Results 251 to 260 of about 614,049 (312)

A versatile plasmid platform for auxotrophic complementation in attenuated Mycobacterium bovis BCG. [PDF]

Mol Biol Rep
Madruga AB, Nogueira JD, Maia MAC, de Oliveira NR, Ehlert ME, Ferreira VG, Pacheco BS, Seixas FK, Collares TV, Acosta IB, Varela AS, Dellagostin OA, Bohn TLO.   +12 more
europepmc   +1 more source

Chemiluminescent Deoxyribozyme Sensors for DNA-Editing Enzymes. [PDF]

ACS Chem Biol
Jakubec M, Svoboda M, Kurfürst J, Svehlova K, Veverka V, Curtis EA.   +5 more
europepmc   +1 more source

Enhanced virulence of Autographa californica multiple nucleopolyhedrovirus in <i>Spodoptera frugiperda</i> is mediated by an Ac34 mutation that promotes nucleocapsid envelopment within occlusion bodies. [PDF]

J Virol
Jin W, Guo L, Tong Y, Shi H, Ganbold U, Kang Q, Meng Q, Zhang J, Qin Q, Zhang H.   +9 more
europepmc   +1 more source

Recombinant DNA technical bulletin [PDF]

National Institute of General Medical Sciences (U.S.). Office of Recombinant DNA Activities., National Institutes of Health (U.S.). Office of Recombinant DNA Activities., National Institute of Allergy and Infectious Diseases (U.S.). Office of Recombinant DNA Activities.   +2 more
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Mechanistic insight into complement C3 regulation during chronic HBV infection: effect on viral persistence and host immune response. [PDF]

J Biomed Sci
Baidya A, Dey D, Mallik S, Halder S, Khatun N, Jha R, Nandi S, Chakraborty BC, Dutta A, Banerjee S, Chowdhury A, Ahammed SKM, Datta S.   +12 more
europepmc   +1 more source

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Scarless DNA Recombineering

Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi, Roberto Balbontín, Lionello Bossi   +2 more
openaire   +2 more sources

Recombination in yeast and the recombinant DNA technology

Genome, 1989
The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
T D, Petes, P, Detloff, S, Jinks-Robertson, S R, Judd, M, Kupiec, D, Nag, A, Stapleton, L S, Symington, A, Vincent, M, White   +9 more
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Introduction to recombinant DNA

Journal of Inherited Metabolic Disease, 1986
AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
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DNA Recombineering Applications

Cold Spring Harbor Protocols, 2023
The ability to manipulate the bacterial genome is an obligatory premise for the study of gene function and regulation in bacterial cells. The λ red recombineering technique allows modification of chromosomal sequences with base-pair precision without the need of intermediate molecular cloning steps.
Nara Figueroa-Bossi, Roberto Balbontín, Lionello Bossi   +2 more
openaire   +2 more sources

The recombinant DNA technology

Journal of Veterinary Pharmacology and Therapeutics, 1983
The recombinant DNA technology or DNA cloning permits the isolation, amplification, and precise manipulation of specific DNA fragments. This is generally accomplished by linking or recombining the desired DNA fragment with a DNA molecule, termed the vector, which is capable of directing the replication of itself in a suitable host cell and any DNA ...
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