High-throughput FastCloning technology: A low-cost method for parallel cloning
PLoS ONE, 2022 FastCloning, a reliable cloning technique for plasmid construction, is a widely used protocol in biomedical research laboratories. Only two-step molecular manipulations are required to add a gene (cDNA) of interest into the desired vector.
Hua Jiang +9 more
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Stem Cell Research & Therapy, 2019 Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu +13 more
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Molecular cloning of cat interleukin-12 [PDF]
Immunogenetics, 1997 The recently discovered interleukin-12 (IL-12) is a heterodimeric 70000 Mr cytokine composed of a disulphide-bonded 35000 Mr (p35) subunit and a 40000 Mr (p40) subunit. Produced mainly by activated monocytes/macrophages and B cells, it enhances NK/LAK cell cytolytic activity and stimulates cytotoxic T-cell responses and IFN-γ production.
Horzinek, M.C. +3 more
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Arguments for Human Therapeutic Cloning
Biomolecules & Biomedicine, 2008 For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
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Molecular cloning and characterization of PLC‐η2 [PDF]
The FASEB Journal, 2005 Phospholipase C isozymes catalyze the conversion of phosphatidylinositol‐(4,5)P 2 to the Ca 2+ ‐mobilizing second messenger, Ins(1,4,5)P 3 , and the protein kinase C‐activating second messenger, diacylglycerol ...
Yixing, Zhou +3 more
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Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning
, 2010 Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker +15 more
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Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research. [PDF]
PLoS ONE, 2017 Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding ...
Mostafa Aboulela +7 more
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cDNA Cloning Demonstrates the Expression of Pregnancy-Specific Glycoprotein Genes, a Subgroup of the Carcinoembryonic Antigen Gene Family, in Fetal Liver [PDF]
, 1989 The pregnancy-specific glycoprotein (PSG) genes constitute a subgroup of the carcinoembryonic antigen (CEA) gene family. Here we report the cloning of four cDNAs coding for different members of the PSG family from a human fetal liver cDNA library.
Zimmermann, Wolfgang +2 more
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Recombination-assisted megaprimer (RAM) cloning
MethodsX, 2014 No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide ...
Jacques Mathieu +2 more
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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR. [PDF]
PLoS ONE, 2017 The precise assembly of defined DNA sequences into plasmids is an essential task in bioscience research. While a number of molecular cloning techniques have been developed, many methods require specialized expensive reagents or laborious experimental ...
Faqing Huang +2 more
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