Results 11 to 20 of about 485,373 (308)
, 2022 This book examines the fundamentals of molecular cloning and molecular cloning applications in various areas. Chapters address such topics as tools and methodologies of molecular cloning, molecular cloning for medicine, food and feed, the environment ...
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Journal of Dentistry Indonesia, 2015 DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari +2 more
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Porcine SLITRK1: Molecular cloning and characterization [PDF]
FEBS Open Bio, 2014 The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The
Knud Larsen +3 more
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Molecules and Cells: Analysis and manipulation of DNA is fundamental to understand gene function and expression. Gene cloning is a routine and versatile technique for molecular biology, allowing isolation, amplification, and production of recombinant DNA molecules.
Woojin Hong +3 more
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Human Genetics, 1977 Biochemical, biophysical and genetic studies of DNA segments of complex genomes are greatly facilitated by a variety of techniques, called molecular cloning of DNA, which permit propagation of single DNA segments of virtually any origin in bacterial ...
Vosberg, H., Hans-Peter Vosberg
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Construction and application of an insertional activation cloning vector [PDF]
BMC BiotechnologyBackground Foreign DNA cloning is a critical step in genetic engineering and molecular biology for acquiring target genes. Efficient and accurate identification of positive clones from transformants is essential to this process.
Yan Zhang +4 more
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Current Issues in Molecular BiologyTo clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. Some cloning vectors have been developed for the cloning of PCR products, including blunt-end and T-A cloning. However, different plasmids are required
Xingli Zhang +4 more
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Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses
Microbiology Spectrum, 2023 Enteroviruses (EVs) have been associated with several human diseases. Due to their continuous emergence and divergence, EV species have generated more than 100 types and recombinant strains, increasing the public health threat caused by them.
Won-Suk Choi +14 more
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STAR Protocols, 2022 Summary: Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures.
Ana Luiza Drumond-Bock +4 more
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A practical comparison of ligation-independent cloning techniques. [PDF]
PLoS ONE, 2013 The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and ...
Julian Stevenson +3 more
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