Results 11 to 20 of about 3,746,077 (303)

Porcine SLITRK1: Molecular cloning and characterization [PDF]

FEBS Open Bio, 2014
The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The
Knud Larsen, Jamal Momeni, Leila Farajzadeh, Christian Bendixen   +3 more
doaj   +4 more sources

Versatile vector tools for efficient protein screening across multiple expression systems [PDF]

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +2 more sources

Towards an understanding of British public attitudes concerning human cloning. [PDF]

, 2007
The ability of scientists to apply cloning technology to humans has provoked public discussion and media coverage. The present paper reports on a series of studies examining public attitudes to human cloning in the UK, bringing together a range of ...
Coyle, Adrian, Senior, V, Walton, Chris, Senior, Victoria, Shepherd, Richard, Walton, C, Barnett, J, Moran-Ellis, J, Barnett, Julie, Shepherd, R, Coyle, A, Cooper, H, Cooper, Helen, Moran-Ellis, Jo   +13 more
core   +5 more sources

Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses

Microbiology Spectrum, 2023
Enteroviruses (EVs) have been associated with several human diseases. Due to their continuous emergence and divergence, EV species have generated more than 100 types and recombinant strains, increasing the public health threat caused by them.
Won-Suk Choi, Sol Oh, Khristine Joy C. Antigua, Ju Hwan Jeong, Beom Kyu Kim, Yu Soo Yun, Da Hyeon Kang, Seong Cheol Min, Byung-Kwan Lim, Won Seop Kim, Ji-Hyuk Lee, Eung-Gook Kim, Young Ki Choi, Yun Hee Baek, Min-Suk Song   +14 more
doaj   +1 more source

Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells

STAR Protocols, 2022
Summary: Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures.
Ana Luiza Drumond-Bock, Magdalena Cybula, Luyao Wang, Lin Wang, Magdalena Bieniasz   +4 more
doaj   +1 more source

A practical comparison of ligation-independent cloning techniques. [PDF]

PLoS ONE, 2013
The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and ...
Julian Stevenson, James R Krycer, Lisa Phan, Andrew J Brown   +3 more
doaj   +1 more source

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

Frontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan, Yanjing Zeng, Wentao Shen, Decai Tuo, Xiaoying Li, Peng Zhou   +5 more
doaj   +1 more source

Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method [PDF]

PeerJ, 2018
Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min.
Lendl Tan, Emily J. Strong, Kyra Woods, Nicholas P. West   +3 more
doaj   +2 more sources

Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students

Journal of Microbiology & Biology Education, 2023
Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes.
Mihaela Bejenari, Line Nielsen, Eva Mie Lang Spedtsberg, Mikkel Rank Nielsen, Tobias Bruun Pedersen, Jens Laurids Sørensen   +5 more
doaj   +1 more source

Streamlined assembly of cloning and genome editing vectors for genus Clostridium

iScience, 2023
Summary: Reported herein is a new set of vectors designed to streamline molecular cloning and genome editing by exploiting modern cloning methods. The new vectors build on the existing pMTL8000 vectors that have been a staple of Clostridium research for ...
Tom S. Bailey, Philip Hittmeyer, Yanchao Zhang, Aleksandra M. Kubiak   +3 more
doaj   +1 more source