce Reisch, Allen Nguyen, Yongming Sun, Yun Bao, Michael S. Campbell, Deanna Church, Stephen Williams, Xia Xu, Craig A. Ledbetter, Sagar Patel, Anne Fennell, Jeffrey C. Glaubitz, Matthew Clark, Doreen Ware, Jason P. Londo, Qi Sun, Lance Cadle-Davidson   +18 more
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