Results 251 to 260 of about 611,894 (312)

Atox1 promotes CRC progression by protecting DNA damage through interacts with a novel copper-binding protein PARP1. [PDF]

open access: yesCell Commun Signal
Sun M   +12 more
europepmc   +1 more source

Selected Pages from Mailing I & II sent to participants prior to the Recombinant DNA Advisory Committee meeting, December 8-9- 10, 1999.

open access: yes, 1999
National Institutes of Health [NIH]. (United States). Recombinant DNA Advisory Committee
core  

Including "Points to Consider in the Design and Submission of Protocols for the Transfer of Recombinant DNA into Human Subjects" Minutes of 21 June 1989 Meeting

open access: yes, 1989
National Institutes of Health [NIH] (United States). Recombinant DNA Advisory Committee. Human Gene Therapy Subcommittee
core  

Recombinant DNA technical bulletin

open access: yes
National Institute of General Medical Sciences (U.S.). Office of Recombinant DNA Activities.   +2 more
core  

Scarless DNA Recombineering

Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi   +2 more
openaire   +2 more sources

Recombination in yeast and the recombinant DNA technology

Genome, 1989
The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
T D, Petes   +9 more
openaire   +2 more sources

Introduction to recombinant DNA

Journal of Inherited Metabolic Disease, 1986
AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
openaire   +2 more sources

DNA Recombineering Applications

Cold Spring Harbor Protocols, 2023
The ability to manipulate the bacterial genome is an obligatory premise for the study of gene function and regulation in bacterial cells. The λ red recombineering technique allows modification of chromosomal sequences with base-pair precision without the need of intermediate molecular cloning steps.
Nara Figueroa-Bossi   +2 more
openaire   +2 more sources

The recombinant DNA technology

Journal of Veterinary Pharmacology and Therapeutics, 1983
The recombinant DNA technology or DNA cloning permits the isolation, amplification, and precise manipulation of specific DNA fragments. This is generally accomplished by linking or recombining the desired DNA fragment with a DNA molecule, termed the vector, which is capable of directing the replication of itself in a suitable host cell and any DNA ...
openaire   +2 more sources

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