Results 11 to 20 of about 805,120 (315)

El análisis molecular y el inmunogénico sugieren la ausencia de las proteínas hidrofílicas de superficie en Leishmania (Viannia) panamensis

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2008
Introducción. Los dos subgéneros en los cuales se divide el género Leishmania: Viannia y Leishmania, presentan diferencias significativas en las manifestaciones clínicas que causan, en su comportamiento de crecimiento en cultivos in vitro, en sus ...
Marcel Marín   +4 more
doaj   +1 more source

Diversity of actinobacteria in the marshes of Ezzemoul and Djendli in northeastern Algeria [PDF]

open access: yes, 2019
The main purpose of this research is to study the microbial diversity of actinobacteria, living in “Ezzemoul” and “Djendli” sebkhas soils. These salt lakes are situated in the east of Algeria and they are microbiologically underexploited.
Kitouni, Mahmoud, Smati, Maria
core   +2 more sources

PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs

open access: yesBioTechniques, 2023
With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran   +4 more
doaj   +1 more source

The Cloning of the Human Tumor Supressor Gene INGI: DNA Cloning into Plasmid Vector and DNA Analysis by Restriction Enzymes

open access: yesJournal of Dentistry Indonesia, 2015
DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari   +2 more
doaj   +1 more source

Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site [PDF]

open access: yes, 2018
Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Beyaert, Rudi   +2 more
core   +1 more source

QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids [PDF]

open access: yes, 2015
Background Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning.
Jajesniak, P., Wong, T.S.
core   +2 more sources

Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting

open access: yesiScience, 2019
Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé   +2 more
doaj   +1 more source

HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review

open access: yesBiomedicines, 2021
Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates ...
Kiichi Hirota
doaj   +1 more source

Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site

open access: yesBioTechniques, 2019
Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal   +4 more
doaj   +1 more source

Local cloning of two product states [PDF]

open access: yes, 2005
Local quantum operations and classical communication (LOCC) put considerable constraints on many quantum information processing tasks such as cloning and discrimination.
Mingsheng Ying   +3 more
core   +2 more sources

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