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This book examines the fundamentals of molecular cloning and molecular cloning applications in various areas. Chapters address such topics as tools and methodologies of molecular cloning, molecular cloning for medicine, food and feed, the environment ...
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: Analysis and manipulation of DNA is fundamental to understand gene function and expression. Gene cloning is a routine and versatile technique for molecular biology, allowing isolation, amplification, and production of recombinant DNA molecules.
Woojin Hong +3 more
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Porcine SLITRK1: Molecular cloning and characterization [PDF]
The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The
Knud Larsen +3 more
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The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day.
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With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran +4 more
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Primers for molecular cloning.
Primers for molecular cloning.
Herve Migaud (308426) +5 more
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Molecular cloning of a bovine cathepsin [PDF]
A cDNA clone for a thiol endoproteinase has been isolated from a bovine heart cDNA library by using a mixture of 32 synthetic oligonucleotides as a hybridization probe. The inserted region is 672 base pairs in length. It contains a sequence encoding the C-terminal region of a protein that is homologous to rat liver cathepsins B and H and to plant thiol
N J, Gay, J E, Walker
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DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari +2 more
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Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé +2 more
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HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review
Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates ...
Kiichi Hirota
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