Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning
Frontiers in Bioengineering and Biotechnology, 2020 Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan +5 more
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Molecular cloning and functional characterization of the shikimate kinase gene from Baphicacanthus cusia [PDF]
Frontiers in Plant ScienceBaphicacanthus cusia (Nee) Bremek, a perennial herbaceous plant with medicinal properties, has limited genomic insights regarding the genes involved in its indole alkaloid biosynthesis pathway.
Yuxiang Huang +10 more
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Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses
Microbiology Spectrum, 2023 Enteroviruses (EVs) have been associated with several human diseases. Due to their continuous emergence and divergence, EV species have generated more than 100 types and recombinant strains, increasing the public health threat caused by them.
Won-Suk Choi +14 more
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STAR Protocols, 2022 Summary: Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures.
Ana Luiza Drumond-Bock +4 more
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A practical comparison of ligation-independent cloning techniques. [PDF]
PLoS ONE, 2013 The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and ...
Julian Stevenson +3 more
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Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method [PDF]
PeerJ, 2018 Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min.
Lendl Tan +3 more
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Journal of Microbiology & Biology Education, 2023 Recombinant plasmids are essential tools in molecular biotechnology, and reliable plasmid assembly methods have, therefore, become a prerequisite for the successful cloning and transfer of genes.
Mihaela Bejenari +5 more
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Streamlined assembly of cloning and genome editing vectors for genus Clostridium
iScience, 2023 Summary: Reported herein is a new set of vectors designed to streamline molecular cloning and genome editing by exploiting modern cloning methods. The new vectors build on the existing pMTL8000 vectors that have been a staple of Clostridium research for ...
Tom S. Bailey +3 more
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HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs. [PDF]
PLoS ONE, 2014 Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in ...
Ming V Li +6 more
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Isolation, Molecular Cloning, and Expression of yyxA Serine Protease Gene Extracted from Bacillus licheniformis in Escherichia coli [PDF]
مجله بیوتکنولوژی کشاورزی, 2021 Objective Proteases are among the most important industrial enzymes. Microbial proteases, especially from Bacillus sp., are most widely exploited industrially. The aim of this study was isolation, cloning, sequencing, expression, and bioinformatics study
Zahra Aghaei Jeshvaghani, Ramin Hoseini
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