Results 71 to 80 of about 412,644 (246)
Molecular Plant-Microbe Interactions, 2000 Using the Cre/lox recombination system, we analyzed the extent to which T-DNA transfer to the plant cell and T-DNA integration into the plant genome determine the transformation and cotransformation frequencies of Arabidopsis root cells.
Sylvie De Buck +3 more
doaj +1 more source
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
Food Technology and Biotechnology, 2013 In vitro rhizogenesis occurred with a characteristic pattern typical of transformed roots following explant (internode/leaf) inoculation of Amaranthus spinosus L. with four different wild type Agrobacterium rhizogenes strains.
Ajantaa Pal +3 more
doaj
A fully automatable enzymatic method for DNA extraction from plant tissues
BMC Plant Biology, 2005 Background DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall around the plant cells.
Markov Alexander V +4 more
doaj +1 more source
Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions
FEBS Letters, EarlyView.We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva +10 more
wiley +1 more source
So sánh và đánh giá các phương pháp ly trích DNA trong các sản phẩm có nguồn gốc từ hạt ca cao
Tạp chí Khoa học Đại học Cần Thơ, 2019 Bốn phương pháp trích ly DNA nhanh (kit) được dùng trong nghiên cứu đánh giá và so sánh nhằm chọn phương pháp trích ly DNA tối ưu nhất cho từng nguyên liệu có nguồn gốc từ sản phẩm ca cao (bốn nguyên liệu sử dụng trong thí nghiệm: bột ca cao, bơ ca cao ...
Lâm Thị Việt Hà +3 more
doaj +1 more source
FEBS Letters, EarlyView.The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li +2 more
wiley +1 more source
The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms
Communications BiologyIn eukaryotes, chromosome segregation relies on attachment to the spindle, ensured by the kinetochore. The outer kinetochore attaches to the microtubules and is named after three sub-complexes KNL1C, MIS12C, and NDC80C (KMN).
Dipesh Kumar Singh +7 more
doaj +1 more source
PICALM::MLLT10 translocated leukemia
FEBS Letters, EarlyView.This comprehensive review of PICALM::MLLT10 translocated acute leukemia provides an in‐depth review of the structure and function of CALM, AF10, and the fusion oncoprotein (1). The multifaceted molecular mechanisms of oncogenesis, including nucleocytoplasmic shuttling (2), epigenetic modifications (3), and disruption of endocytosis (4), are then ...
John M. Cullen +7 more
wiley +1 more source
Plant MethodsBackground There is a growing demand for fast and reliable plant biomolecular analyses. DNA extraction is the major bottleneck in plant nucleic acid-based applications especially due to the complexity of tissues in different plant species.
Shashini De Silva +4 more
doaj +1 more source